伦照荣
长期从事寄生原虫特别是锥虫的流行病学,抗药性和分子生物学等研究
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- 姓名:伦照荣
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学术头衔:
博士生导师, 国家杰出青年科学基金获得者
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动物学
- 研究兴趣:长期从事寄生原虫特别是锥虫的流行病学,抗药性和分子生物学等研究
伦照荣,1989毕业于中山大学,获理学博士学位。瑞士热带医学研究院和多伦多大学动物学系博士后。加拿大国家自然科学和工程学研究委员会(NSERC)国际杰出青年科学家奖获得者。加拿大国家食品检验局寄生虫学研究中心研究科学家。2002,6-现在,中山大学生命科学院教授,博士生导师;动物学国家重点学科(寄生虫学)学术带头人;中山大学华南寄生生物研究中心主任;国际人畜共患病组织(OIE/NTTAT)高级科学顾问.
长期从事寄生原虫特别是锥虫的流行病学,抗药性和分子生物学等研究。在国内曾首次将伊氏锥虫在体外培养成功。在分子水平上证实伊氏锥虫与马媾疫锥虫的亲缘关系远远高于布氏锥虫。首次对分离于我国的马媾疫锥虫的动基体DNA进行研究,确证其大环和小环两种kDNA的存在并发现其大环kDNA所缺损的基因与其他马媾疫锥虫株有明显的差异,提出伊氏锥虫应直接起源于马媾疫锥虫的新观点,修订了已故著名锥虫学研究权威,英国皇家学会会员C.A.Hoare教授提出的伊氏锥虫起源于布氏锥虫,马媾疫锥虫起源于伊氏锥虫这一长达半个多世纪的假说,并提出伊氏锥虫的广泛流行与其失去大环kDNA有直接的关系。通过系统对广泛流行我国的华支睾吸虫的生物学和流行病学等进行深入的研究,提出控制华支睾吸虫病的措施,文章发表在英国著名的医学杂志《The Lancet Infectious Diseases》,是迄今为止有关华支睾吸虫的生物学和流行病学最具代表性的论文。目前主要从事寄生生物(主要是寄生原虫)与宿主细胞的分子关系、寄生生物分子流行病学和致病基因起源以及锥虫疫苗等的研究。
在国内外核心学术刊物如《科学通报》,The Lancet Infectious Diseases, Emerging Infectious Diseases, Trends in Parasitology,Molecular and Biochemical Parasitology, Molecular and Cellular Probes等发表相关文章60余篇,其中40多篇为SCI所收录。Trends in Parasitology,Journal of Eukaryotic Microbiology,Parasitolgy Research,Acta Tropica, Cell Research,《动物学报》和《动物学研究》等国内外刊物的审稿人。
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伦照荣, Feng-Jun Li a, Robin B. Gasser b, Jia-Yu Zheng a, Filip Claes c, Xing-Quan Zhu d, Zhao-Rong Lun a, *
F.-J. Li et al./Molecular and Cellular Probes xx (2005)1-8,-0001,():
-1年11月30日
Three different DNA fingerprinting techniques, the mobile genetic element (MGE)-PCR, simple sequence repeat (SSR)-PCR and random amplified polymorphic DNA (RAPD)-PCR, were used to define a large set of genetic markers to study genetic similarity within and among Trypanosoma brucei, Trypanosoma equiperdum and Trypanosoma evansi strains (nZ18) from China, Africa and South America and to investigate their genetic relationships. Using the three fingerprinting techniques, O890 bands (ranging in size from 0.2 to 2 kb) were defined for all 18 strains of Trypanosoma. Within each of the strains, 39-59 bands were defined. The similarity coefficients between strains ranged fromw41 to 94%, with a mean of 65%. There was more genetic similarity among strains within T. evansi (mean ofw79%) compared with T. equiperdum (w65%) and T. brucei (w59%). The similarity coefficient data were used to construct the dendrogram, which revealed that (irrespective of species) the majority of strains from China and South America grouped together to the exclusion of those from Africa. The exceptions were a T. brucei strain from Africa and a T. equiperdum strain of unknown origin. Hence, employing data sets generated using the three different fingerprinting methods, it was not possible to unequivocally distinguish among T. brucei, T. evansi and T. equiperdum, although there was a tendency for T. evansi strains to group together to the exclusion of T. brucei. The findings provide support for the hypothesis that T. evansi originated from a mutated form of T. equiperdum and stimulate further investigations of the genetic make-up and evolution of members of the subgenus Trypanozoon.
