- 水产养殖学 18
- 0000年 18
- 期刊论文 18
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- Developmental & comparative immunology 4
- Fish & Shellfish Immunology 2
- Cellular & Molecular Immunology 2015; 12(3): 342-353. 1
- Developmental & Comparative Immunology 2015; 48(1):86-94. 1
- Developmental & comparative immunology 1
- Fish & Shellfish Immunology 27(2009)433-439 1
- Fish & Shellfish Immunology 28(2010)712-718 1
- Fish & Shellfish Immunology 30 (2011) 830-835 1
- Fish & Shellfish Immunology 30 (2011) 936-943 1
- Journal of Fish Biology (2011) 78, 973-979 1
- PLoS One 1
- Scientific Reports 2015; 5: 12946. 1
- 苏建国 18
- Jianguo Su* 13
- Chunrong Yang 7
- Limin Peng 6
- Chunrong Yang 5
- Rongfang Zhang 5
- Teng Huang 4
- Jie Dong 3
- Qingmei Li 3
- Xiaohui Chen 3
- Jianguo Su* 3
- Youliang Rao 3
- 华中农业大学,湖北 18
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苏建国, Quanyuan Wan, Jianguo Su*
Scientific Reports 2015; 5: 12946.,-0001,():
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关键词: Grass carp (, Ctenopharyngodon idella), , RNA-seq, MiSeq, Alternative splice, Grass carp reovirus
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苏建国, Xueying Shang, Jianguo Su*, Quanyuan Wan, Juanjuan Su
Developmental & Comparative Immunology 2015; 48(1):86-94.,-0001,():
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关键词: Grass carp (, Ctenopharyngodon idella), , MDA5, CpA, DNA methylation, mRNA expression, Grass carp reovirus
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26浏览
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苏建国, Youliang Rao, Jianguo Su*, Chunrong Yang, Nana Yan, Xiaohui Chen and Xiaoli Feng
Cellular & Molecular Immunology 2015; 12(3): 342-353.,-0001,():
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关键词: grass carp (, Ctenopharyngodon idella), , grass carp reovirus, HMGBs, innate immunity, subcellular localization
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28浏览
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苏建国, Xiaoli Feng, Chunrong Yang, Yixuan Zhang, Limin Peng, Xiaohui Chen, Youliang Rao, Tianle Gu, Jianguo Su*
Developmental & comparative immunology,-0001,():
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关键词: Grass carp (, Ctenopharyngodon idella), , STING, PAMPs, Grass carp reovirus, Immune responses
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73浏览
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苏建国, Chunrong Yang, Qingmei Li, Jianguo Su*, Xiaohui Chen, Yaping Wang, Limin Peng
Developmental & comparative immunology,-0001,():
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关键词: Grass carp (, Ctenopharyngodon idella), , TRIF, Genomic structure, promoter activity, antiviral immunity, Grass carp reovirus
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31浏览
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苏建国, Chunrong Yang, Lijun Chen, Jianguo Su*, Xiaoli Feng, Youliang Rao
Fish & Shellfish Immunology,-0001,():
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关键词: Grass carp (, Ctenopharyngodon idella), , HMGB3, Grass carp reovirus, Innate immunity, Signal transduction
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18浏览
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【期刊论文】Protective roles of grass carp Ctenopharyngodon idella Mx isoforms against grass carp reovirus
苏建国, Limin Peng, Chunrong Yang, Jianguo Su*
PLoS One,-0001,():
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关键词: Grass carp (, Ctenopharyngodon idella), , Mx, Antiviral activity, Grass carp reovirus, Type I interferon
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32浏览
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苏建国, Youliang Rao, Jianguo Su*, Chunrong Yang, Limin Peng, Xiaoli Feng, Qingmei Li
Developmental & comparative immunology,-0001,():
暂无
关键词: Grass carp (, Ctenopharyngodon idella), , HMGB2, Grass carp reovirus, Innate immunity, Antiviral activity
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50浏览
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苏建国, Chunrong Yang, Limin Peng, Jianguo Su*
Developmental & comparative immunology,-0001,():
暂无
关键词: Grass carp (, Ctenopharyngodon idella), , HMGB1, PAMPs, mRNA expression, Cell proliferation, Grass carp reovirus
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35浏览
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苏建国, Quanyuan Wan, Jianguo Su*, Xiaohui Chen, Chunrong Yang
Developmental & comparative immunology,-0001,():
暂无
关键词: Grass carp (, Ctenopharyngodon idella), , IPS-1, Genetic polymorphism, Haplotype, Genomic organization, Grass carp reovirus
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28浏览
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苏建国, Chunrong Yang, Jianguo Su*, Qingmei Li, Rongfang Zhang, Youliang Rao
Fish & Shellfish Immunology,-0001,():
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关键词: Grass carp (, Ctenopharyngodon idella), , Antiviral immunity, ADAR1, mRNA expression, Grass carp reovirus------WebKitFormBoundaryGeovirus
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781浏览
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苏建国, Jianfu Heng, Jianguo Su*, Teng Huang, Jie Dong, Lijun Chen
