黄承志
个性化签名
- 姓名:黄承志
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学术头衔:
博士生导师, 国家杰出青年科学基金获得者
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学科领域:
分析化学
- 研究兴趣:
黄承志, 男, 1965年国庆出生在四川省富顺县, 西南大学教授、博士生导师。国家杰出青年科学基金获得者,教育部第五届科学技术委员会化学化工部学部委员(2004-2007)、重庆市首届学术技术带头人(2002年),《西南师范大学学报(自然科学版)》和《现代仪器》杂志编委。2004年获国务院特殊津贴和教育部表彰的“全国优秀教师”,重庆市青年科技奖创新奖; 2002年获教育部“2002年度提名国家科学技术奖”自然科学奖一等奖和“全国高等学校优秀骨干教师”称号、2001年获“首届重庆市青年科技创新奖杰出奖”、1999年获“中国化学会青年化学奖”。
1986.7本科毕业于西南师范大学化学系, 毕业后留校工作4年, 随后考入北京师范大学化学系, 攻读分析化学专业硕士学位,导师迟锡增教授。1993.9毕业后考入北京大学化学与分子工程学院攻读分析化学专业博士学位, 导师童沈阳教授。1996, 7获博士学位后回到西南师范大学工作, 任副教授。1998.7破格晋升为教授, 2000年任分析化学专业博士生导师。1998.11-1999.10在日本日立中央研究所分析生化高级访问研究员, 2000.12-2001.05在加拿大渥太华大学生物化学系细胞生物学博士后, 2002.08-2003.07在日本东京大学大学院药学研究科生体与药物分析学博士后。
目前正主持国家杰出青年基金一项、国家自然科学基金面上项目2项,教育部新世纪人才计划项目一项。已主持完成了国家自然科学基金项目4项,教育部人才基金项目3项,重庆市科委应用基础研究项目4项。1998年以来,先后在西班牙第8界国际发光学术会议, 日本药学会122年会、123年会、欧洲第13届分析化学年会、第五届、第六届中日韩环境分析化学学术年会和国内学术年会上作报告。并应邀为《分析化学学报(欧洲)》撰写综述。
到目前为止,在《分析化学(美)》、《分析家(英国)》和《中国科学》(B)等国内外知名学术刊物上发表学术论文120篇,其中被SCI和SCIE收录的有90篇, SCI和SCIE收录刊物他引数达到700次。据国家自然科学基金委和中国科学院情报中心统计,1996和1997年在《分析化学(美)》上发表的两篇论文的SCI收录刊物单篇他引次数在全国1994年以来发表的论文中排名第二位和第三位。据中国科学院文献情报研究中心统计,2001年其发表的论文引用次数在全国排名第七。
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19
黄承志, Ping Feng, † Wei Qun Shu, ‡ Cheng Zhi Huang, *, † and Yuan Fang Li †
Anal. Chem. 2001, 73, 4307-4312,-0001,():
-1年11月30日
A highly selective method of chlortetracycline (CTC) is proposed on the basis of the measurements of total internal reflected resonance light scattering (TIR-RLS) at water/tetrachloromethane (H2O/CCl4) interfaces. In the pH range of 7.54-8.14, the interaction of the binary complex of Eu (III)/CTC in the presence of trioctyl phosphine oxide (TOPO) occurs at the H2O/CCl4 interface, resulting in greatly enhanced TIR-RLS signals with the maximum peak located at 340nm. The enhanced TIRRLS intensity is in proportion to the CTC concentration in the range 0.98~20.0×10-7 mol/L. The limit of detection is 9.8×10-9 mol/L. Synthetic samples and body fluid samples including human urine, human serum, and fresh milk were determined with the recovery of 95.4-106.4% and RSD of 2.9-3.9%.
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黄承志, Cheng Zhi Huang, * Yuan Fang Li, Xin Hua Huang and Ming Li
Analyst, 2000, 125, 1267-1272,-0001,():
-1年11月30日
A novel assay of DNA with a sensitivity at the nanogram level is proposed based on the measurement of enhanced resonance light scattering (RLS) signals resulting from the interaction of Janus Green B (JGB) with DNA. At pH 6.37 and ionic strength<0.20, the RLS signals of JGB were greatly enhanced by DNA in the region of 300-650nm characterized by three peaks at 416.0, 452.0 and 469.2nm. The binding properties were examined using a Scatchard plot based on the measurement of the enhanced RLS data at 416.0nm at a high JGB+DNA molar ratio (R>2.22), and an aggregation mechanism of JGB in the presence of DNA at the nanogram level is proposed. Linear relationships can be established between the enhanced RLS intensity and DNA concentration in the range 0-3.5mg ml21 for both calf thymus DNA (ctDNA) and fish sperm DNA (fsDNA) if 2.0
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黄承志, Cheng Zhi Huang*, Yuan Fang Li, Xiao Li Hu, Nian Bin Li
Analytica Chimica Acta 395(1999)187-197,-0001,():
-1年11月30日
The features of the three-dimensional (3-D) emission spectrum of the long range assembly of Nile Blue sulfate (NBS) on the molecular surfaces of DNAs were discussed. It was found that the emission signals involve resonance light-scattering (RLS, λRLS=λex), second order light-scattering (SLS, λSLS=2λex), anti-second order light-scattering (ASLS, λASLS=0.5λex), Raman light-scattering (Raman,λRLSR=-49.0+1.28λex, λSLSR=-52.0+0.64λex, and λASLSR=-171.7+2.86λex), and fluorescence (λex=545.0nm, λem=610.0nm). The wavelengths of all light-scattering signals keep linear relationships with at of the incident light beam (λex), and the intensities of the light-scattering signals in the 3-D spectrum are in the order: IRLS>ISLS>IASLS>IRLSR>ISLSR>IASLSR. At pH 7.20-7.80 and ionic strength 0.012, these signals, including RLS, SLS, and ASLA, were found to be strongly enhanced because of the long range assembly of NBS on the molecular surface of both calf thymus DNA (ctDNA) and Æsh sperm DNA (fsDNA). Fluorescence quenching of NBS by DNAs occurs, but no signiÆcantly enhanced Raman light-scattering signals of NBS can be detected in the long range assembly. By independently using the enhanced intensity of RLS at 293.8nm (λex=293.8nm), ASLS intensity at 293.8nm (λex=587.6nm), or SLS intensity at 587.6nm (λex=293.8nm), ctDNA and fsDNA at nanogram levels can be determined with identical results.