Trypanozoon, Mobile genetic element (, MGE), , Simple sequence repeat (, SSR), , Random amplification of polymorphic DNA (, RAPD), , Genetic variability and relationships
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【期刊论文】Clonorchiasis: a key foodborne zoonosis in China
伦照荣, Zhao-Rong Lun, Robin B Gasser, De-Hua Lai, An-Xing Li, Xing-Quan Zhu, Xing-Bing Yu, and Yue-Yi Fang
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-1年11月30日
The oriental liverfluke, Clonorchis sinensis, is of major socioeconomic importance in parts of Asia, including China, Japan, Korea, Taiwan, and Vietnam. The parasite is transmitted via snails to freshwater fish, and then to human beings and other piscivorous mammals, and causes substantial clinical or subclinical disease, known as clonorchiasis. There is considerable evidence for an aetiological relation between clonorchiasis and cholangiocarcinoma in human beings. It is estimated that about 35 million people are infected globally, of whom approximately 15 million are in China. Although very little information from China has been published in the English language, recent analyses of epidemiological data sets suggest that clonorchiasis is having an increased human-health impact due to the greater consumption of raw freshwater fish. To gain an improved insight into clonorchiasis in China, this review provides a background on the parasite and its life cycle, summarises key aspects regarding the pathogenesis, diagnosis, and treatment of clonorchiasis, describes the geographic distribution and prevalence of clonorchiasis, and makes some recommendations for future research and the control of this important disease.
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【期刊论文】Animal-to-Human SARS-associated Coronavirus Transmission?
伦照荣, Nicoletta Lari, * Laura Rindi, * Daniela Bonanni, * Enrico Tortoli, † and Carlo Garzelli*
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-1年11月30日
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伦照荣, Zhao-Rong Lun, An-Xing Li, Xiao-Guang Chen, Li-Xin Lu, Xing-Quan Zhu
,-0001,():
-1年11月30日
A total of 20 random primers (10-mers) were used to amplify RAPD markers from the genomic DNA of four Trypanosoma brucei stocks from East and West Africa, four T. evansi stocks from Africa, Asia and South America and one T. equiperdum stock from Asia. Between 65 and 88 reproducible fragments ranging from 0.25 to 2.15 kb were generated from these stocks depending on the stock/primer combination. The imilarity coefficient (SC) among the stocks of T. brucei from Kenya, Nigeria, Tanzania and Zambia ranged from 62.9% to 74.0% (average: 67.6%). The SC among the stocks of T. evansi from Kenya, China and Brazil was 76.4%-95.5% (average: 86.4%), while the SC between T. evansi stock from China and Brazil was 95.5%. For T. evansi and T. equiperdum, the SC among the stocks ranged from 81.2% to 94.4% (average: 87.6%). As for the SC among the stocks of T. brucei and T. evansi, it was found to be from 54.7% to 80.3% (average: 68.0%) and the SC among stocks of T. brucei and T. equiperdum was from 59.4% to 76.9% (average: 68.1%). Our results indicate that the stocks of T. evansi from China and from Brazil are more closely related to the stock of T. equiperdum from China than to the stocks of T. evansi isolated from Kenya and to the stocks of T. brucei. In addition, our results further support the hypothesis that T. evansi stocks from China and Brazil could have arisen from a single lineage. The possible evolution of T. evansi and T. equiperdum is also discussed.
