- 0000年 18
- 期刊论文 18
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- Developmental & comparative immunology 4
- Fish & Shellfish Immunology 2
- Developmental & Comparative Immunology 2015; 48(1):86-94. 1
- Developmental & comparative immunology 1
- Fish & Shellfish Immunology 28(2010)712-718 1
- Fish & Shellfish Immunology 30 (2011) 936-943 1
- Fish &Shellfish Immunology 29(2010)349-355 1
- Fish & Shellfish Immunology 30 (2011) 317-323 1
- Fish & Shellfish Immunology 30 (2011) 45-50 1
- General and Comparative Endocrinology 134(2003)285-295 1
- PLoS One 1
- Scientific Reports 2015; 5: 12946. 1
- 苏建国 16
- Jianguo Su* 13
- Chunrong Yang 6
- Limin Peng 6
- Chunrong Yang 5
- Teng Huang 4
- Rongfang Zhang 4
- Qingmei Li 3
- Xiaohui Chen 3
- Youliang Rao 3
- Jie Dong 2
- Lijun Chen 2
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苏建国, Quanyuan Wan, Jianguo Su*
Scientific Reports 2015; 5: 12946.,-0001,():
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关键词: Grass carp (, Ctenopharyngodon idella), , RNA-seq, MiSeq, Alternative splice, Grass carp reovirus
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苏建国, Xueying Shang, Jianguo Su*, Quanyuan Wan, Juanjuan Su
Developmental & Comparative Immunology 2015; 48(1):86-94.,-0001,():
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关键词: Grass carp (, Ctenopharyngodon idella), , MDA5, CpA, DNA methylation, mRNA expression, Grass carp reovirus
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苏建国, Youliang Rao, Jianguo Su*, Chunrong Yang, Nana Yan, Xiaohui Chen and Xiaoli Feng
Cellular & Molecular Immunology 2015; 12(3): 342-353.,-0001,():
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关键词: grass carp (, Ctenopharyngodon idella), , grass carp reovirus, HMGBs, innate immunity, subcellular localization
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苏建国, Xiaoli Feng, Chunrong Yang, Yixuan Zhang, Limin Peng, Xiaohui Chen, Youliang Rao, Tianle Gu, Jianguo Su*
Developmental & comparative immunology,-0001,():
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关键词: Grass carp (, Ctenopharyngodon idella), , STING, PAMPs, Grass carp reovirus, Immune responses
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73浏览
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苏建国, Chunrong Yang, Qingmei Li, Jianguo Su*, Xiaohui Chen, Yaping Wang, Limin Peng
Developmental & comparative immunology,-0001,():
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关键词: Grass carp (, Ctenopharyngodon idella), , TRIF, Genomic structure, promoter activity, antiviral immunity, Grass carp reovirus
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31浏览
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苏建国, Chunrong Yang, Lijun Chen, Jianguo Su*, Xiaoli Feng, Youliang Rao
Fish & Shellfish Immunology,-0001,():
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关键词: Grass carp (, Ctenopharyngodon idella), , HMGB3, Grass carp reovirus, Innate immunity, Signal transduction
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18浏览
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【期刊论文】Protective roles of grass carp Ctenopharyngodon idella Mx isoforms against grass carp reovirus
苏建国, Limin Peng, Chunrong Yang, Jianguo Su*
PLoS One,-0001,():
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关键词: Grass carp (, Ctenopharyngodon idella), , Mx, Antiviral activity, Grass carp reovirus, Type I interferon
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32浏览
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苏建国, Youliang Rao, Jianguo Su*, Chunrong Yang, Limin Peng, Xiaoli Feng, Qingmei Li
Developmental & comparative immunology,-0001,():
暂无
关键词: Grass carp (, Ctenopharyngodon idella), , HMGB2, Grass carp reovirus, Innate immunity, Antiviral activity
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50浏览
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苏建国, Chunrong Yang, Limin Peng, Jianguo Su*
Developmental & comparative immunology,-0001,():
暂无
关键词: Grass carp (, Ctenopharyngodon idella), , HMGB1, PAMPs, mRNA expression, Cell proliferation, Grass carp reovirus
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35浏览
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苏建国, Quanyuan Wan, Jianguo Su*, Xiaohui Chen, Chunrong Yang
Developmental & comparative immunology,-0001,():
暂无
关键词: Grass carp (, Ctenopharyngodon idella), , IPS-1, Genetic polymorphism, Haplotype, Genomic organization, Grass carp reovirus
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苏建国, Chunrong Yang, Jianguo Su*, Qingmei Li, Rongfang Zhang, Youliang Rao
Fish & Shellfish Immunology,-0001,():
暂无
关键词: Grass carp (, Ctenopharyngodon idella), , Antiviral immunity, ADAR1, mRNA expression, Grass carp reovirus------WebKitFormBoundaryGeovirus
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苏建国, Jianfu Heng, Jianguo Su*, Teng Huang, Jie Dong, Lijun Chen
Fish & Shellfish Immunology 30 (2011) 45-50,-0001,():
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关键词: Grass carp (, Ctenopharyngodon idella), ,, TLR3,, Polymorphism,, Haplotype,, Grass carp reovirus
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26浏览
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苏建国, Jianguo Su*, Teng Huang, Chunrong Yang, Rongfang Zhang
Fish & Shellfish Immunology 30 (2011) 317-323,-0001,():