Three-dimensional spectrum, DNA, Nile Blue sulfate (, NBS), , Light-scattering
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黄承志, Cheng Zhi Huang*, Yuan Fang Li, Dao Jian Zhang, Xiao Ping Ao
Talanta 49(1999)495-503,-0001,():
-1年11月30日
The supramolecular interaction of nile blue sulphate (NBS) with nucleic acids was studied by investigating the characteristics of the interaction absorption spectra on the basis of the drug binding process in organic system in which small amount of drug interacting with large amount of biological macromolecules involves, and an accordingly binding model for organic dyes with large amount of macromolecules was established. At pH 7.40 and ionic strength 0.004, the H-aggregation of NBS occurs with increasing NBS concentration. The NBS aggregates can be bound to both calf thymus DNA and fish sperm DNA by the ratio of each nucleotide residue with a molecule of NBS if the concentration of DNAs is more than 15-fold excessive. The corresponding binding constant for the interaction of NBS with DNAs is about 103 order, with which thermodynamic parameters for the interactions, such as the change of free energy, enthalpy and entropy at 25℃, were calculated. It was found that the binding of NBS with thermally denatured DNA is similar to that with native yeast RNA, which indicates H-aggregation of NBS can be encouraged by single stranded nucleic acids.
Nucleic acids, Nile blue sulphate, Absorption spectrum
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黄承志, Cheng Zhi Huang*, Yuan Fang Li, Jian Guo Mao and Dai Gao Tan
Analyst, June 1998, Vol. 123(1401-1406),-0001,():
-1年11月30日
A method of protein determination with the limit of determination at nanogram levels is proposed by using a common spectrofluorometer to detect the intensity of preresonance light-scattering (PRLS). In the pH range 1.81-4.10, the interactions of α,β,γ,δ-tetrakis (5-sulfothienyl)-porphine, T (5-ST) P, with proteins were studied. It was found that the interactions result in a strongly enhanced preresonance light-scattering signal at 472.0nm. Mechanism studies showed that the enhanced preresonance light-scattering stems from the J-aggregation of T (5-ST) P in the presence of proteins. It was found that the J-aggregation process is speedy and is scarcely affected by temperature, which supplies a precise method for the determination of proteins. Different proteins in the range 0-7mg ml21 can be determined with the limits of determination below 100ng ml21 depending on the concentration of T (5-ST) P. The results of determination for synthetic samples were in agreement with the desired values, and the ones for human serum samples were identical to those obtained according to the Bradford method using CBB G-250.
Proteins, α,, β,, γ,, δ-tetrakis (, 5-sulfothienyl), porphine, preresonance light-scattering, J-Aggregation
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黄承志, Cheng Zhi Huang a, *, Yuan Fang Li a, Xi Dong Liu b
Analytica Chimica Acta 375(1998)89-97,-0001,():
-1年11月30日
This is the
Calf thymus DNA, Fish sperm DNA, Yeast RNA, Safranine T, Resonance light-scattering (, RLS),
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黄承志, Cheng Zhi Huang , Ke An Li, Shen Yang Tong*
Analytica Chimica Acta 345(1997)235-242,-0001,():
-1年11月30日
A new ternary azo-dye complex reaction involving nucleic acids has becn studied to provide a sensitive spectrophotometric method for nucleic acids. In alkaline conditions. Single-stranded nucleic acids teact with the complex of (II) with 4-[ (5-chloro-2-pyridyl) azo]-1, 3-diaminobcnzene (5-Cl-PADAB), resulting in purple ternary compounds with maximum absorbance at 545.0nm against the binary complex blank. The mole ratio of Co (II) to 5-Cl-PADAB in the ternary compounds is 1:2, and the concentrations of the tested nuclcic acids, such as calf thymus DNA is, 40ng ml. the relative standard deviation at 3.0ug ml-1 is 0.79% (m=10) and the Sandell's sensitivity is 5.3ng cm2. The same parameters for fish sperm DNA are 41ng ml-1, 0.84% and 5.4ng cm-2, and for yeast RNA are 49ng ml-1, 0.58% and 6.2ng cm-2. Synthetic samples were analyszed satisfactonrily.
