于龙川
(1)神经肽及其受体在中枢痛觉信息传递和调节中的作用和机制;(2)阿片类耐受和依赖的神经机制;(3)学习记忆的神经生物学机制,特别是神经肽及其受体在老年性痴呆(Alzheimer’s Disease)防治中的作用。
个性化签名
- 姓名:于龙川
- 目前身份:
- 担任导师情况:
- 学位:
-
学术头衔:
博士生导师
- 职称:-
-
学科领域:
光学
- 研究兴趣:(1)神经肽及其受体在中枢痛觉信息传递和调节中的作用和机制;(2)阿片类耐受和依赖的神经机制;(3)学习记忆的神经生物学机制,特别是神经肽及其受体在老年性痴呆(Alzheimer’s Disease)防治中的作用。
于龙川,北京大学生命科学学院,教授,博士生导师,神经生物学实验室负责人。1983年在山东医学院获得医学硕士学位;1987年在北京医科大学获得博士学位;1998年在瑞典Karolinska 研究院获得医学科学博士(Medical Science Doctor);1987-1989年在北京大学做博士后研究。1993-1995,和1997-1998年在瑞典Karolinska 研究院生理学与药理学系作访问学者;1999-2000年在美国加州大学旧金山分校(UCSF)作访问学者。 回国后在1996年组建神经生物学实验室,建立了脑内定点给药,细胞外多管微电极记录和给药,膜片钳全细胞记录,免疫组织化学和细胞荧光组织化学,细胞培养,痛觉行为学测量,Morris 水迷宫,位置偏爱反应等有关的实验方法。实验室主要研究(1)神经肽及其受体在中枢痛觉信息传递和调节中的作用和机制;(2)阿片类耐受和依赖的神经机制;(3)学习记忆的神经生物学机制,特别是神经肽及其受体在老年性痴呆(Alzheimer’s Disease)防治中的作用。自1996年以来于龙川教授获得多项国家自然科学基金和教育部博士点基金的资助,自1996年以来在国内发表论文6篇,在国际学术杂志 (Pain, Neuroscience, Br. J. Pharmacol., J Neuroscience Research等)上发表科研论文五十余篇, 引起国际同行的关注。
-
主页访问
6394
-
关注数
0
-
成果阅读
939
-
成果数
19
【期刊论文】Roles of oxytocin in spatial learning and memory in the nucleus basalis of Meynert in rats
于龙川, Wei Wu, Long-Chuan Yu*
Regulatory Peptides 120(2004)119-125,-0001,():
-1年11月30日
The present study was performed to explore the role of oxytocin (OT) in spatial learning and memory in the nucleus basalis of Meynert (NBM) of rats. The latency, distance and swimming path to find the platform were tested by Morris water maze and recorded by a video camera connected to a computer. Intra-NBM injections of 2 or 10nmol of OT, but not 0.2nmol of OT, induced significant increase on the latency of spatial learning. Rats receiving intra-NBM administrations of 2 or 10nmol of OT showed a more random search pattern. There were no significant changes in the swimming speed in Morris water maze test after the injection of OT. Furthermore, the impaired effect of OT on the latency of spatial learning was blocked by intra-NBM injection of the selective OT antagonist Atosiban, indicating that the effect of OT was mediated by OT receptor in the NBM of rats. Moreover, there were no influences of OT or Atosiban on the retention performance in rats. The results suggest that OT plays an inhibitory role in spatial learning in the NBM; the effect is mediated by OT receptor.
Oxytocin, Nucleus basalis of Meynert, Spatial learning, Oxytocin receptor, Morris water maze
-
47浏览
-
0点赞
-
0收藏
-
0分享
-
191下载
-
0评论
-
引用
于龙川, Yan-Gang Sun, Long-Chuan Yu*
Regulatory Peptides 124(2005)37-43,-0001,():
-1年11月30日
The fact that galanin, β-endorphin and their receptors are present in the arcuate nucleus of hypothalamus (ARC), coupled with our previous observation that both β-endorphin and galanin play antinociceptive roles in pain modulation in the ARC, made it of interest to study their interactions. The hindpaw withdrawal latency (HWL) in response to noxious thermal and mechanical stimulation was assessed by the hot-plate test and the Randall Selitto Test. We showed that the antinociceptive effect induced by intra-ARC injection of galanin was dosedependently attenuated by the following intra-ARC injection of naloxone. Furthermore, intra-ARC administration of the selective μ-opioid receptor antagonist h-funaltrexamine (β-FNA) attenuated the increased HWL induced by intra-ARC injection of galanin in a dose-dependent manner, while the δ-opioid receptor antagonist naltrindole or the κ-opioid receptor antagonist nor-binaltorphimine (nor-BNI) did not. Moreover, intra-ARC injection of a galanin receptor antagonist galantide attenuated intraperitoneal morphine-induced increases in HWLs. These results demonstrate that the antinociceptive effect of galanin was related to the opioid system, especially A-opioid receptor was involved in, and that systemic morphine induced antinociception involves galanin in the ARC.
