李成章
牙周病的宿主因素与引导牙周组织再生
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- 姓名:李成章
- 目前身份:
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学术头衔:
博士生导师
- 职称:-
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学科领域:
口腔医学
- 研究兴趣:牙周病的宿主因素与引导牙周组织再生
李成章、男、武汉大学口腔医学院教授,牙周黏膜科主任、教研室主任。研究专业:牙周病学 研究领域:牙周病的宿主因素与引导牙周组织再生 电子信箱:lcz56@163.com 更多信息:医学微生物学与免疫学硕士、医学博士。硕士生导师、博士生导师。从事牙周病防治的基础研究和临床工作,发表论著50余篇,参编专著和卫生部统编教材11部,获鉴定成果4项,其中3项分获省级科技进步二等奖、三等奖和省级教学成果一等奖。获发明专利一项。湖北省有突出贡献的中青年专家、湖北省跨世纪学科带头人。 兼职情况 中华口腔医学会老年口腔医学专业委员会常委,中华口腔医学会牙周病学专业委员会委员,中华口腔医学会口腔保健用品专家评审委员会委员, 湖北省口腔医学会理事,《口腔医学纵横》杂志编委。 教学经历:承担医疗系、口腔系本科生的医学微生物学与免疫学教学;本科生、研究生牙周病学教学;协助樊明文教授开展新兴学科“口腔生物学”的教学, 并发表“教学模式与国际接轨的探索与实践”等教学论文,该论文获湖北省高等教育科研论文三等奖;开展“牙周病学”PBL教学并建立其评估方法。 科研情况: 已完成的科研项目: 成果鉴定及获奖 1. 厌氧螺旋体的培养条件研究及与青少年牙周炎感染的关系 国内领先 2. 双抗胶原膜的研制及临床初步应用 国际领先 97省科技进步二等奖 3. 口腔医学教学建设的探索与实践 国际先进 97省教学成果一等奖 目前进行的科研项目: 国家自然科学基金项目:EMMPRIN/MMPs信号通路在牙周炎组织降解过程中的调控机制研究武汉市科技攻关项目:口腔癌及癌前病变鉴别和诊断试剂盒湖北省科委项目:天然药物调控MMPs/TIMP防治牙周炎的研究 本专业的研究进展及研究生培养计划: 依研究方向培养科学学位和专业学位研究生1—2名/年 擅治疾病: 牙周病 学术荣誉和荣誉称号 湖北省有突出贡献的中青年专家。湖北省跨世纪学科带头人。 学术活动举办湖北省首期牙周病学继续教育学习班 (2004)第五届IADR会议中国分会 (武汉 2004) 82届IADR会议 (美国 2004) 主要学术著作、论文 《美容牙科学》 人民卫生出版社 2002. 现代口腔医学》(上、下册) 科学出版社 2003 《口腔生物学》第1,2版 人民卫生出版社 2004. 黄芩苷对牙龈成纤维细胞和牙周膜细胞pro-MMP-1/MMP-3 表达的影响研究 中华口腔医学杂志 2004,39(3) 双抗胶原膜引导牙周组织再生 武汉大学学报(医学版) 2003,24(2) 双抗胶原膜的研制及特性分析 中华口腔医学杂志 1997,(6) 指导研究生情况: 指导已毕业、在读硕士15人,已毕业、在读博士5人。
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李成章, 尚姝环, 樊明文
武汉大学学报(医学版),2003,24(2):180-182,-0001,():
-1年11月30日
目的:将牛腱胶原膜加工制备成双抗胶原膜应用于临床GTR治疗,观察比较牛腱胶原膜和双抗胶原膜的临床疗效。方法:随机分组,分别应用双抗胶原膜和牛腱胶原膜对24例牙周炎患者,共26颗患牙、31处骨病损进行GTR治疗,术后6个月复查检测临床指标及Digora分析平行定位X线片骨密度的改变。结果:实验组(双抗胶原膜组)临床附着获得平均为(2.216±1.184)mm;对照组(牛腱胶原膜组)平均为(1.316±0.719)mm(t2test P<0.05);Digora分析显示GTR术后实验组骨密度增加值平均为(25.71±12.25),对照组为(11.29±12.73)(t2test P<0.05)。结论:临床应用双抗胶原膜比应用牛腱胶原膜能获得更多的生理性新附着,更大程度地促进牙槽骨生理性再生。提示加工改良牛腱胶原膜为双抗胶原膜具有较大的临床应用前景。
引导组织再生, 胶原膜, 牙周炎
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李成章, 樊明文
中华口腔医学杂志,1997,32(6):369-371,-0001,():
-1年11月30日
为制备具有抗菌斑和抗胶原酶特性的引导牙周组织再生(GTR)胶原膜,作者将普通交联胶原膜用四环素(TC)缓释装置包被,制成双抗胶原膜(BACM)。检测结果:BACM具均匀小网孔;低应变区弹性模量和膨胀率分别为2014g/mm2 和01141;豚鼠过敏试验阴性;抗100u胶原酶消化时达30d以上;1 mg BACM植入小鼠皮下可维持7w左右;BACM(含TC 150μg)置入牙周袋内7d,龈沟液TC浓度维持在46176μmol/L±5.69μmol/L;植入翻瓣术患者根面7d,其上附着菌明显少于对照膜。结果提示具有双抗效应的BACM用于GTR将发挥更好的作用。
引导组织再生, 胶原, 四环素, 牙周炎
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【期刊论文】人牙骨质、牙周膜、牙槽骨中Ⅰ、Ⅱ、Ⅲ型胶原的检测
李成章, 樊明文, 唐志姣
中华口腔医学杂志,1997,32(2):70-72,-0001,():
-1年11月30日
本研究目的为检测人牙骨质、牙周膜和牙槽骨组织中Ⅰ、Ⅱ、Ⅲ型胶原的分布和含量。应用免疫组化和图像分析法测得:Ⅰ型胶原占牙周膜的78.06%、牙骨质的73.09%和牙槽骨的30.50%;Ⅲ型胶原占牙周膜的11.73%,在根尖部牙骨质和相对应的牙槽窝骨组织也观察到Ⅲ型胶原;Ⅳ型胶原仅见于血管基膜;夏柏纤维为Ⅰ型着色,但周边显示有Ⅰ型胶原和Ⅲ型胶原的“鞘”状着色。结果显示了人正常牙周组织的胶原状况,将有助于进一步对牙周组织病理过程和再生效应的研究。
胶原, 牙骨质, 牙周膜, 牙槽骨, 免疫组织化学
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【期刊论文】黄芩苷对牙龄成纤维细胞和牙周期膜细胞Pro-MMP-1和MMP-3表达的影响
李成章, 曹正国, 杨茹, 尚姝环, 金力坚, Cobert EF
中华口腔医学杂志,2004,39(3):197-200,-0001,():
-1年11月30日
