宋凤鸣
主要从事植物抗病性的分子机理及其调控等研究。
个性化签名
- 姓名:宋凤鸣
- 目前身份:
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学术头衔:
博士生导师, 优秀教师/优秀教育工作者
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学科领域:
海洋化学
- 研究兴趣:主要从事植物抗病性的分子机理及其调控等研究。
宋凤鸣,男,1965年10月生。博士,教授,博士生导师。1987年浙江农业大学植保专业本科毕业;1990年浙江农业大学植物病理学硕士研究生毕业,农学硕士;2000年7月浙江大学植物病理学博士研究生毕业,农学博士。1990~1993年,浙江农业大学植保系助教;1994~1997年,浙江农业大学植保系讲师;1998~2002年,浙江大学农学院植保系副教授;2003.01~至今,浙江大学农学院植物病理学教授、博士生导师。1998年1月~2000年4月,美国University of Wisconsin植物病理系访问学者;2005年1~12月,美国普渡大学植物学和植物病理学系访问学者。2004年入选浙江省“151人才工程”第二层次,2005年入选教育部新世纪优秀人才支持计划。主要从事植物抗病性的分子机理及其调控等研究,先后主持国家自然科学基金项目“自由基和膜脂过氧化在水稻与病原物互作中的作用”、“水稻—白叶枯病菌互作中质膜组分和活性氧积累的关系”、“水稻系统获得抗病性中MAPK信号传导途径组分的克隆鉴定”、“高通量克隆鉴定与水稻抗病反应有关的转录因子”和“一个水稻新型转录因子OsCAMTA1的生化与生物学功能分析”、教育部新世纪优秀人才资助计划项目“水稻抗病性相关新基因在抗病信号途径中的功能分析”、教育部重点项目“水稻诱导抗病性相关新基因在抗病信号途径中的功能鉴定”以及教育部优秀青年教师资助计划项目“水稻诱导抗病性相关基因的功能分析”等的研究工作。在国内外核心期刊上发表论文50余篇,其中在Molecular Plant-Microbe Interaction、Planta、Gene、Journal of Experimental Botany、Plant Biology、Physiological and Molecular Plant Pathology、Molecular Biology Reports等国际期刊上发表SCI论文8篇。
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宋凤鸣, FENGMING SONG* and ROBERT M. GOODMAN†
Physiological and Molecular Plant Pathology (2002) 61, 31-40,-0001,():
-1年11月30日
Phospholipid signaling is an important component in eukaryotic signal transduction pathways; in plants, it plays a key role in growth and development as well as in responses to environmental stress. We cloned and characterized a gene from rice encoding phosphoinositide-specific phospholipase C (OsPI-PLC1, Oryza sativa L. phosphoinositide-specific phospholipase C1). OsPI-PLC1 encodes a 599 amino acid protein containing the catalytic X and Y domains, as well as a C2-1ike domain, characteristics of this class of enzymes. Expression of OsPI-PLC1 was induced by various chemical and biological inducers of plant defence pathways, including benzothiadiazole (BTH), salicylic acid (SA), dichloroisonicotinic acid, probenazole (PB), jasmonic acid (JA) and its methyl ester, Pseudomonas syringae pv. syringae, and wounding. All of these treatments were shown to induce resistance in rice against blast disease caused by Magrzaporthe grisea. OsPI-PLC1 was activated within 6 h after inoculation with the blast fungus in BTH-treated rice seedlings and in the incompatible interaction between a resistant genotype of rice and M. grisea, whereas the expression in the BTH-untreated seedlings and in the compatible interaction was only induced to a relatively low level at later time points (30h) after inoculation, indicating that expression of OsPI-PLC1 is associated with the resistance response and/or the incompatible interaction. In addition, BTH treatment also induced systemic expression of OsPI-PLC1. These results suggest that OsPI-PLC1 may be involved in the signaling pathways leading to disease resistance in rice.
benzothiadiazole, Magnaporthe grisea, phosphoinositide-specific phospholipase C (, PI-PLC), , rice (, Oryza sativa L., ), , OsPI-PLC1, systemic acquired resistance.,
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宋凤鸣, H. Luo, , F. Song, R. M. Goodman, and Z. Zheng
Plant Biol. 7 (2005): 459-468,-0001,():
-1年11月30日
In the present study, we cloned and identified a fulllength cDNA of a rice gene, OsBIHD1, encoding a homeodomain type transcriptional factor. OsBIHD1 is predicted to encode a 642 amino acid protein and the deduced protein sequence of OsBIHD1 contains all conserved domains, a homeodomain, a BELL domain, a SKY box, and a VSLTLGL box, which are characteristics of the BELL type homedomain proteins. The recombinant OsBIHD1 protein expressed in Escherichia coil bound to the TGTCA motif that is the characteristic c/s-element D NA sequence of the homeodomain transcriptional factors. Subcellular localization analysis revealed that the OsBIHD1 protein localized in the nucleus of the plant cells. The OsBIHD1 gene was mapped to chromosome 3 of the rice genome and is a single-copy gene with four exons and three introns. Northern blot analysis showed that expression of OsBIHD1 was activated upon treatment with benzothiadiazole (BTH), which is capable of inducing disease resistance. Expression of OsBIHD1 was also up-regulated rapidly during the first 6 h after inoculation with Magnaporthe grisea in BTH-treated rice seedlings and during the incompatible interaction between M. grisea and a resistant genotype. These results suggest that OsBIHD1 is a BELL type of homeodomain transcription factor present in the nucleus, whose induction is associated with resistance response in rice.