Antinociception, Galanin receptors, Galantide, Opioid receptors, Morphine
-
51浏览
-
0点赞
-
0收藏
-
0分享
-
173下载
-
0评论
-
引用
于龙川, Lian Gao, Long-Chuan Yu*
Regulatory Peptides 120(2004)53-58,-0001,():
-1年11月30日
Recent studies showed that oxytocin and opioid peptides play important roles in pain modulation at different levels in the central nervous system. The present study was performed to explore whether opioid system is involved in the oxytocin-induced antinociception in the brain of rats. The results showed that: (1) intracerebroventricular injection of oxytocin induced dose-dependent increases in hindpaw withdrawal latencies (HWL) to noxious thermal and mechanical stimulation in rats. (2) The antinociceptive effect of oxytocin was attenuated dosedependently by intracerebroventricular injection of naloxone, indicating an involvement of opioid system in the oxytocin-induced antinociception. (3) It is interesting that the antinociceptive effect of oxytocin was attenuated by subsequent intracerebroventricular injection of the μ-opioid antagonist β-funaltrexamine (β-FNA) and the κ-opioid antagonist nor-binaltorphimine (nor-BNI), but not the δ-opioid antagonist naltrindole. The results indicate that oxytocin plays an antinociceptive role in the brain of rats; μ- and κ-opioid receptors, not δ-receptors, are involved in the oxytocin-induced antinociception in the central nervous system of rats.
Antinociception, Oxytocin, μ-Opioid receptor, κ-Opioid receptor, Hindpaw withdrawal latency, Intracerebroventricular injection
-
65浏览
-
0点赞
-
0收藏
-
0分享
-
226下载
-
0评论
-
引用
于龙川, Yi Yan and Long-Chuan Yu*
Journal of Neuroscience Research 77: 148-152(2004),-0001,():
-1年11月30日
The present study was performed to explore the involvement of opioid receptors in the calcitonin gene-related peptide 8-37 (CGRP8-37, an antagonist of CGRP receptor)-induced inhibition of the activity of widedynamic-range (WDR) neurons in the spinal dorsal horn of rats. Extracellular recording was performed with a multibarrelled glass micropipette, and the chemicals were delivered by micro-iontophoresis. The discharge frequency of WDR neurons was evoked by subcutaneous electrical stimulation applied to the ipsilateral hindpaw. Iontophoretic application of CGRP8-37 by an ejection current of 160nA induced significant inhibition of the discharge frequency of WDR neurons. The inhibitory effect of CGRP8-37 on the activity of WDR neurons was attenuated by later iontophoretic application of the opioid antagonist naloxone. Furthermore, the effect of CGRP8-37 was attenuated by either iontophoretic application of the kappa-receptor antagonist norbinaltorphimine (nor-BNI) or the mu-receptor antagonist β-funaltrexamine (β-FNA) but not by the delta-receptor antagonist naltrindole. The results indicate that kappaand mu-opioid receptors on the membrane of WDR neurons are involved in the modulation of CGRP8-37-induced antinociception in dorsal horn of the spinal cord in rats.
calcitonin gene-related peptide (, CGRP), , wide-dynamic-range neurons, iontophoretic application, mu-opioid receptor, kappa-opioid receptor
-
40浏览
-
0点赞
-
0收藏
-
0分享
-
151下载
-
0评论
-
引用
于龙川, Yan-Gang Sun, , Ji Li, Bo-Ning Yang, and Long-Chuan Yu*
Journal of Neuroscience Research 77: 718-722(2004),-0001,():
-1年11月30日
Although the tuberomammillary nucleus (TM) is well defined in terms of anatomy and neurochemistry, little is known about its function in nociceptive modulation. There was an abundance of galanin-immunoreactive fibers in the TM, and galanin has been implicated in pain processing. The present study assessed the role of galanin in the modulation of nociception in the TM of rats. Intra-TM injection of galanin dose-dependently increased the hindpaw withdrawal latency of rats to a noxious thermal stimulus, indicating an antinociceptive role of galanin in the TM. The antinociceptive effect of galanin was blocked by a subsequent intra-TM injection of galantide, a putative galanin receptor antagonist, suggesting that the antinociceptive effect of galanin is mediated by galanin receptors. Moreover, there was abundant galanin receptor 1 (GalR1) in the TM, and the number of GalR1-positive neurons in the ipsilateral TM increased significantly after unilateral loose ligation of the sciatic nerve compared with the contralateral TM or the TM of intact rats. However, the number of GalR1-positive neurons was not significantly altered by carrageenaninduced inflammation, in either the ipsilateral or the contralateral TM. The results suggest that galanin and GalR1 in the TM may play important roles in pain regulation.
