您当前所在位置: 首页 > 学者
为您找到 1 条相关结果
按相关度
  • 按相关度
  • 按时间
  • 按阅读量
  • 代表性成果优先

上传时间

2010年08月30日

【期刊论文】Genomic organization and expression analysis of Toll-like receptor 3 in grass carp (Ctenopharyngodon idella)

苏建国, Jianguo Su a, *, Songhun Jang b, Chunrong Yang a, Yaping Wang b, Zuoyan Zhu b

Fish & Shellfish Immunology 27(2009)433-439,-0001,():

摘要

investigate grass carp immune system responding to GCRV (grass carp reovirus) infection, the full-length cDNA sequence and genomic organization of grass carp TLR3 (CiTLR3) was identified and characterized. The full-length genome sequence of CiTLR3 is composed of 5668 nucleotides, including five exons and four introns. The full-length of CiTLR3 cDNA is 3681 bp in length and encodes a polypeptide of 904 amino acids with an estimated molecular mass of 102,765 Da and a predicted isoelectric point of 8.35. Analysis of the deduced amino acid sequence indicated that CiTLR3 has four main structural domains, including a signal peptide sequence, 14 LRR (leucine-rich repeat) motifs, a transmembrane region and a TIR (Toll/interleukin-1 receptor) domain. It is most similar to the crucian carp (Carassius auratus) TLR3 amino acid sequence with an identity of 99%. Quantitative RT-PCR analysis showed that CiTLR3 transcripts were significantly up-regulated starting at day 1 and continued through day 7 following GCRV infection (P<0.05). These data implied that CiTLR3 is involved in antiviral defense, provide molecular and functional information for grass carp TLR3, and implicate their role in mediating immune protection against grass carp viral diseases.

关键词: Ctenopharyngodon idella,, Toll-like receptor 3,, Genomic organization,, Gene cloning,, Grass carp reovirus

  • 26浏览

  • 0点赞

  • 0收藏

  • 0分享

  • 94下载

  • 0评论

  • 引用