目的:检测黄岑苷对白细胞介素1β(IL-1β)诱导作用下人牙龄成纤维细胞(HGF)分泌基质蛋白酶1酶原(pro-MMP-1)的量和牙周膜细胞(PDLCs)基质金属蛋白酶3(MMP-3)表达的变化. 方法:体外培养HGF和PDLCs,分别运用ELISA和免疫组化方法pro-MMP-1的量和MMP-3的表达. 结果:与对照组的(1.960±0.176)μg/L的IL-1β能够显著促进HGF分泌pro-MMP-1(3.333±0.123)μg/L,且增中PDLCsMMP-3的表达(P<0.001);加入黄芩苷后能降HGF的pro-MMP-1分泌量,其作用呈浓度(10-1000μg/L)依赖性;黄芩苷对IL-1β诱导下PDLCs合成MMP-3的能力没有影响,但是能够抑制MMP-3的释放. 结论:黄芩苷能够抑制由IL-1β介导的HGF分泌pro-MMP-1和DLCsMMP-3的表达,提示黄芩苷可用于牙周病的防治。
黄芩甙, 基质金属蛋白酶, 成纤维细胞
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李成章, Z. Cao, , C. Li & G. Zhu
Journal compilation 68(38-43)2006 Blackwell Munksgaard,-0001,():
-1年11月30日
A single nucleotide polymorphism in the promoter region of -1607 bp of the human MMP-1 gene has been found to be associated with an increased risk of various inflammatory diseases and cancer metastasis. This study aimed to evaluate the association between the MMP-1 promoter gene polymorphism and chronic periodontitis susceptibility and/or severity in a Chinese population. Genomic DNA was obtained from whole blood samples in 60 Chinese subjects with chronic periodontitis and 50 periodontally healthy subjects as controls. MMP-1 promoter fragment was amplified by polymerase chain reaction, and the polymorphism was analyzed by restriction endonuclease cleavage. In the control subjects, the 2G allele was observed a frequency of 49%, while in severely diseased patients, the 2G allele was seen in 73.4%. The individuals with the 2G allele seem to be approximately three times at greater risk for developing the severe chronic periodontitis (w2
Chinese, genotypes, MMP-1, periodontitis, polymorphism
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李成章, Zheng-Guo Cao a, Cheng-Zhang Li b, *
Oral Oncology (2006) 42, 32-38,-0001,():
-1年11月30日
We genotyped 96 oral squamous cell carcinoma (OSCC) patients for the 1G/2G polymorphism of matrix metalloproteinase-1 (MMP-1) promoter -1607bp using PCR-RFLP. A control population of 120 frequency-matched subjects was also genotyped for the same polymorphism.The detection frequency of 2G allele was significantly higher in OSCC subjects (76%) than in the control group (56.7%). The frequency of 2G allele had a significant difference between the OSCC and controls group (p=0.00, Odds Ratio, OR=2.232, 95% CI=1.477-3.372). The genotype 2G/2G was found in 57.3% of the OSCC, and 34.2% in the controls. The proportion of 2G homozygote (2G/2G) was significantly higher in the OSCC group when compared to controls (p=0.001, OR=2.585, 95% CI=1.487-4.494). OSCC patients were stratified by clinicopathological parameters including gender, smoking, clinical stage and lymph node metastasis, but the only statistically significant association with MMP-1 genotype was with smoking. The results showed that a SNP in the MMP-1 promoter-1607bp was associated with OSCC susceptibility in a Chinese population.
Oral squamous cell carcinoma, Matrix metalloproteinase-1, Polymorphism, Gene
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李成章, Zhengguo Cao, Chengzhang Li, Lijian Jin, Esmonde F. Corbet
J Periodont Res 2005; 40; 427-431,-0001,():
-1年11月30日