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【期刊论文】Analysis of the CD40 CD40L role in the sustenance of alloreactive antibody production
伦照荣, Ahmed S. Shoker a, b, Zhao-Rong Lun a, Rezvan Choudry a, Anurag Saxen a
A.S. Shoker et al. Transplant Immunology 8(2001)219~228,-0001,():
-1年11月30日
CD40 ligandŽCD40L.is important for TB lymphocyte interaction. To understand the cellular basis of humoral allosensitization we, therefore: Ž1. measured CD40L protein and gene expression in sensitized and non-sensitized uremic unactivated peripheral CD4 T lymphocytes; Ž2. studied the impact of blocking the CD40CD40L pathway on alloreactive antibodyŽallo-Ab. production by engrafted sensitized PBLs into severe combined immunodeficientŽSCID.mice after in vitro preactivation with IL2LPsHLA class II allopeptides and adjuvants as a potent stimulus to produce allo-Ab ŽShoker et al. Transplantation 1999;68;1188.; andŽ3.studied the modifying effect of CD40 CD40L blockade on T helper type I and II cytokine gene expression in the respective mice spleen. The CD40L protein was measured by flow cytometry and the gene expression was measured by quantitative RT-PCR. Alloreactive antibodiesŽalo-Abs.produced by sensitized PBLs engrafted into SCID mice with and without blockade of the CD40 receptor were measured by the PRA-STAT ELISA method. The modifying effects of CD40 blocking on allo-Ab production and cytokine gene expression by the engrafted cells measured by RT-PCR were then compared. There was no detectable CD40L protein expression in either the uremic or the control groups. The CD40L gene expression of 0.04 0.02 attomolesŽaM.in the sensitized group was significantly higher than in the non-sensitized patientsŽ0.009 0.007 aM, P 0.0001. or the control CD4 T cellsŽ0.016 0.004 aM, P0.001.. Blockade of the CD40 receptor abrogated the production of allo-Ab antibodies by the engrafted sensitized cells in 60% of the tested miceŽn 10.; decreased the mean S.D. optic density of allo-Ab to 0.1 0.13 and the meanS.D. PRA to 1216.. In the presence of the control Ab, allo-Ab production in SCID sera was present in 100% of the 10 SCID mice tested; the meanS.D. PRA was 7520, and the meanS.D. OD activity was 0.4120.17. All cytokine genes were, otherwise, expressed in the presence or absence of CD40 blockade. The results suggest a potential role of an enhanced CD40 CD40L interaction in the sustenance of alloreactive antibody production without significant deviation to T helper-like I or II responses. Blocking the CD40 CD40L pathway may have a potential therapeutic benefit to treat sensitized uremic patients. 2001 Elsevier Science B.V. All rights reserved.
Alloreactive antibodies, CD40 ligand, SCID mice, Gene expression
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伦照荣, Reto Bruna*, Hermann Heckera, Zhao-Rong Lunb
R. Brun et al./Veterinary Parasitology 79(1998) 95~107,-0001,():
-1年11月30日
Trypanosoma evansi and T. equiperdum were compared regarding their ultrastructure, their mammalian hosts, way of transmission, pathogenicity, diagnosis and treatment, and biochemical and molecular characteristics. Electron microscopic investigation revealed no ultrastructural differences between the two species except that there were more coated vesicles in the flagellar pocket of T. equiperdum. Biological, biochemical and molecular studies were reviewed and exhibited many similarities between T. evansi and T. equiperdum. The most prominent differences between the two species are the presence of maxicircles in T. equiperdum, which are missing in T. evansi, and the route of transmission. While T. evansi is transmitted by biting flies, T. equiperdum is transmitted from one equine host to another during copulation when mucous membranes come into contact. Otherwise the two species are remarkably similar. The phylogenetic relationship between the two species and T. b. brucei is being discussed, and the hypothesis is proposed that T. evansi arose from a clone of T. equiperdum which lost its maxicircles. # 1998 Elsevier Science B.V. All rights reserved.
