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杨宏顺, Liu Q, Wu J, Lim ZY, Aggarwal A, Yang H*, Wang S.
LWT - Food Science and Technology,2017,79(-):428-436
2017年01月24日
Electrolysed water (EW) is an activated liquid with a high oxidation-reduction potential. EW causes oxidative damage to pathogenic microorganisms and as a result, may have utility in the food industry. The molecular mechanism of EW's action is not understood. In this study, we exposed Escherichia coli ATCC 25922 to a sub-lethal concentration of EW and examined structural and metabolic changes. Atomic force microscopy revealed that EW caused damage to E. coli membranes. To understand the metabolic responses to EW perturbations in of E. coli, multivariate data analysis of NMR spectroscopy demonstrated that EW significantly influenced the metabolic state. This included reducing nucleotide and amino acid biosynthesis, suppressing energy-associated metabolism, altering osmotic adjustment, and promoting fatty acid metabolism. The results enrich our understanding of E. coli metabolic changes caused by EW perturbation and support the effectiveness of the NMR metabolomics as a valuable tool to analyse and evaluate such a complex biological system.
关键词: 电解水, 大肠杆菌, 氧化应激, 核磁共振, 原子力显微镜;Electrolysed water, Escherichia coli, Oxidative stress, NMR, Atomic force microscopy
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【期刊论文】一种能够产生中性电解水的便携式电解消毒设备的开发
杨宏顺, Jufang Zhang , Shigang Zhou , Ronghua Chen , Hongshun Yang
LWT - Food Science and Technology,2017,82(1):207-215
2017年04月10日
The aim of this study was to develop and evaluate the characteristics and performance of a portable electrolytic sanitising unit. Free available chlorine (FAC), oxidation-reduction potential, and pH of electrolysed water were measured. Response surface methodology coupled with a Box-Behnken design was used to describe the input-output relationship and optimise FAC production. A partial catholyte solution was reintroduced to electrolysis for generating neutral electrolysed water. The result found that RuO2- IrO2/TiO2 electrode was very effective. A FAC concentration of 4 mg/L achieved >2 log CFU/mL reduction, while a FAC concentration of 40 mg/L achieved >6 log CFU/mL reduction in Escherichia coli O157:H7 and Listeria monocytogenes BAA-839. The developed sanitiser had a pH of 7.08 ± 0.08, and the commercial sanitiser had a pH of 3.77 ± 0.18. The developed sanitiser had similar bactericidal effects as the commercial sanitiser. The results revealed that the developed sanitising unit is promising for the control of foodborne pathogens.
关键词: 便携式消毒设备, 响应面优化设计, 杀菌活性, 大肠杆菌O157:H7型, 单核细胞增多性李斯特氏菌;Portable sanitising unit , Box-Behnken design, Bactericidal activity, Escherichia coli O157:H7, Listeria monocytogenes
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杨宏顺, Qin Liu , Chih Shen Cedric Tan , Hongshun Yang , *, Shifei Wang
LWT - Food Science and Technology,2017,79(-):594-600
2017年09月27日
本文评估了低浓度电解水(LcEW))(4 mg/L 游离有效氯 )温热处理清洗消毒技术,对有机西兰花安全和质量的影响。发现LcEW在50 oC温热处理情况下,对微生物及病原菌的杀灭能力最强。处理后的西兰花,抗氧化物含量、总酚水平以及铁离子还原力都维持不变,但是和未经过消毒处理的样品对比,氧自由基吸收能力升高。此外,温热处理提升了西兰花坚实度,这与果胶结构的改变相关。结果显示,温热处理引发了果胶链的拟定向和自发聚合。本研究显示,LcEW的温热处理能够在保证新鲜有机西兰花质量不打折扣的前提下,有效的减少微生物总数。
关键词: 消毒清洁, 果胶, 原子力显微镜, 抗氧化活性, 有机食品;Sanitisation, Pectin, Atomic force microscopy, Antioxidant activity, Organic food
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【期刊论文】低温冷萃结合纳米磁珠萃取里测定植物油脂中拟除虫菊酯
杨宏顺, Xi Yu , Hwee Chieh Ang, Hongshun Yang *, Chao Zheng , Yuquan Zhang
Food Control,-0001,():
暂无
关键词: 特征, 玉米油, 电子显微镜食品分析, 食品安全, 有害物质, 傅里叶变换光谱, 低温清除, 磁性固相萃取, 纳米颗粒, 纳米技术, 橄榄油, 最优化, 有机食品, 杀虫剂, 拟除虫菊酯, 快速检测, 大豆油, 合成, 植物油
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银建中, YI-FAN LIU, PENG LUO, QIN-QIN XU, EN-JUN WANG and JIAN-ZHONG YIN*
CELLULOSE CHEMISTRY AND TECHNOLOGY ,-0001,():
暂无
关键词: lignocellulose,, supercritical carbon dioxide,, pretreatment,, reducing sugars
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【期刊论文】Ophiopogonin D prevents H2O2-induced injury in primary human umbilical vein endothelial cells
刘畅, Qian, J., Jiang, F., Wang, B., Yu, Y., Zhang, X., Yin, Z., & Liu, C.