Fish & Shellfish Immunology 30 (2011) 45-50,-0001,():
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关键词: Grass carp (, Ctenopharyngodon idella), ,, TLR3,, Polymorphism,, Haplotype,, Grass carp reovirus
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26浏览
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苏建国, Jianguo Su*, Teng Huang, Chunrong Yang, Rongfang Zhang
Fish & Shellfish Immunology 30 (2011) 317-323,-0001,():
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关键词: Grass carp (, Ctenopharyngodon idella), ,, IPS-1,, Gene cloning,, mRNA expression,, Grass carp reovirus
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35浏览
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苏建国, Chunrong Yang, Jianguo Su*, Teng Huang, Rongfang Zhang, Limin Peng
Fish & Shellfish Immunology 30 (2011) 936-943,-0001,():
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关键词: Grass carp (, Ctenopharyngodon idella), , RIG-I, Gene cloning, mRNA expression, Grass carp reovirus
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20浏览
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苏建国, J. Su*, J. Dong, T. Huang, R. Zhang, C. Yang and J. Heng
Journal of Fish Biology (2011) 78, 973-979,-0001,():
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35浏览
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苏建国, Jianguo Su*, Rongfang Zhang, Jie Dong, Chunrong Yang
Fish & Shellfish Immunology 30 (2011) 830-835,-0001,():
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关键词: Ctenopharyngodon idella, Internal control genes, Tissues, Cell culture, Grass carp reovirus
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37浏览
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苏建国, Jianguo Su*, Teng Huang, Jie Dong, Jianfu Heng, Rongfang Zhang, Limin Peng
Fish & Shellfish Immunology 28(2010)712-718,-0001,():
The cytoplasmic helicase protein MDA5 (melanoma-differentiation-associated gene-5) recognizes long molecules of viral double-stranded RNA (dsRNA) and single-stranded RNA with 50 triphosphate and mediates type I interferon secretion. In the present study, the first MDA5 gene in fish was cloned and characterized from grass carp Ctenopharyngodon idella. The full length of the C. idella MDA5 (CiMDA5) cDNA is 3233 nucleotides in length and encodes a polypeptide of 961 amino acids. The deduced amino acid sequence contained six main structural domains: a CARD (caspase activation and recruitment domain), a DEXDc (DEAD/DEAH box helicase domain), a ResIII (conserved restriction domain of bacterial type III restriction enzyme), two HELICc (helicase superfamily c-terminal domain) and an RD (regulatory domain). The CiMDA5 mRNAwas widespread expression in the tested tissues, was high level in spleen, skin and gill tissues, and was up-regulated by GCRV injection by semi-quantitative RT-PCR assay. The CiMDA5 transcripts in spleen were significantly up-regulated at 12 h (1.80 folds, P< 0.05), reached the crest at 24 h (7.48 folds, P<0.05), and then recovered to normal level at 48 h post-injection (P>0.05) of grass carp reovirus (GCRV). In liver, the temporal expression of CiMDA5 mRNA was significantly increased at 48 h (5.00 folds, P<0.05) and returned to control level at 72 h (P>0.05) after GCRV challenge. These data implied that CiMDA5 involved in the early stage of antiviral innate immune defense to GCRV in grass carp, and provided new insight into the evolution research of RLR (RIG-I like receptor) gene family.
关键词: Grass carp (, Ctenopharyngodon idella), ,, MDA5,, RLR,, Gene cloning,, mRNA expression,, ., Grass carp reovirus
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苏建国, Jianguo Su a, *, , Songhun Jang b, Chunrong Yang a, Yaping Wang b, Zuoyan Zhu b
Fish & Shellfish Immunology 27(2009)433-439,-0001,():
investigate grass carp immune system responding to GCRV (grass carp reovirus) infection, the full-length cDNA sequence and genomic organization of grass carp TLR3 (CiTLR3) was identified and characterized. The full-length genome sequence of CiTLR3 is composed of 5668 nucleotides, including five exons and four introns. The full-length of CiTLR3 cDNA is 3681 bp in length and encodes a polypeptide of 904 amino acids with an estimated molecular mass of 102,765 Da and a predicted isoelectric point of 8.35. Analysis of the deduced amino acid sequence indicated that CiTLR3 has four main structural domains, including a signal peptide sequence, 14 LRR (leucine-rich repeat) motifs, a transmembrane region and a TIR (Toll/interleukin-1 receptor) domain. It is most similar to the crucian carp (Carassius auratus) TLR3 amino acid sequence with an identity of 99%. Quantitative RT-PCR analysis showed that CiTLR3 transcripts were significantly up-regulated starting at day 1 and continued through day 7 following GCRV infection (P<0.05). These data implied that CiTLR3 is involved in antiviral defense, provide molecular and functional information for grass carp TLR3, and implicate their role in mediating immune protection against grass carp viral diseases.
关键词: Ctenopharyngodon idella,, Toll-like receptor 3,, Genomic organization,, Gene cloning,, Grass carp reovirus
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