Objectives: The present study aimed to examine the distribution of MMP-1 genotypes in a group of Chinese subjects with generalized aggressive periodontitis and a group of periodontally healthy subjects, and to evaluate the possible association of the MMP-1 promoter polymorphism with aggressive periodontitis. Methods: Genomic DNA was obtained from whole blood samples in 40 Chinese subjects with generalized aggressive periodontitis and 52 periodontally healthy subjects as controls. MMP-1 promoter fragment was amplified by polymerase chain reaction, and the polymorphisms were analyzed by restriction endonuclease cleavage. The alleles were detected by polyacrylamide gel electrophoresis and visualized with ethidium bromide. Results: The detection frequency of 2G allele was significantly higher in the subjects with generalized aggressive periodontitis (68.7%) than in the control subjects (49%) (p < 0.01). The genotype of 2G/2G was found in 52.5% of the patients, which was significantly greater than that of control subjects (23.1%) (p < 0.05). Conclusion: The present study suggests that a single nucleotide polymorphism in the MMP-1 promoter region of )1607 bp may be associated with generalized aggressive periodontitis in Chinese population.
aggressive periodontitis, genotypes, matrix metalloproteinase-1, polymorphism
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李成章, Zhengguo Cao, DDS, MS*, Chengzhang Li, PhD†, Xianqun Wu**
,-0001,():
-1年11月30日
Objective: The aim of this study was to clarify the effects of baicalin on the biological properties of human periodontal ligament cells. Materials and Methods: Effects of baicalin on proliferation, alkaline phosphatase activity (ALPase activity), the synthesis of the total protein and ultrastructure of human periodontal ligament cells were examined in this study. Culturing PDL cells in vitro, MTT assay, coomasie brilliant blue staining, ALPase kit and transmission electron microscope were involved in this research. Results: The range of 0.01~100ng/ml baicalin enhanced the proliferative response of PDL cells. The maximum mitogenic effect of baicalin on PDL cells was observed at the concentration of 10ng/ml. The proliferative response was minimal at 1 d, but marked at 3 d and 5d. The range of 1~1000ng/ml baicalin may enhance the synthesis of the total protein of PDL cells, which also marked at 3 d and 5d and the response has a dose-dependent and time-dependent manner in the range of 1~100ng/ml baicalin. The optimal effect of baicalin was observed at the concentration of 100ng/ml, when more than 100ng/ml of baicalin was added to the culture, the total protein of PDL cells decreased. 10~100ng/ml baicalin could increase the ALPase activity. Moreover, 10ng/ml baicalin could increase the number of mitochondria and rough endoplasmic reticulum of PDL cells. Conclusion: The results showed that baicalin could increase the proliferation and the synthesis of the total protein of PDL cells, which suggested that it might play a role in accelerating periodontal tissue regeneration.
Baicalin, PDL cells, Proliferation, ALPase
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