sciatic nerve chronic constriction injury, mononeuropathy, inflammation, pain modulation, thermal nociception testing
-
46浏览
-
0点赞
-
0收藏
-
0分享
-
144下载
-
0评论
-
引用
【期刊论文】Role of nociceptin in the modulation of nociception in the arcuate nucleus of rats
于龙川, Ning Li a, , Shi-Yi Wei a, Long-Chuan Yu a, Kumi Moriyama b, James Mitchell b, Pamela Pierce Palmer b, *
Brain Research 1025(2004)67-74,-0001,():
-1年11月30日
Neuropeptide nociceptin/orphanin FQ is the endogenous ligand for the opioid-receptor-like receptor 1 (ORL1), mediating essential functions in the central and peripheral nervous systems. The present study was performed to investigate the role of nociceptin and ORL1 receptor in nociception and morphine-induced antinociception in the arcuate nucleus of hypothalamus in rats. Hindpaw withdrawal latencies (HWL) were measured by hot-plate and Randall Selitto tests. The HWL to both thermal and mechanical stimulation decreased significantly after intra-arcuate nucleus injection of nociceptin in a dose-dependent manner. The effect of nociceptin was blocked significantly by subsequent intra-arcuate nucleus administration of [Nphe1] nociceptin (1-13)-NH2, an ORL1 receptor antagonist. Furthermore, an intra-arcuate nucleus injection of nociceptin dramatically attenuated the antinociceptive effect induced by morphine either injected in the same site or applied intraperitoneally. These results suggest that nociceptin in the arcuate nucleus induces a hyperalgesic effect by acting on ORL1 receptors. The present study also demonstrates an interaction between nociceptin and opioids in the arcuate nucleus of the hypothalamus.
Nociceptin/, orphanin FQ, ORL1 receptor, Opioid, Hyperalgesia, Hindpaw withdrawal, Hypothalamus
-
56浏览
-
0点赞
-
0收藏
-
0分享
-
120下载
-
0评论
-
引用
于龙川, Xi-Yun Cui a, b, Thomas Lundeberg c, Long-Chuan Yu a, *
Brain Research 995(2004)23-28,-0001,():
-1年11月30日
Corticotropin-releasing factor (CRF) plays important physiological functions in the central nervous system. The present study was performed to investigate the role of CRF and CRF receptor in nociceptive modulation in the central nucleus of amygdala (CeA) of rats. The hindpaw withdrawal latency (HWL) to noxious thermal and mechanical stimulation increased significantly after intra-CeA administration of 0.1 and 0.01nmol of CRF, but not 0.001nmol, indicating that CRF induces antinociceptive effects in the CeA of rats. The antinociceptive effect of CRF was attenuated by intra-CeA administration of 0.1nmolα-hCRF9-41, a selective CRF receptor antagonist, suggesting that the CRF-induced antinociception is mediated by the CRF receptors in the CeA. Furthermore, the HWL to both thermal and mechanical stimulation decreased significantly after intra-CeA administration of-αhCRF9-41 alone, suggesting an involvement of endogenous CRF in the CeA in nociceptive modulation. The present study demonstrated that both exogenous and endogenous CRF plays an antinociceptive effect in the CeA, the effect is mediated by CRF receptor.
Corticotropin-releasing factor (, CRF), , CRF receptor, Central nucleus of amygdala (, CeA), , Antinociception, Hyperalgesia
-
38浏览
-
0点赞
-
0收藏
-
0分享
-
83下载
-
0评论
-
引用
于龙川, Yan-Gang Sun a, Thomas Lundeberg b, c, Long-Chuan Yu a, b, *
Pain 104(2003)55-63,-0001,():
-1年11月30日
Although exogenous administration of beta-endorphin to the arcuate nucleus of hypothalamus (ARC) had been shown to produce antinociception, the role of endogenous beta-endorphin of the ARC in nociceptive processing has not been studied directly. The aim of the present study was to investigate the effect of endogenous beta-endorphin in the ARC on nociception in rats with carrageenan-induced inflammation. The hindpaw withdrawal latency (HWL) to noxious thermal and mechanical stimulation was assessed by the hot-plate test and the Randall Selitto Test. Intra-ARC injection of naloxone had no significant influence on the HWL to thermal and mechanical stimulation in intact rats. The HWL decreased significantly after intra-ARC injection of 1 or 10μg of naloxone in rats with inflammation, but not with 0.1μg of naloxone. Furthermore, intra-ARC administration of the selective mu-opioid receptor antagonist beta-funaltrexamine (beta-FNA) decreased the nociceptive response latencies to both stimulation in a dose-dependent manner in rats with inflammation, while intra-ARC administration of the selective delta-opioid receptor antagonist naltrindole or the selective kappa-opioid receptor antagonist norbinaltorphimine (nor-BNI) showed no influences on the nociceptive response latency. The antiserum against beta-endorphin, administered to the ARC, also dose-dependently reduced the HWL in rats with inflammation. The results indicate that endogenous beta-endorphin in the ARC plays an important role in the endogenous antinociceptive system in rats with inflammation, and that its effect is predominantly mediated by the mu-opioid receptor.