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关键词: Grass carp (, Ctenopharyngodon idella), ,, IPS-1,, Gene cloning,, mRNA expression,, Grass carp reovirus
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35浏览
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苏建国, Chunrong Yang, Jianguo Su*, Teng Huang, Rongfang Zhang, Limin Peng
Fish & Shellfish Immunology 30 (2011) 936-943,-0001,():
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关键词: Grass carp (, Ctenopharyngodon idella), , RIG-I, Gene cloning, mRNA expression, Grass carp reovirus
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苏建国, Jianguo Su*, Teng Huang, Jie Dong, Jianfu Heng, Rongfang Zhang, Limin Peng
Fish & Shellfish Immunology 28(2010)712-718,-0001,():
The cytoplasmic helicase protein MDA5 (melanoma-differentiation-associated gene-5) recognizes long molecules of viral double-stranded RNA (dsRNA) and single-stranded RNA with 50 triphosphate and mediates type I interferon secretion. In the present study, the first MDA5 gene in fish was cloned and characterized from grass carp Ctenopharyngodon idella. The full length of the C. idella MDA5 (CiMDA5) cDNA is 3233 nucleotides in length and encodes a polypeptide of 961 amino acids. The deduced amino acid sequence contained six main structural domains: a CARD (caspase activation and recruitment domain), a DEXDc (DEAD/DEAH box helicase domain), a ResIII (conserved restriction domain of bacterial type III restriction enzyme), two HELICc (helicase superfamily c-terminal domain) and an RD (regulatory domain). The CiMDA5 mRNAwas widespread expression in the tested tissues, was high level in spleen, skin and gill tissues, and was up-regulated by GCRV injection by semi-quantitative RT-PCR assay. The CiMDA5 transcripts in spleen were significantly up-regulated at 12 h (1.80 folds, P< 0.05), reached the crest at 24 h (7.48 folds, P<0.05), and then recovered to normal level at 48 h post-injection (P>0.05) of grass carp reovirus (GCRV). In liver, the temporal expression of CiMDA5 mRNA was significantly increased at 48 h (5.00 folds, P<0.05) and returned to control level at 72 h (P>0.05) after GCRV challenge. These data implied that CiMDA5 involved in the early stage of antiviral innate immune defense to GCRV in grass carp, and provided new insight into the evolution research of RLR (RIG-I like receptor) gene family.
关键词: Grass carp (, Ctenopharyngodon idella), ,, MDA5,, RLR,, Gene cloning,, mRNA expression,, ., Grass carp reovirus
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【期刊论文】Identification andexpressionprofilinganalysisofgrasscarp CtenopharyngodonidellaLGP2 cDNA
苏建国, TengHuang, JianguoSu*, JianfuHeng, JieDong, RongfangZhang, HuimingZhu
Fish &Shellfish Immunology 29(2010)349-355,-0001,():
LGP2 (laboratoryofgeneticsandphysiology2), ahomologueof RIG-I (Retinoicacidinduciblegene-I)and MDA5 (Melanomadifferentiationassociatedgene5)withouttheCARD (caspaseactivationandrecruitment domain)requiredforsignaling, playsapivotalroleinmodulatingsignalingby RIG-I andMDA5 for interferon (IFN)synthesis. Inthisstudy, anovel LGP2 genefromgrasscarp Ctenopharyngodonidella (designatedas CiLGP2) wasisolatedandcharacterized. Thefull-lengthcDNAof CiLGP2 wasof2920bpwith fiveinstability motifs (ATTTA). Theopenreadingframewasof2043bpandencodedapolypeptideof680aminoacids, including fivemainoverlappingstructuraldomains:twoDEXDc (DEAD/DEAHboxhelicasedomain), one ResIII (conservedrestrictiondomainofbacterialtypeIIIrestrictionenzyme), oneHELICc (helicase superfamilyc-terminaldomain)andoneRD (regulatorydomain). Therewasonemore a-helixintheRD, comparedwiththatinhuman. The CiLGP2 mRNAwasubiquitousexpressioninthetestedtissues, washigh levelinspleen, skin, heartandintestinetissues, andwasup-regulatedbygrasscarpreovirus (GCRV) injectionbysemi-quantitativeRT-PCR (sqRT-PCR)assay. The CiLGP2 expressioninspleenwassignificantly up-regulatedat12h (14.5 folds, P<0.05), reachedthecrestat24h (19.0 folds, P<0.05), andthendropped a littleat48h (10. 4folds)post-injectionofGCRVandkeptthislevelinthefollowingtestperiod (P<0.05). In liver, thetemporalexpressionof CiLGP2 mRNAwassignificantlyincreasedat24h (3.8 times, P<0.05), reachedpeakat 48h (10.7 times, P<0.05), andthendecreasedalittlebitat72h (5.8times, P<0.05) and keptthishighlevelbytheendofthetest (P<0.05). Theseresultscollectivelysuggestedthat CiLGP2 was a novelmemberofRLRgenefamily, engagingintheearlystageofantiviralinnateimmunedefenseingrass carp, andlaidthefoundationforthefurthermechanismresearchof LGP2 in fishes.
关键词: Grass carp (, Ctenopharyngodon idella), LGP2 Gene cloning mRNAexpression Grass carpreovirus
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林浩然, Dong Xiao , Hao-Ran Lin*
Comparative Biochemistry and Physiology Part A 134(2003)93-99,-0001,():
暂无
关键词: Grass carp (, Ctenopharyngodon idellus), , Cysteamine, Growth, Growth hormone, LHRH-A, RNAyDNA ratio, Somatostatininhibiting agent
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林浩然, Dong Xiao and Hao-Ran Lin*
General and Comparative Endocrinology 134(2003)285-295,-0001,():
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关键词: Grass carp (, Ctenopharyngodon idellus), , Cysteamine, Growth hormone, Growth, Hypothalamus plus pituitary fragments co-culture, Thyroid hormone, Administration in diet
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