Caaif thymus DNA, Fish sperm DNA, Yeast RNA, Cobalt (, II), : 4-[(, 5-chloro-2-pyridy), azo]-1,, 3-diaminobenzene (, 5-Cl-PADAB), , Spectrophotomctry
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黄承志, Cheng Zhi Huang, † Ke An Li, * and Shen Yang Tong
Anal. Chem.1997, 69, 514-520,-0001,():
-1年11月30日
Using a common spectrofluorometer to measure the intensity of resonance light-scattering, a method for determination of nucleic acids in the nanogram range has been developed. In the pH range 11.5-12.0, the resonance light-scattering of the binary comlpex of cobalt (II)/4-[(5-chloro-2-pyridyl) azo]-1,3-diaminobenzene (5-Cl-PADAB) is greatly enhanced by nucleic acids, with the maximum scattering peak located at 547.0nm. The enhanced intensity of resonance light-scattering is in proportion to the concentration of calf thymus DNA in the range 0-400ng/mL and to that of fish sperm DNA and yeast RNA in the range 0-300ng/mL. The limits of detection are 1.4ng/mL for calf thymus DNA, 0.8ng/mL for fish sperm DNA, and 1.3ng/mL for yeast RNA. Precision at 200ng/mL for the three nucleic acids is 1.9%, 2.0%, and 0.8%, respectively. Six synthetic samples were determined satisfactorily. Mechanism studies showed that the nature of the reaction is that the binary complex of Co (II)/5-Cl-PADAB reacts with single-stranded nucleic acid, and the enhancement effect of nucleic acids on the resonance light scattering of the binary complex is due to the stacking of the binary complex on nucleic acids, which act as a template.
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黄承志, Cheng Zhi Huang*, Ping Feng, Yuan Fang Li, Ke Jun Tan, Hui Ying Wang
Analytica Chimica Acta 538(2005)337-343,-0001,():
-1年11月30日
In aqueous medium of pH 5.33, penicillin, such as ampicillin (AmP), benzyl penicillin (BP), oxacillin (OA) and amoxycillin (AmO), interacts with berberine (BB), forming ion associates through electrostatic attraction of the polar head groups of penicillin and BB. The formed ion associates are then adsorbed into the amphiphilic H2O/CCl4 interfaces, displaying greatly enhanced total internal-reflected resonance light scattering (TIR-RLS) signals with the maximum peak at 370nm. With the enhanced TIR-RLS data, thermodynamic parameters of adsorption of penicillin-BB ion associates into interfaces were measured. Mechanism studies show that the ion associates of penicillin with BB at H2O/CCl4 interfaces have 1:1 binding molar ratio, and the formation constants are in the range of 3.67×104 to 5.48×104mol−1 dm4. It was found that the enhanced TIR-RLS intensity was directly proportional to the concentration of penicillin in the range of 0.98×10−7 to 10.0×10−7mol l−1 for BP, 0.87×10−7 to 10.0×10−7mol l−1 for OA, 1.1×10−7 to 12.0×10−7mol l−1 for AmP and 1.2×10−7 to 12.0×10−7mol l−1 for AmO with the corresponding limits of determination (3σ) being 3.48×10−8, 3.85×10−8, 4.22×10−8 and 4.86×10−8mol l−1, respectively. © 2005 Elsevier B. V. All rights reserved.
Water/, tetrachloromethane interface, Total internal-reflected resonance light scattering, Penicillin, Berberine chloride
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黄承志, Cheng Zhi Huang, a, * Ying Liu, b Yong Hong Wang, a and Hong Ping Guo a
Analytical Biochemistry 321(2003)236-243,-0001,():
-1年11月30日
A resonance light scattering (RLS) imaging technique was introduced to measure the light scattering of aggregation species induced by proteins, and thus a method of detecting proteins in the range of picograms was proposed. In acidic medium, J-aggregation of α,β,γ,δ-tetrakis(p-sulfophenyl) porphyrin (TPPS4) in the presence of proteins occurs, resulting in strong RLS signals characterized at 490nm. Under the excitation of a 488-nm light beam of argon ion laser source, the scattered light of single Jaggregation species could be observed with a common microscope, and the images could be captured with a cooled charge-coupled device camera. Data analysis for the digital images showed that the counts of aggregate species in the detection focus plane are proportional to the concentration of proteins in picograms. When 1.0×10ˉ7M TPPS4 was employed, 0.01210 ng/ml bovine serumalbumin and human serum albumin could be detected with limits of detection lower than 10 pg/ml (3r). Three human blood serumsamples were satisfactorily detected with relative standard deviations lower than 3.04%.
Proteinα,, β,, γ,, δ-Tetrakis (, p-sulfophenyl), porphyrin, Resonance light scattering imaging technique, Laser
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