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2017年03月10日

【期刊论文】核磁共振氢谱技术评估大肠杆菌对电解水的代谢反应

杨宏顺, Liu Q, Wu J, Lim ZY, Aggarwal A, Yang H*, Wang S.

LWT - Food Science and Technology,2017,79(-):428-436

2017年01月24日

摘要

Electrolysed water (EW) is an activated liquid with a high oxidation-reduction potential. EW causes oxidative damage to pathogenic microorganisms and as a result, may have utility in the food industry. The molecular mechanism of EW's action is not understood. In this study, we exposed Escherichia coli ATCC 25922 to a sub-lethal concentration of EW and examined structural and metabolic changes. Atomic force microscopy revealed that EW caused damage to E. coli membranes. To understand the metabolic responses to EW perturbations in of E. coli, multivariate data analysis of NMR spectroscopy demonstrated that EW significantly influenced the metabolic state. This included reducing nucleotide and amino acid biosynthesis, suppressing energy-associated metabolism, altering osmotic adjustment, and promoting fatty acid metabolism. The results enrich our understanding of E. coli metabolic changes caused by EW perturbation and support the effectiveness of the NMR metabolomics as a valuable tool to analyse and evaluate such a complex biological system.

关键词: 电解水, 大肠杆菌, 氧化应激, 核磁共振, 原子力显微镜;Electrolysed water, Escherichia coli, Oxidative stress, NMR, Atomic force microscopy

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2017年05月02日

【期刊论文】一种能够产生中性电解水的便携式电解消毒设备的开发

杨宏顺, Jufang Zhang , Shigang Zhou , Ronghua Chen , Hongshun Yang

LWT - Food Science and Technology,2017,82(1):207-215

2017年04月10日

摘要

The aim of this study was to develop and evaluate the characteristics and performance of a portable electrolytic sanitising unit. Free available chlorine (FAC), oxidation-reduction potential, and pH of electrolysed water were measured. Response surface methodology coupled with a Box-Behnken design was used to describe the input-output relationship and optimise FAC production. A partial catholyte solution was reintroduced to electrolysis for generating neutral electrolysed water. The result found that RuO2- IrO2/TiO2 electrode was very effective. A FAC concentration of 4 mg/L achieved >2 log CFU/mL reduction, while a FAC concentration of 40 mg/L achieved >6 log CFU/mL reduction in Escherichia coli O157:H7 and Listeria monocytogenes BAA-839. The developed sanitiser had a pH of 7.08 ± 0.08, and the commercial sanitiser had a pH of 3.77 ± 0.18. The developed sanitiser had similar bactericidal effects as the commercial sanitiser. The results revealed that the developed sanitising unit is promising for the control of foodborne pathogens.

关键词: 便携式消毒设备, 响应面优化设计, 杀菌活性, 大肠杆菌O157:H7型, 单核细胞增多性李斯特氏菌;Portable sanitising unit , Box-Behnken design, Bactericidal activity, Escherichia coli O157:H7, Listeria monocytogenes

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2017年05月02日

【期刊论文】酸性电解水温热处理对有机西兰花清洗消毒

杨宏顺, Qin Liu , Chih Shen Cedric Tan , Hongshun Yang , *, Shifei Wang

LWT - Food Science and Technology,2017,79(-):594-600

2017年09月27日

摘要

本文评估了低浓度电解水(LcEW))(4 mg/L 游离有效氯 )温热处理清洗消毒技术,对有机西兰花安全和质量的影响。发现LcEW在50 oC温热处理情况下,对微生物及病原菌的杀灭能力最强。处理后的西兰花,抗氧化物含量、总酚水平以及铁离子还原力都维持不变,但是和未经过消毒处理的样品对比,氧自由基吸收能力升高。此外,温热处理提升了西兰花坚实度,这与果胶结构的改变相关。结果显示,温热处理引发了果胶链的拟定向和自发聚合。本研究显示,LcEW的温热处理能够在保证新鲜有机西兰花质量不打折扣的前提下,有效的减少微生物总数。

关键词: 消毒清洁, 果胶, 原子力显微镜, 抗氧化活性, 有机食品;Sanitisation, Pectin, Atomic force microscopy, Antioxidant activity, Organic food

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2017年07月29日

【期刊论文】Draft Genome Sequence of Paenibacillus sp. Strain MY03, a Terrestrial Bacterium Capable of Degrading Multiple Marine- Derived Polysaccharides

韩文君, Huihui Liu, a, Yuanyuan Cheng, Jingyan Gu, Yanhui Wang, Junge Li, Fuchuan Li, Wenjun Han*

Genome Announcements,2017,5(29):e0078-17

2017年07月20日

摘要

he bacterium Paenibacillus sp. strain MY03, isolated from the root soil of cypress, can effectively degrade marine-derived polysaccharides such as agar, algi- nate, and chitin. Here, we present the draft genome sequence of MY03. Putative en- zymes, including 3 agarases, 1 alginate lyase, and 1 chitinase, were found

关键词: Agarase,, Alginate lyase,, Chintinase,, Polysaccharide-degrading eznymes,,

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2019年05月03日

【期刊论文】Stability of the traveling waves for the derivative Schrödinger equation in the energy space

Changxing Miao, Xingdong Tang, Guixiang Xu

Calculus of Variations and PDEs,2017,56-54(1):1-48

2017年08月30日

摘要

In this paper, we continue the study of the dynamics of the traveling waves for nonlinear Schrödinger equation with derivative (DNLS) in the energy space. Under some technical assumptions on the speed of each traveling wave, the stability of the sum of two traveling waves for DNLS is obtained in the energy space by Martel–Merle–Tsai’s analytic approach in Martel et al. (Commun Math Phys 231(2):347–373, 2002, Duke Math J 133(3):405–466, 2006). As a by-product, we also give an alternative proof of the stability of the single travelingwave in the energy space in Colin andOhta (Ann InstHenri Poincaré Anal Non Linéaire 23(5):753–764, 2006), where Colin and Ohta made use of the concentration compactness argument.

关键词: traveling waves ,, Schrödinger equation with derivative,, concentration compactness argument.,

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