Arcuate nucleus, Inflammation, Beta-endorphin, Hyperalgesia, Beta-funaltrexamine, Mu-opioid receptor
-
33浏览
-
0点赞
-
0收藏
-
0分享
-
90下载
-
0评论
-
引用
于龙川, W. XU, a T. LUNDEBERG, b Y. T. WANG, a, c Y. LI a AND L.-C. YU a, b*
Neuroscience 118(2003)1015-1022,-0001,():
-1年11月30日
The central nucleus of amygdala (CeA) plays an important role in pain regulation. Calcitonin gene-related peptide (CGRP)-like immunoreactive fibers and CGRP receptors are distributed densely in CeA. The present study was performed to elucidate the role of CGRP in nociceptive regulation in the CeA of rats. Intra-CeA injection of CGRP induced dose-dependent increases in the hind-paw withdrawal latency tested by hotplate test and Randall Selitto Test, indicating an antinociceptive effect of CGRP in CeA. Furthermore, the antinociceptive effect of CGRP was blocked by intra-CeA administration of the CGRP receptor antagonist CGRP8-37, suggesting that CGRP receptor1 is involved in the CGRP-induced antinociception. The CGRP-induced antinociception was attenuated by s.c. injection of the opioid antagonist naloxone, suggesting an involvement of endogenous opioid systems in CGRP-induced antinociception. Moreover, it was demonstrated that opioid receptors in the periaqueductal gray, but not in CeA, contributed to the CGRP-induced antinociception, indicating the importance of the pathway between CeA and the periaqueductal gray in CGRP-induced antinociception. Combining retrograde fluorescent tracing with immunohistochemistry, we found that met-enkephalinergic neurons were innervated by CGRP-containing terminals in CeA. Furthermore, most neurons in the CeA retrogradely traced from the periaqueductal gray were contacted by CGRP-containing terminals and some of them were surrounded by characteristic basket-like structures formed by the terminals, suggesting that CGRP innervates the neurons which project from CeA to the periaqueductal gray. The results indicate that CGRP activates the met-enkephalinergic neurons, which project from CeA to the periaqueductal gray, producing antinociceptive effect in rats.
calcitonin gene-related peptide,, opioid receptors,, central nucleus of amygdala,, periaqueductal gray,, hind-paw
-
48浏览
-
0点赞
-
0收藏
-
0分享
-
91下载
-
0评论
-
引用
于龙川, Long-Chuan Yu a, b, *, Xing-He Weng a, Jing-Wen Wang a, Thomas Lundeberg b
Neuroscience Letters 349(2003)1-4,-0001,():
-1年11月30日
The present study investigated the role of calcitonin gene-related peptide (CGRP) in the modulation of nociception in periaqueductal grey (PAG) of rats. Hindpaw withdrawal latencies (HWLs) were tested by hot-plate and Randall Selitto tests. The HWLs to thermal and mechanical stimulation increased significantly after intra-PAG administration of 0.26 or 0.13nmol of CGRP, but not 0.026nmol of CGRP. The anti-nociceptive effects induced by CGRP were significantly blocked by intra-PAG administration of 0.026 or 0.26nmol of the CGRP1 receptor antagonist CGRP8-37. Furthermore, administration of CGRP into the decussation of superior cerebellar peduncle, out of PAG, did not elicit anti-nociceptive effects during 60min after the injection. The results demonstrated that CGRP plays an important role in antinociception in PAG of rats, and CGRP1 receptor is involved in the CGRP-induced anti-nociception.
Calcitonin gene-related peptide, CGRP8-37, CGRP1 receptor, Periaqueductal grey, Hindpaw withdrawal latency, Anti-nociception
-
81浏览
-
0点赞
-
0收藏
-
0分享
-
116下载
-
0评论
-
引用