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2017年05月02日

【期刊论文】采用豌豆蛋白研发一种适合蛋奶素食主义者使用的无蛋蛋糕

杨宏顺, Muyang Lin , Siang Hong Tay , Hongshun Yang, *, Bao Yang , Hongliang Li

Food Hydrocolloids,2017,69(-):440-449

2017年10月03日

摘要

Cakes are important confectionery and are enjoyed socially within groups. Eggs are important components of cakes, providing emulsification, flavour, colour, and many other properties. However, because of various health concerns and religious beliefs, some consumers cannot enjoy traditional cakes made with eggs. In the current study, isolated pea protein (PPI), xanthan gum (XN), and emulsifier mixtures were investigated to prepare eggless cakes and their roles were determined. The physicochemical properties of the batter and final cake products, the microstructure of the final cakes, and structural properties of starches and glutens at the meso-scale were characterised. The eggless cake recipe containing PPI, 0.1% XN and 1% soy lecithin (SL) was found to be close to the control traditional cakes in terms of specific gravity (1.01 vs. 1.03), crumb colour (a* at 0.09 vs. 0.04), and crumb pore properties (average pore area both 0.20 mm2). Cakes with formulation R4 were most similar to the control cakes with respect to the 1047/1022 relative intensity of the IR spectra of starches (both 0.55) and the nanostructures of glutenins. Therefore, formulation PPIþ0.1% XNþ 1% SL was considered as a potential candidate recipe for substituting eggs in cakes.

关键词: 蛋糕, 豌豆蛋白, 黄原胶, 乳化剂, 傅里叶变换红外光谱, 原子力显微镜;Cake, Pea protein, Xanthan gum, Emulsifier, Fourier transform infrared spectroscopy (FTIR), Atomic force microscopy (AFM)

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2018年05月29日

【期刊论文】Characterisation of rheology and microstructures of κ-carrageenan in ethanol-water mixtures

Zhi Yang, Huijuan Yang, Hongshun Yang

Food Research International,2018,107(-):738-746

2018年03月04日

摘要

The effects of ethanol (up to 20 wt%) on the rheological properties and structural characteristics of κ-carrageenan gel were investigated by Field Emission Scanning Electron Microscopy (FESEM) and Small Angle X-ray Scattering (SAXS). Both the sol-gel and gel-sol transition temperatures shifted to higher degree (from 36.8 ± 0.5 to 52.5 ± 1.4 °C and from 51.2 ± 0.6 to 67.0 ± 0.5 °C, respectively) upon 20 wt% ethanol addition (P < 0.05). The critical relaxation exponent n and the critical gel strength Sg obtained from Winter-Chambon criterion decreased and increased, respectively as the ethanol concentration increased. The κ-carrageenan gel was formed due to the formation of fibrillar networks, and the fibrillar density increased upon ethanol addition via FESEM. Moreover, upon 20 wt% ethanol addition, the average radius of gyration of κ-carrageenan strand increased from 1.18 ± 0.03 of control to 1.55 ± 0.02 nm by SAXS. A mechanism underlying the effect of ethanol on the κ-carrageenan gelation was proposed based on coil to double helix transition followed by the helix aggregation.

关键词: Polysaccharide, Sol-gel transition, Rheology, Solvent quality, Network strength, Small angle X-ray scattering

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2019年05月03日

【期刊论文】Sobolev space adapted to the Schrodinger operator with inverse-square potential

苗长兴:R.Killip,C. Miao, M.Visan,J.Zhang.,J.Zheng

Mathematische Zeitschrift,2018,288(4):1273–1298

2018年01月30日

摘要

We study the $L^p$-theory for the Schr\"odinger operator with critical rough potential of $a|x|^{-2}$ type. The developed harmonic analysis tools, such as multiplier estimate, Littlewood-Paley theory and the equivalence of Sobolev norms, will be employed to study the scattering theory of energy-critical defocusing nonlinear Schr\"odinger equation with the inverse-square potential. These tools in $L^p$-theory are new and the range of $p$ depends on the parameter $a$, which is different from the classical theory. The main difficulty is raised from the failure of Gaussian boundedness of the heat kernel associated with the operator $P_a=-\Delta+a|x|^{-2}$ when $a$ is negative.

