您当前所在位置: 首页 > 学者
为您找到 19 条相关结果
按相关度
  • 按相关度
  • 按时间
  • 按阅读量
  • 代表性成果优先

上传时间

2012年03月24日

【期刊论文】Genetic and molecular analysis of the role played by DELLA proteins in the control of trichome development in Arabidopsis.

甘银波, Gan, YB., Yu, H., Peng J and Broun., P.

Plant Physiology,-0001,():

摘要

暂无

关键词: GIS,, GA,, DELLA

  • 8浏览

  • 0点赞

  • 0收藏

  • 0分享

  • 61下载

  • 0评论

  • 引用

上传时间

2015年08月09日

  • 71浏览

  • 0点赞

  • 0收藏

  • 0分享

  • 4下载

  • 0评论

  • 引用

上传时间

2015年08月09日

  • 27浏览

  • 0点赞

  • 0收藏

  • 0分享

  • 3下载

  • 0评论

  • 引用

上传时间

2015年08月09日

【期刊论文】Dynamic localization and the associated translocation mechanism of HMGBs in response to GCRV challenge in CIK cells

苏建国, Youliang Rao, Jianguo Su*, Chunrong Yang, Nana Yan, Xiaohui Chen and Xiaoli Feng

Cellular & Molecular Immunology 2015; 12(3): 342-353.,-0001,():

摘要

暂无

关键词: grass carp (, Ctenopharyngodon idella), grass carp reovirus, HMGBs, innate immunity, subcellular localization

  • 28浏览

  • 0点赞

  • 0收藏

  • 0分享

  • 3下载

  • 0评论

  • 引用

上传时间

2014年04月01日

【期刊论文】Identification, characterization and immunological response analysis of stimulator of interferon gene (STING) from grass carp Ctenopharyngodon idella

苏建国, Xiaoli Feng, Chunrong Yang, Yixuan Zhang, Limin Peng, Xiaohui Chen, Youliang Rao, Tianle Gu, Jianguo Su*

Developmental & comparative immunology,-0001,():

摘要

暂无

关键词: Grass carp (, Ctenopharyngodon idella), STING, PAMPs, Grass carp reovirus, Immune responses

  • 75浏览

  • 0点赞

  • 0收藏

  • 0分享

  • 16下载

  • 0评论

  • 引用

上传时间

2014年04月01日

  • 31浏览

  • 0点赞

  • 0收藏

  • 0分享

  • 17下载

  • 0评论

  • 引用

上传时间

2014年04月01日

  • 18浏览

  • 0点赞

  • 0收藏

  • 0分享

  • 2下载

  • 0评论

  • 引用

上传时间

2014年04月01日

  • 32浏览

  • 0点赞

  • 0收藏

  • 0分享

  • 1下载

  • 0评论

  • 引用

上传时间

2014年04月01日

【期刊论文】Characterizations of two grass carp Ctenopharyngodon idella HMGB2 genes and potential roles in innate immunity

苏建国, Youliang Rao, Jianguo Su*, Chunrong Yang, Limin Peng, Xiaoli Feng, Qingmei Li

Developmental & comparative immunology,-0001,():

摘要

暂无

关键词: Grass carp (, Ctenopharyngodon idella), HMGB2, Grass carp reovirus, Innate immunity, Antiviral activity

