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2017年03月10日

【期刊论文】核磁共振氢谱技术评估大肠杆菌对电解水的代谢反应

杨宏顺, Liu Q, Wu J, Lim ZY, Aggarwal A, Yang H*, Wang S.

LWT - Food Science and Technology,2017,79(-):428-436

2017年01月24日

摘要

Electrolysed water (EW) is an activated liquid with a high oxidation-reduction potential. EW causes oxidative damage to pathogenic microorganisms and as a result, may have utility in the food industry. The molecular mechanism of EW's action is not understood. In this study, we exposed Escherichia coli ATCC 25922 to a sub-lethal concentration of EW and examined structural and metabolic changes. Atomic force microscopy revealed that EW caused damage to E. coli membranes. To understand the metabolic responses to EW perturbations in of E. coli, multivariate data analysis of NMR spectroscopy demonstrated that EW significantly influenced the metabolic state. This included reducing nucleotide and amino acid biosynthesis, suppressing energy-associated metabolism, altering osmotic adjustment, and promoting fatty acid metabolism. The results enrich our understanding of E. coli metabolic changes caused by EW perturbation and support the effectiveness of the NMR metabolomics as a valuable tool to analyse and evaluate such a complex biological system.

关键词: 电解水, 大肠杆菌, 氧化应激, 核磁共振, 原子力显微镜;Electrolysed water, Escherichia coli, Oxidative stress, NMR, Atomic force microscopy

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2016年12月21日

【期刊论文】鱼胶结合和壳聚糖可以有效抑制金昌鱼片在冷藏过程中肌原纤维的降解。

杨宏顺, Xiao Feng, Nidhi Bansal, Hongshun Yang

Food Chemistry,2016,200(-):283-292

2016年01月11日

摘要

Coating of gelatin and chitosan can improve fish fillet’s quality, but the mechanism is not clear. Chitosan/gelatin coatings significantly prevented deterioration of golden pomfret fillet at 4 C. Chitosan with 7.2% gelatin group showed the best effect on preserving the length of myofibril, which remained greater than 15 lm at day 17 of storage, while for control, chitosan and chitosan combined with 3.6% gelatin group, it was 5.03, 10.04 and 9.02 lm, respectively. The MALDI-TOF MS result revealed that the coatings slowed down the protein deterioration of fillet. On days 13 and 17, the myosin light chain and myoglobin in control group degraded, while the two proteins still existed in chitosan/gelatin coated groups. Overall,the chitosan with 7.2% gelatin coating had the best effect on preserving fillet’s quality during storage.The coating may exert its protective effect via inhibiting myofibril degradation within fillet.

关键词: 可食包衣, 明胶, 壳聚糖, 基质辅助激光解析电离, 飞行时间质谱, 原子力显微镜, 肌原纤维, 肌球蛋白轻链, 肌红蛋白;Edible coating, Gelatin, Chitosan, Matrix assisted laser desorption ionisation, time of flight mass spectrometry (MALDITOF-MS, Atomic force microscopy (AFM), Myofibril, Myosin light chain, Myoglobin

  • 18浏览

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2016年12月09日

【期刊论文】添加明胶提高煮鱼圆中营养保持、质地和传质但不影响纳米结构

杨宏顺, Xiao Feng, Caili Fu, Hongshun Yang

LWT-Food Science and Technology,-0001,():

摘要

暂无

关键词: 模型, 明胶, 纤维蛋白, 鱼圆, 原子力显微镜

  • 38浏览

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2017年05月02日

【期刊论文】一种能够产生中性电解水的便携式电解消毒设备的开发

杨宏顺, Jufang Zhang , Shigang Zhou , Ronghua Chen , Hongshun Yang

LWT - Food Science and Technology,2017,82(1):207-215

2017年04月10日

摘要

The aim of this study was to develop and evaluate the characteristics and performance of a portable electrolytic sanitising unit. Free available chlorine (FAC), oxidation-reduction potential, and pH of electrolysed water were measured. Response surface methodology coupled with a Box-Behnken design was used to describe the input-output relationship and optimise FAC production. A partial catholyte solution was reintroduced to electrolysis for generating neutral electrolysed water. The result found that RuO2- IrO2/TiO2 electrode was very effective. A FAC concentration of 4 mg/L achieved >2 log CFU/mL reduction, while a FAC concentration of 40 mg/L achieved >6 log CFU/mL reduction in Escherichia coli O157:H7 and Listeria monocytogenes BAA-839. The developed sanitiser had a pH of 7.08 ± 0.08, and the commercial sanitiser had a pH of 3.77 ± 0.18. The developed sanitiser had similar bactericidal effects as the commercial sanitiser. The results revealed that the developed sanitising unit is promising for the control of foodborne pathogens.