关键词: Riesz transforms · Inverse-square potential · Littlewood–Paley theory · Mikhlin multiplier theorem ·

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2019年11月17日

【期刊论文】Transcriptome analysis of two inflorescence branching mutants reveals cytokinin is an important regulator in controlling inflorescence architecture in the woody plant Jatropha curcas

Mao-Sheng Chen, Mei-Li Zhao, Gui-Juan Wang, Hui-Ying He, Xue Bai, Bang-Zhen Pan, Qiantang Fu, Yan-Bin Tao, Mingyong Tang, Jorge Martínez-Herrera, Zeng-Fu Xu, Mao-Sheng Chen, Mei-Li Zhao, Gui-Juan Wang, Hui-Ying He, Xue Bai, Bang-Zhen Pan, Qiantang Fu, Yan-Bin Tao, Mingyong Tang, Jorge Martínez-Herrera, Zeng-Fu Xu

BMC Plant Biology,2019,19(1):468

2019年11月04日

摘要

In higher plants, inflorescence architecture is an important agronomic trait directly determining seed yield. However, little information is available on the regulatory mechanism of inflorescence development in perennial woody plants. Based on two inflorescence branching mutants, we investigated the transcriptome differences in inflorescence buds between two mutants and wild-type (WT) plants by RNA-Seq to identify the genes and regulatory networks controlling inflorescence architecture in Jatropha curcas L., a perennial woody plant belonging to Euphorbiaceae. Two inflorescence branching mutants were identified in germplasm collection of Jatropha. The duo xiao hua (dxh) mutant has a seven-order branch inflorescence, and the gynoecy (g) mutant has a three-order branch inflorescence, while WT Jatropha has predominantly four-order branch inflorescence, occasionally the three- or five-order branch inflorescences in fields. Using weighted gene correlation network analysis (WGCNA), we identified several hub genes involved in the cytokinin metabolic pathway from modules highly associated with inflorescence phenotypes. Among them, Jatropha ADENOSINE KINASE 2 (JcADK2), ADENINE PHOSPHORIBOSYL TRANSFERASE 1 (JcAPT1), CYTOKININ OXIDASE 3 (JcCKX3), ISOPENTENYLTRANSFERASE 5 (JcIPT5), LONELY GUY 3 (JcLOG3) and JcLOG5 may participate in cytokinin metabolic pathway in Jatropha. Consistently, exogenous application of cytokinin (6-benzyladenine, 6-BA) on inflorescence buds induced high-branch inflorescence phenotype in both low-branch inflorescence mutant (g) and WT plants. These results suggested that cytokinin is an important regulator in controlling inflorescence branching in Jatropha. In addition, comparative transcriptome analysis showed that Arabidopsis homologous genes Jatropha AGAMOUS-LIKE 6 (JcAGL6), JcAGL24, FRUITFUL (JcFUL), LEAFY (JcLFY), SEPALLATAs (JcSEPs), TERMINAL FLOWER 1 (JcTFL1), and WUSCHEL-RELATED HOMEOBOX 3 (JcWOX3), were differentially expressed in inflorescence

关键词: Transcriptome, inflorescence branching, cytokinin

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2020年04月20日

【期刊论文】Exogenous gene can be expressed by a recombinant Bombyx mori cypovirus

Rui Guo, Guangli Cao, Renyu Xue, Dhiraj Kumar, Fei Chen, Wei Liu, Yue Jiang, Yahong Lu, Liyuan Zhu, Zi Liang, Sunlan Kuang, Xiaolong Hu, Chengliang Gong