  • 51浏览

  • 0点赞

  • 0收藏

  • 1分享

  • 2下载

  • 0评论

  • 引用

上传时间

2014年04月01日

  • 35浏览

  • 0点赞

  • 0收藏

  • 0分享

  • 1下载

  • 0评论

  • 引用

上传时间

2014年04月01日

  • 28浏览

  • 0点赞

  • 0收藏

  • 0分享

  • 1下载

  • 0评论

  • 引用

上传时间

2014年04月01日

  • 782浏览

  • 0点赞

  • 0收藏

  • 1分享

  • 15下载

  • 0评论

  • 引用

上传时间

2011年01月04日

  • 26浏览

  • 0点赞

  • 0收藏

  • 0分享

  • 149下载

  • 0评论

  • 引用

上传时间

2011年01月04日

  • 42浏览

  • 0点赞

  • 0收藏

  • 0分享

  • 109下载

  • 0评论

  • 引用

上传时间

2011年03月27日

  • 20浏览

  • 0点赞

  • 0收藏

  • 0分享

  • 134下载

  • 0评论

  • 引用

上传时间

2011年03月27日

  • 37浏览

  • 0点赞

  • 0收藏

  • 0分享

  • 280下载

  • 0评论

  • 引用

上传时间

2010年08月30日

【期刊论文】Molecular cloning and immune responsive expression of MDA5 gene, a pivotal member of the RLR gene family from grass carp Ctenopharyngodon idella

苏建国, Jianguo Su*, Teng Huang, Jie Dong, Jianfu Heng, Rongfang Zhang, Limin Peng

Fish & Shellfish Immunology 28(2010)712-718,-0001,():

摘要

The cytoplasmic helicase protein MDA5 (melanoma-differentiation-associated gene-5) recognizes long molecules of viral double-stranded RNA (dsRNA) and single-stranded RNA with 50 triphosphate and mediates type I interferon secretion. In the present study, the first MDA5 gene in fish was cloned and characterized from grass carp Ctenopharyngodon idella. The full length of the C. idella MDA5 (CiMDA5) cDNA is 3233 nucleotides in length and encodes a polypeptide of 961 amino acids. The deduced amino acid sequence contained six main structural domains: a CARD (caspase activation and recruitment domain), a DEXDc (DEAD/DEAH box helicase domain), a ResIII (conserved restriction domain of bacterial type III restriction enzyme), two HELICc (helicase superfamily c-terminal domain) and an RD (regulatory domain). The CiMDA5 mRNAwas widespread expression in the tested tissues, was high level in spleen, skin and gill tissues, and was up-regulated by GCRV injection by semi-quantitative RT-PCR assay. The CiMDA5 transcripts in spleen were significantly up-regulated at 12 h (1.80 folds, P< 0.05), reached the crest at 24 h (7.48 folds, P<0.05), and then recovered to normal level at 48 h post-injection (P>0.05) of grass carp reovirus (GCRV). In liver, the temporal expression of CiMDA5 mRNA was significantly increased at 48 h (5.00 folds, P<0.05) and returned to control level at 72 h (P>0.05) after GCRV challenge. These data implied that CiMDA5 involved in the early stage of antiviral innate immune defense to GCRV in grass carp, and provided new insight into the evolution research of RLR (RIG-I like receptor) gene family.

关键词: Grass carp (, Ctenopharyngodon idella), ,, MDA5,, RLR,, Gene cloning,, mRNA expression,, ., Grass carp reovirus

  • 75浏览

  • 0点赞

  • 0收藏

  • 0分享

  • 159下载

  • 0评论

  • 引用

上传时间

2010年08月30日

【期刊论文】Identification andexpressionprofilinganalysisofgrasscarp CtenopharyngodonidellaLGP2 cDNA

苏建国, TengHuang, JianguoSu*, JianfuHeng, JieDong, RongfangZhang, HuimingZhu

Fish &Shellfish Immunology 29(2010)349-355,-0001,():