关键词: 便携式消毒设备, 响应面优化设计, 杀菌活性, 大肠杆菌O157:H7型, 单核细胞增多性李斯特氏菌;Portable sanitising unit , Box-Behnken design, Bactericidal activity, Escherichia coli O157:H7, Listeria monocytogenes

  • 136浏览

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2017年03月10日

  • 58浏览

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  • 2分享

  • 21下载

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2017年03月10日

【期刊论文】香芹酚纳米乳剂结合酸性电解水灭活鲜切卷心菜表面细菌,酵母和原生菌群

杨宏顺, Sow LC, Tirtawinata F, Yang H*, Shao Q, Wang S

Food Control,2017,76(-):88-95

2017年08月29日

摘要

Carvacrol is an effective antimicrobial agent originated from essential oils, this natural antimicrobial agent has higher consumer acceptance compared to chemical agents. Due to the low solubility of carvacrol in water, carvacrol was delivered as a nanoemulsion. A carvacrol nanoemulsion contained 3.5% (w/w) oil phase (1% carvacrol and 2.5% corn oil, w/w) and 3.5% (w/w) Tween 80 was produced by ultrasonification at 10 min using 100% amplitude; the median particle size was 309 ± 19 nm. The nanoemulsion was shelf-life stable for 1 month without any significant changes in particle size. When applied against Escherichia coli ATCC 25922 and Pichia pastoris GS115 growth in nutrient broth, carvacrol nanoemulsion (0.5% w/w carvacrol) achieved 3 log reductions of microorganisms. When microorganisms were fixed and dried on stainless steel coupon surface, the carvacrol nanoemulsion treatment was more effective on E. coli than P. pastoris with about 5 and 0.3 log reduction of viable count, respectively. The native microflora on shredded cabbages was challenged by combining carvacrol nanoemulsion and acidic electrolysed water (AEW) that contained 4 mg/L free available chlorine (FAC). The treatment reduced about 0.5 log of aerobic mesophilic and psychrotropic bacteria counts and the antimicrobial activity of carvacrol nanoemulsion and AEW lasted up to 2 days. The results indicated that carvacrol nanoemulsion is promising in controlling the safety of fresh-cut vegetables.

关键词: 纳米乳剂, 香芹酚, 纳米技术, 抗菌剂, 鲜切, 食品安全, 有机食品, 消毒, 大肠杆菌, 毕赤氏酵母, 超声强化, 高压匀浆;Nanoemulsion, Carvacrol, Nanotechnology, Antimicrobial, Fresh cut, Food safety, Organic food, Organic vegetables, Sanitisation, Escherichia coli, Pichia pastoris, Ultrasonification, High pressure homogenisation

  • 34浏览

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2014年12月19日

【期刊论文】Comprehensive study of the role of ethylene glycol when preparing Ag@SBA-15 in supercritical CO2

银建中, Qin-Qin Xu, Yu-Ling Ma, Gang-Xu, Jian-Zhong Yin*, Ai-Qin Wang, Jin-Ji Gao

The Journal of Supercritical Fluids,-0001,():

摘要

暂无

关键词: supercritical CO2, Ag nanowires , ethylene glycol, non-equilibrium sorption

  • 19浏览

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2014年12月19日

【期刊论文】INVESTIGATION OF THE EFFECT OF SUPERCRITICAL CARBON DIOXIDE PRETREATMENT ON REDUCING SUGAR YIELD OF LIGNOCELLULOSE HYDROLYSIS

银建中, YI-FAN LIU, PENG LUO, QIN-QIN XU, EN-JUN WANG and JIAN-ZHONG YIN*

CELLULOSE CHEMISTRY AND TECHNOLOGY ,-0001,():

摘要

暂无

关键词: lignocellulose,, supercritical carbon dioxide,, pretreatment,, reducing sugars

  • 31浏览

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2014年12月19日

【期刊论文】Preparation of Superhighly Dispersed Co3O4@SBA-15 with Different Morphologies in Supercritical CO2 with the Assistance of Dilute Acids

银建中, Qin-Qin Xu, Gang-Xu, Jian-Zhong Yin*, Ai-Qin Wang , Yu-Ling Ma and Jin-Ji Gao