J. Ethnopharmacol. (2010), 128: 438-445.,-0001,():
暂无
关键词: Endothelial injury model,, Ophiopogonin D,, Oxidative stress,, Inflammation,, Apoptosis
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刘畅, Xu, Y., Chen, J., Yu, X., Tao, W., Jiang, F., Yin, Z., & Liu, C.
Inflamm. Res. (2010), 59: 871-877.,-0001,():
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刘畅, Yu, X., Tao, W., Jiang, F., Li, C., Lin, J., & Liu
Am. J. Hypertens. (2010), 23: 895-903.,-0001,():
暂无
关键词: celastrol,, vascular smooth muscle cells,, hypertension,, inflammation,, oxidative stress,, heme oxygenase-1
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【期刊论文】Ligustrazine improves atherosclerosis in rat via attenuation of oxidative stress
刘畅, Jiang, F., Qian, J., Chen, S., Zhang, W., & Liu, C.
Pharmaceutical Biology (2011), 49: 856-863.,-0001,():
暂无
关键词: Ligustrazine,, atherosclerosis,, oxidative stress,, dyslipidemia
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【期刊论文】PGC-1 coactivators in the control of energy metabolism
刘畅, Liu, C. & Lin, J.D.
Acta Biochimica et Biophysica Sinica (2011), 43: 248-257.,-0001,():
暂无
关键词: trans, c, r, i, p, t, ional coactivator,, metabolic syndrome,, PGC-1,, mitochondrial,, energy metabolism
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刘畅, Tao, W., Chen, S., Shi, G., Guo, J., Xu, Y., & Liu, C.
Hepatology (2011), 54: 1410-1420.,-0001,():
暂无
关键词: circadian clock,, metabolism,, BAF60a,, chromatin remodeling,, liver
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洪枫, Xiang Guo(郭香), Adnan Cavka, Leif J Jönsson and Feng Hong(洪枫)*
Microbial Cell Factories, 2013, 12: 93.,-0001,():
暂无
关键词: Bacterial cellulose,, Gluconacetobacter xylinus,, Norway spruce hydrolysate,, Detoxification methods
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【期刊论文】Production of bacterial cellulose and enzyme from waste fiber sludge
洪枫, Adnan Cavka, Xiang Guo(郭香), Shui-Jia Tang(唐水佳), Sandra Winestrand, Leif J. Jönsson, Feng Hong(洪枫)*
Biotechnology for Biofuels, 2013; 6: 25.,-0001,():
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关键词: Bacterial cellulose,, Gluconacetobacter xylinus,, Enzyme production,, Cellulase,, Trichoderma reesei,, Fiber sludge
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【期刊论文】Biotransformation of wheat straw to bacterial cellulose and its mechanism
洪枫, Lin Chen(陈琳), Feng Hong(洪枫)*, Xue-xia Yang(杨雪霞), Shi-fen Han(韩士芬)
Bioresource Technology, 2013, 135, 464-468,-0001,():
暂无
关键词: Bacterial cellulose,, Biorefinery,, Wheat straw,, Ionic liquid pretreatment,, 1-Allyl-3-methylimidazolium chloride (, [AMIM]Cl),
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洪枫, 谭玉静, 洪枫*, 邵志宇
中国生物工程杂,2007,27(4):126-131,-0001,():
细菌纤维素是一种天然的生物高聚物,具有生物活性、生物可降解性、生物适应性,具有独特的物理、化学和机械性能,例如高的结晶度、高的持水性、超细纳米纤维网络、高抗张强度和弹性模量等,因而成为近年来国际上新型生物医学材料的研究热点。概括细菌纤维素的性质、研究历史以及在生物医学材料上的应用,重点阐述细菌纤维素在组织工程支架、人工血管、人工皮肤和治疗皮肤损伤方面的应用以及当前研究现状。
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洪枫, Kun Zhong, Sheng-Yin Zhao, Leif J. Jönsson, Feng Hong*
Biocatalysis and Biotransformation. 2008, 26(4), 306-312,-0001,():
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关键词: Catechin,, biotransformation,, epigallocatechin,, epigallocatechin gallate,, Aspergillus niger,, induction
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洪枫, Kun Zhong(钟坤), Zhi-Yu Shao(邵志宇), Feng Hong(洪枫)*
Biotechnology Progress, 2008, 24(3), 583-587.,-0001,():
暂无
关键词: Catechin,, biotransformation,, epigallocatechin,, epigallocatechin gallate,, Aspergillus oryzae,, induction
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【期刊论文】GIS is required for trichome branching through GA signalling in Araidopsis
甘银波, An L, Zhou Z, Su S, Yan A, Gan Y*
Plant and Cell Physiology,-0001,():
暂无
关键词: GIS,, trichome,, GA
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【期刊论文】SPATULA Links Daytime Temperature and Plant Growth Rate.
甘银波, Sidaway-Lee K, Josse EM, Brown A, Gan Y, Halliday KJ, Graham IA, Penfield S.
Current Biology,-0001,():
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关键词: SPT,, temperature, growth
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