摘要

LGP2 (laboratoryofgeneticsandphysiology2), ahomologueof RIG-I (Retinoicacidinduciblegene-I)and MDA5 (Melanomadifferentiationassociatedgene5)withouttheCARD (caspaseactivationandrecruitment domain)requiredforsignaling, playsapivotalroleinmodulatingsignalingby RIG-I andMDA5 for interferon (IFN)synthesis. Inthisstudy, anovel LGP2 genefromgrasscarp Ctenopharyngodonidella (designatedas CiLGP2) wasisolatedandcharacterized. Thefull-lengthcDNAof CiLGP2 wasof2920bpwith fiveinstability motifs (ATTTA). Theopenreadingframewasof2043bpandencodedapolypeptideof680aminoacids, including fivemainoverlappingstructuraldomains:twoDEXDc (DEAD/DEAHboxhelicasedomain), one ResIII (conservedrestrictiondomainofbacterialtypeIIIrestrictionenzyme), oneHELICc (helicase superfamilyc-terminaldomain)andoneRD (regulatorydomain). Therewasonemore a-helixintheRD, comparedwiththatinhuman. The CiLGP2 mRNAwasubiquitousexpressioninthetestedtissues, washigh levelinspleen, skin, heartandintestinetissues, andwasup-regulatedbygrasscarpreovirus (GCRV) injectionbysemi-quantitativeRT-PCR (sqRT-PCR)assay. The CiLGP2 expressioninspleenwassignificantly up-regulatedat12h (14.5 folds, P<0.05), reachedthecrestat24h (19.0 folds, P<0.05), andthendropped a littleat48h (10. 4folds)post-injectionofGCRVandkeptthislevelinthefollowingtestperiod (P<0.05). In liver, thetemporalexpressionof CiLGP2 mRNAwassignificantlyincreasedat24h (3.8 times, P<0.05), reachedpeakat 48h (10.7 times, P<0.05), andthendecreasedalittlebitat72h (5.8times, P<0.05) and keptthishighlevelbytheendofthetest (P<0.05). Theseresultscollectivelysuggestedthat CiLGP2 was a novelmemberofRLRgenefamily, engagingintheearlystageofantiviralinnateimmunedefenseingrass carp, andlaidthefoundationforthefurthermechanismresearchof LGP2 in fishes.

关键词: Grass carp (, Ctenopharyngodon idella), LGP2 Gene cloning mRNAexpression Grass carpreovirus

  • 25浏览

  • 0点赞

  • 0收藏

  • 1分享

  • 114下载

  • 0评论

  • 引用

上传时间

2010年08月30日

【期刊论文】Genomic organization and expression analysis of Toll-like receptor 3 in grass carp (Ctenopharyngodon idella)

苏建国, Jianguo Su a, *, Songhun Jang b, Chunrong Yang a, Yaping Wang b, Zuoyan Zhu b

Fish & Shellfish Immunology 27(2009)433-439,-0001,():

摘要

investigate grass carp immune system responding to GCRV (grass carp reovirus) infection, the full-length cDNA sequence and genomic organization of grass carp TLR3 (CiTLR3) was identified and characterized. The full-length genome sequence of CiTLR3 is composed of 5668 nucleotides, including five exons and four introns. The full-length of CiTLR3 cDNA is 3681 bp in length and encodes a polypeptide of 904 amino acids with an estimated molecular mass of 102,765 Da and a predicted isoelectric point of 8.35. Analysis of the deduced amino acid sequence indicated that CiTLR3 has four main structural domains, including a signal peptide sequence, 14 LRR (leucine-rich repeat) motifs, a transmembrane region and a TIR (Toll/interleukin-1 receptor) domain. It is most similar to the crucian carp (Carassius auratus) TLR3 amino acid sequence with an identity of 99%. Quantitative RT-PCR analysis showed that CiTLR3 transcripts were significantly up-regulated starting at day 1 and continued through day 7 following GCRV infection (P<0.05). These data implied that CiTLR3 is involved in antiviral defense, provide molecular and functional information for grass carp TLR3, and implicate their role in mediating immune protection against grass carp viral diseases.

关键词: Ctenopharyngodon idella,, Toll-like receptor 3,, Genomic organization,, Gene cloning,, Grass carp reovirus

  • 24浏览

  • 0点赞

  • 0收藏

  • 0分享

  • 94下载

  • 0评论

  • 引用