Industrial & Engineering Chemistry Research,-0001,():

摘要

暂无

关键词: supercritical carbon dioxide, mesoporous silica, dilute acid, highly dispersed, Co3O4

  • 18浏览

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2014年12月19日

【期刊论文】Enzymatic hydrolysis enhancement of corn lignocellulose by supercritical CO2 combined with ultrasound pretreatment

银建中, Jianzhong Yin*, Liudan Hao, Wen Yu, Enjun Wang, Mengjiao Zhao, Qinqin Xu, Yifan Liu

Chinese Journal of Catalysis,-0001,():

摘要

暂无

关键词: Lignocellulose Pretreatment, Supercritical carbon dioxide, Ultrasound, Enzymatic hydrolysis

  • 17浏览

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2012年04月09日

【期刊论文】Ophiopogonin D prevents H2O2-induced injury in primary human umbilical vein endothelial cells

刘畅, Qian, J., Jiang, F., Wang, B., Yu, Y., Zhang, X., Yin, Z., & Liu, C.

J. Ethnopharmacol. (2010), 128: 438-445.,-0001,():

摘要

暂无

关键词: Endothelial injury model,, Ophiopogonin D,, Oxidative stress,, Inflammation,, Apoptosis

  • 74浏览

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  • 57下载

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2012年04月09日

【期刊论文】Protective effects of chlorogenic acid on acute hepatotoxicity induced by lipopolysaccharide in mice

刘畅, Xu, Y., Chen, J., Yu, X., Tao, W., Jiang, F., Yin, Z., & Liu, C.

Inflamm. Res. (2010), 59: 871-877.,-0001,():

摘要

暂无

关键词: Chlorogenic acid,, Liver injury,, LPS,, Inflammation

  • 26浏览

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  • 46下载

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2012年04月09日

【期刊论文】Ligustrazine improves atherosclerosis in rat via attenuation of oxidative stress

刘畅, Jiang, F., Qian, J., Chen, S., Zhang, W., & Liu, C.

Pharmaceutical Biology (2011), 49: 856-863.,-0001,():

摘要

暂无

关键词: Ligustrazine,, atherosclerosis,, oxidative stress,, dyslipidemia

  • 40浏览

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  • 49下载

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2012年04月09日

  • 17浏览

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2012年04月09日

【期刊论文】SWItch/sucrose nonfermentable (SWI/SNF) complex subunit BAF60a integrates hepatic circadian clock and energy metabolism

刘畅, Tao, W., Chen, S., Shi, G., Guo, J., Xu, Y., & Liu, C.

Hepatology (2011), 54: 1410-1420.,-0001,():

摘要

暂无

关键词: circadian clock,, metabolism,, BAF60a,, chromatin remodeling,, liver

  • 146浏览

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  • 85下载

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2014年10月18日

【期刊论文】Comparison of methods for detoxification of spruce hydrolysate for bacterial cellulose production

洪枫, Xiang Guo(郭香), Adnan Cavka, Leif J Jönsson and Feng Hong(洪枫)*

Microbial Cell Factories, 2013, 12: 93.,-0001,():

摘要

暂无

关键词: Bacterial cellulose,, Gluconacetobacter xylinus,, Norway spruce hydrolysate,, Detoxification methods

  • 45浏览

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  • 23下载

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2014年10月18日

【期刊论文】Production of bacterial cellulose and enzyme from waste fiber sludge

洪枫, Adnan Cavka, Xiang Guo(郭香), Shui-Jia Tang(唐水佳), Sandra Winestrand, Leif J. Jönsson, Feng Hong(洪枫)*

Biotechnology for Biofuels, 2013; 6: 25.,-0001,():

摘要

暂无

关键词: Bacterial cellulose,, Gluconacetobacter xylinus,, Enzyme production,, Cellulase,, Trichoderma reesei,, Fiber sludge

  • 13浏览

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  • 14下载

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2013年04月17日

【期刊论文】Biotransformation of wheat straw to bacterial cellulose and its mechanism

洪枫, Lin Chen(陈琳), Feng Hong(洪枫)*, Xue-xia Yang(杨雪霞), Shi-fen Han(韩士芬)

Bioresource Technology, 2013, 135, 464-468,-0001,():

摘要

暂无

关键词: Bacterial cellulose,, Biorefinery,, Wheat straw,, Ionic liquid pretreatment,, 1-Allyl-3-methylimidazolium chloride (, [AMIM]Cl),

  • 24浏览

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