Applied Microbiology and Biotechnology,2018,102(3):1367-1379

2018年02月08日

摘要

Bombyx mori cypovirus (BmCPV) is one of the major viral pathogen for silkworm, and the genome of BmCPV is composed of 10 dsRNA segments. As construction system of recombinant BmCPV (rBmCPV) is scanty, researchers achieved little progress in studying gene function of BmCPV in recent decades. Here, 10 recombinant plasmids with a full-length cDNA of viral genome segments S1-S10 containing T7 promoter were constructed. After cotransfecting the BmN cells with the mixture of 10 in vitrotranscribed RNAs, pathological changes were observed. Real-time PCR and Western blot showed viral gene vp1 and structural proteins were expressed. It is found the genome of the rBmCPV is composed of 10 dsRNA segments similar to those of wild-type BmCPV. Moreover, viral particles and polyhedron with virions can be generated in the cotransfected cells and the injected silkworm midguts. These findings confirmed the formation of infective rBmCPV. Additionally, we found viable rBmCPV was generated by cotransfecting the mixture of in vitro-transcribed S1-S9 RNAs into the cultured cells, confirming polh was not essential for BmCPV replication. Moreover, an infectious rBmCPV expressing the DsRed protein was constructed based on this system. Further investigation showed S2 and S7 segments are indispensible for viral proliferation. Our findings demonstrated the construction system of rBmCPV can be utilized for exploring viral replication and pathogenesis, and investigated method for constructing rBmCPV will certainly facilitate developing novel biopesticides and expressing exogenous gene in the midgut of silkworm.

关键词: Bombyx mori, Cypovirus, Recombinant virus, In vitro-transcribed RNAs, Exogenous gene

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2020年04月20日

【期刊论文】Uncovering the immune responses of Apis mellifera ligustica larval gut to Ascosphaera apis infection utilizing transcriptome sequencing

Dafu Chen, Rui Guo, Xijian Xu, Cuiling Xiong, Qin Liang, Yanzhen Zheng, Qun Luo, Zhaonan Zhang, Zhijian Huang, Dhiraj Kumar, Weijun Xi, Xuan Zou, Min Liu

Gene,2017,621(1):40-50

2017年07月20日

摘要

Honeybees are susceptible to a variety of diseases, including chalkbrood, which is capable of causing huge losses of both the number of bees and colony productivity. This research is designed to characterize the transcriptome profiles of Ascosphaera apis-treated and un-treated larval guts of Apis mellifera ligustica in an attempt to unravel the molecular mechanism underlying the immune responses of western honeybee larval guts to mycosis. In this study, 24, 296 and 2157 genes were observed to be differentially expressed in A. apis-treated Apis mellifera (4-, 5- and 6-day-old) compared with un-treated larval guts. Moreover, the expression patterns of differentially expressed genes (DEGs) were examined via trend analysis, and subsequently, gene ontology analysis and KEGG pathway enrichment analysis were conducted for DEGs involved in up- and down-regulated profiles. Immunityrelated pathways were selected for further analysis, and our results demonstrated that a total of 13 and 50 DEGs were annotated in the humoral immune-related and cellular immune-related pathways, respectively. Additionally, we observed that many DEGs up-regulated in treated guts were part of cellular immune pathways, such as the lysosome, ubiquitin mediated proteolysis, and insect hormone biosynthesis pathways and were induced by A. apis invasion. However, more down-regulated DEGs were restrained. Surprisingly, a majority of DEGs within the Toll-like receptor signaling pathway, and the MAPK signaling pathway were up-regulated in treated guts, while all but two genes involved in the NF-κB signaling pathway were down-regulated, which suggested that most genes involved in humoral immune-related pathways were activated in response to the invasive fungal pathogen. This study's findings provide valuable information regarding the investigation of the molecular mechanism of immunity defenses of A. m. ligustica larval guts to infection with A. apis.