Benzothiadiazole (, BTH), , rice (, Oryza sativa L), , homeodomain, disease resistance response, OsBIHD1, Magnaporthe grisea.,
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宋凤鸣, Hongli Luo, , *, Fengming Song, †, and Zhong Zheng
Journal of Experimental Botany, Vol. 56, No.420, pp. 2673~2682, October 2005,-0001,():
-1年11月30日
The rice OsBIHD1 gene encodes a transcriptional factor belonging to the homeodomain class. It had previously been shown to be activated by treatment with benzothiadiazole, a chemical inducer of disease resistance, and in an incompatible interaction between rice and the blast fungus. To allow a better understanding of the function of OsBIHD1 in plant disease resistance response, the OsBIHD1 gene in tobacco was overexpressed by Agrobacterium-mediated leaf disc transformation with a construct containing the OsBIHD1 ORF under control of the 35S promoter. Overexpression of the rice OsBIHD1 gene in some of the transgenic tobacco lines led to some morphological abnormalities in the top buds and roots. The transgenic tobacco plants showed an elevated level of defence-related PR-1 gene expression and enhanced disease resistance against infection by tomato mosaic virus, tobacco mosaic virus, and Phytophthora parasitica var. nicotianae. However, the transgenic tobacco plants overexpressing OsBIHD1 showed enhanced sensitivity to salt and oxidative stress as compared with the wild-type plants. The results suggested that the OsBIHD1 protein may be positively involved in activating expression of the defence-related genes in disease resistance responses, and is also important in rice development and abiotic stress tolerance.
Disease resistance response, homeodomain, OsBIHD1, oxidative stress tolerance, salt tolerance,, transgenic tobacco plant
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【期刊论文】OsBIMK1, a rice MAP kinase gene involved in disease resistance responses
宋凤鸣, Fengming Song
Planta (2002) 215: 997-1005,-0001,():
-1年11月30日
The activation of mitogen-activated protein kinases (MAPKs) has been previously implicated in signal transduction during plant responses to pathogen attack as well as to various environmental stresses. We have isolated from rice a new MAPK cDNA, OsBIMK1 (Oryza sativa L. BTH-induced MAPK 1), which encodes a 369-amino-acid protein with moderate to high nucleotide sequence similarity to previously reported plant MAPK genes. OsBIMK1 contains all 11 of the MAPK conserved subdomains and the phosphorylation-activation motif, TEY. We analyzed in detail the expression of OsBIMK1 upon treatment with various chemicalan d biologicalinducers of resistance responses in rice and in both incompatible and compatible interactions between rice and Magnaporthe grisea. Expression of OsBIMK1 was activated rapidly upon treatment with benzothiadiazole (BTH) as well as with dichloroisonicotinic acid, probenazole, jasmonic acid and its methyl ester, Pseudomonas syringae pv. syringae, or wounding. Expression of Os-BIMK1 was induced rapidly during the first 36h after inoculation with M. grisea in BTH-treated rice seedlings and in an incompatible interaction between M. grisea and a blast-resistant rice genotype. BTH treatment induced a systemic activation ofOsBIMK1 expression. These results suggest that OsBIMK1 plays an important role in rice disease resistance.
Benzothiadiazole, Defense response, Mitogen-activated protein kinase, Oryza (, disease resistance), , BIMK1
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宋凤鸣, Fengming Song, Robert M. Goodman*
Gene 290(2002)115-124,-0001,():
-1年11月30日
Expression of the Sar8.2 gene family is induced by salicylic acid (SA) in tobacco during induction of systemic acquired resistance. Expression of Sar8.2b, one member of this 12-member family, was detected as early as 12 h after treatment with SA and was maximal 36 h after SA treatment. In NahG transgenic tobacco plants, benzothiadiazole and dichloroisonicotinic acid induced expression of Sar8.2b but SA did not, suggesting that expression of the Sar8.2b gene is SA-dependent. Several putative cis-acting elements were found in the Sar8.2b gene promoter region, including an as-1 element and GT-1 and Dof binding sequences. We constructed a series of progressive deletion mutations in the Sar8.2b promoter region linked to the b-glucuronidase (GUS) coding region and analyzed GUS activities by stable expression in transformants of Arabidopsis thaliana. Deletions between 2728 and 2927 bp or between 2351 and 2197 bp of the promoter region resulted in a significant reduction in GUS activity induced by SA treatment as shown in stable transformants of A. thaliana. The 2197 bp fragment of the promoter region was found to confer a relatively low level of GUS activity induced by SA treatment in stable expression of transformants in A. thaliana. The results suggest that 927 bp of the Sar8.2b gene promoter confers full promoter activity and that cis-acting elements required for high-level SA-inducible expression of the Sar8.2b gene may exist within the regions 2728 to 2927 bp and 2197 to 2351 bp.
Promoter, Sar8., 2b, Systemic acquired resistance, Tobacco, Nicotiana tabacum
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宋凤鸣, Fengming Song and Robert M. Goodman
MPMI Vol. 14, No.12, 2001, pp. 1458~1462.,-0001,():
-1年11月30日
When tobacco plants were treated by injection with nitric oxide (NO)-releasing compounds, the sizes of lesions caused by Tobacco mosaic virus (TMV) on the treated leaves and on upper nontreated leaves were significantly reduced. The reduction in TMV lesion size was caused by NO released from the NO-releasing compounds; the byproduct formed after release of NO from the NO-releasing compound NOC-18, diethylenetriamine, did not itself alter lesion size. Treatment of tobacco plants with inhibitors of nitric oxide synthase or an NO scavenger attenuated but did not abolish the systemic acquired resistance (SAR) induced by salicylic acid (SA). In NahG transgenic tobacco plants, NO had no effect on lesion size following TMV infection. These results are consistent with the hypothesis that NO plays an important role in SAR induction in tobacco and that NO is required for the full function of SA as an SAR inducer. The activity of NO is fully dependent on the function of SA in the SAR signaling pathway in tobacco.
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