Trypanosoma evansi, Trypanosoma equiperdum, Ultrastructure, Phylogenetic relationship
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伦照荣, &roles:Z.-R. Lun
Parasitol Res (1996)82: 22-27,-0001,():
-1年11月30日
A total of 20 decamer primers were used to generate random applied polymorphic DNA (RAPD) markers from 5 isolates of Trypanosoma fallisi, 3 isolates of T. ranarum, 2 isolates of T. rotatorium, and 2 isolates of T. rotatorium-like trypanosomes in addition to 2 species from the American Type Culture Collection, T. chattoni (ATCC 50294) and Trypanosoma sp. (ATCC 50295). A slight polymorphism was observed among the four isolates of T. fallisi obtained from American toads, Bufo americanus, collected in Algonquin Park, Ontario, Canada, and an isolate obtained from the same species of host collected in Marquette, Michigan, United States, and produced similarity coefficients ranging from 80.7% to 96.9%. Pronounced polymorphism was recorded among the three isolates of T. ranarum from bullfrogs, Rana catesbeiana, collected in Ontario, Canada, and in Maryland, United States, and from a Northern leopard frog, R. pipiens, collected in Minnesota (USA). The similarity coefficients ranged from 54.7% to 59.5%, suggesting that alleles of these isolates were conserved over a wide geographic range. The high degree of polymorphism observed in two isolates of T. rotatorium from a bullfrog collected in Ontario and two isolates of a T. rotatorium-like parasite from the green frog R. clamitans, collected in Louisiana (USA) suggests that they are different species. These results reflect the high similarity among isolates from the same geographic location and the pronounced polymorphism apparent among isolates from distant geographic locations.
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【期刊论文】Stimulative effects of insulin on Toxoplasma gondii replication in 3T3-L1 cells
伦照荣, Sha Zhu, Zhao-Rong Lun*, De-Hua Lai, San-Qiang Li
S. Zhu et al./Cell Biology International xx (2005)1-5,-0001,():
-1年11月30日
The influence of insulin and 2-deoxy-glucose (D-glucose) on the intracellular protozoan Toxoplasma gondii replication in 3T3-L1 cells was investigated. Insulin and D-glucose had a dose-responsive mitogenic effect on intracellular T. gondii replication and development in 3T3-L1 cells. Insulin concentrations between 102 and 101 mg/ml combination of 4.5g/l D-glucose in DMEM medium gave maximum stimulus to T. gondii replication. The number of tachyzoites increased rapidly, with the growth peaking typically on day 3 or 4 of culture, and then declining quickly. However, insulin, in the absence of D-glucose, had comparably less effect on T. gondii growth than two of their combination. D-glucose concentrations significantly affected the tachyzoite replication and appear to be indispensable for maintaining the host 3T3-L1 cells. 2005 Published by Elsevier Ltd on behalf of International Federation for Cell Biology.
Insulin, D-glucose, Toxoplasma gondii, 3T3-L1 cells
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【期刊论文】Are Tritrichomonas foetus and Tritrichomonas suis synonyms?
伦照荣, Zhao-Rong Lun , Xiao-Guang Chen , Xing-Quan Zhu , Xiang-Rui Li and Ming-Quan Xie
TRENDS in Parasitology Vol.21 No.3 March 2005,-0001,():
-1年11月30日
Tritrichomonas suis, a tritrichomonad of pigs, and the related species Tritrichomonas foetus, a tritrichomonad of cattle, are morphologically identical. The taxonomic relationship between these two tritrichomonads has been questioned ever since they were established as distinct species in 1843 and 1928, respectively. Here, we compare the similarities of morphology, ultrastructure, distribution, host specificity, characteristics of in vitro cultivation, immunology, biochemistry and analysis of molecular data from published sources between these two species. All data indicate that these two tritrichomonad species are identical. Thus, we propose that T. foetus and T. suis are synonyms.
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