关键词: Immune response, Apis mellifera ligustica, Larval gut, Ascosphaera apis, RNA-seq, Transcriptome

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2020年04月20日

【期刊论文】Transcriptomic investigation of immune responses of the Apis cerana cerana larval gut infected by Ascosphaera apis

Rui Guo, Dafu Chen, Qingyun Diao, Cuiling Xiong, Yanzhen Zheng, Chunsheng Hou

Journal of Invertebrate Pathology,2019,166(1):170210-170

2019年09月08日

摘要

Chalkbrood is the most common fungal disease in honeybees. The objective of this study was to reveal immune responses in the Apis cerana cerana larval gut following Ascosphaera apis invasion. Combining a previously assembled transcriptome of A. c. cerana larval gut and the high-throughput sequencing data obtained in this study, 6152 differentially expressed genes (DEGs) were clustered into eight profiles. Trend analysis showed three significant up-regulated profiles (p ≤ 0.05) and three down-regulated profiles. Gene Ontology (GO) term analysis suggested that DEGs within significant up-regulated and down-regulated clusters were enriched in 46 and 38 functional groups, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis indicated a majority of DEGs were involved in ribosome structure or function, carbon metabolism, biosynthesis of amino acids, and oxidative phosphorylation. In addition, 142 and 14 DEGs were annotated in the cellular immune- and humoral immune-related pathways, respectively. Further investigation indicated that DEGs up-regulated in cellular immune and humoral immune pathways outnumbered those that were downregulated. Moreover, immune responses of A. c. cerana and Apis mellifera ligustica larvae were compared and studied to decipher resistance of eastern honeybee larvae to A. apis. These results demonstrated that a large number of genes involved in immunity-related pathways were activated by A. apis. Our findings provided valuable information for elucidating the molecular mechanisms underlying immune responses of A. c. cerana larvae to A. apis infection and pathogen-host interactions during chalkbrood infection.

关键词: Chalkbrood, Apis cerana cerana, Larval gut, Ascosphaera apis, Immune response, Transcriptome

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2020年04月21日

【期刊论文】Exogenous gene can be integrated into Nosema bombycis genome by mediating with a non-transposon vector

Rui Guo, Guangli Cao, Yahong Lu, Renyu Xue, Dhiraj Kumar, Xiaolong Hu, Chengliang Gong

Parasitology Research,2016,115(8):3093-3098

2016年08月08日

摘要

Nosema bombycis, a microsporidium, is a pathogen of pebrine disease of silkworms, and its genomic DNA sequences had been determined. Thus far, the research of gene functions of microsporidium including N. bombycis cannot be performed with gain/loss of function. In the present study, we targeted to construct transgenic N. bombycis. Therefore, hemocytes of the infected silkworm were transfected with a nontransposon vector pIZT/V5-His vector in vivo, and the blood, in which the hemocyte with green fluorescence could be observed, was added to the cultured BmN cells. Furthermore, normal BmN cells were infected with germinated N. bombycis, and the infected cells were transfected with pIZT/V5-His. Continuous fluorescence observations exposed that there were N. bombycis with green fluorescence in some N. bombycis-infected cells, and the extracted genome from the purified N. bombycis spore was used as templates. PCR amplification was carried out with a pair of primers for specifically amplifying the green fluorescence protein (GFP) gene; a specific product representing the gfp gene could be amplified. Expression of the GFP protein through Western blotting also demonstrated that the gfp gene was perfectly inserted into the genome of N. bombysis. These results illustrated that exogenous gene can be integrated into N. bombycis genome by mediating with a non-transposon vector. Our research not only offers a strategy for research on gene function of N. bombycis but also provides an important reference for constructing genetically modified microsporidium utilized for biocontrol of pests.

关键词: Transgenic Nosema bombycis, Bombyx mori, pIZT/, V5-His vector, BmN cells, Transfection, Fluorescence

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