您当前所在位置: 首页 > 学者
为您找到 3 条相关结果
按相关度
  • 按相关度
  • 按时间
  • 按阅读量
  • 代表性成果优先

上传时间

2020年05月06日

【期刊论文】A comprehensive transcriptome data of normal and Nosema ceranae-stressed midguts of Apis mellifera ligustica workers

Huazhi Chen, Yu Du, Cuiling Xiong, Yanzhen Zheng, Dafu Chen, Rui Guo

Data in Brief,2019,26(1):1-5

2019年08月22日

摘要

Honeybees are pivotal pollinators of crops and wild flora, and of great importance in supporting critical ecosystem balance. Nosema ceranae, a unicellular fungal parasite that infects midgut epithelial cells of honeybees, can dramatically reduce honeybee population and productivity. Here, midguts of Apis mellifera ligustica workers at 7 d and 10 d post inoculation (dpi) with sucrose solution (Ac7CK and Ac10CK) and midguts at 7 dpi and 10 dpi with sucrose solution containing N. ceranae spores (Ac7T and Ac10T) were sequenced using strand-specific cDNA library construction and next-generation sequencing. A total of 1956129858 raw reads were gained in this article, and after quality control, 1946489304 high-quality clean reads with a mean Q30 of 93.82% were obtained. The rRNA-removed clean reads were then aligned to the reference genome of Apis mellifera with TopHat2. For more insight please see “Genome-wide identification of long non-coding RNAs and their regulatory networks involved in Apis mellifera ligustica response to Nosema ceranae infection” [1]. Raw data were deposited in NCBI Sequence Read Archive (SRA) database under the BioProject number PRJNA406998. These data can be used for comparative analysis to identify differentially expressed coding RNAs and non-coding RNAs involved in host responses to N. ceranae stress, and for investigation of molecular mechanisms regulating N. ceranae-response.

关键词: Apis mellifera ligustica, Nosema ceranae, Midgut, Transcriptome

  • 6浏览

  • 0点赞

  • 0收藏

  • 0分享

  • 0下载

  • 0评论

  • 引用

上传时间

2020年05月06日

【期刊论文】MicroRNA dataset of normal and Nosema ceranae-infected midguts of Apis cerana cerana workers

Yu Du, Dingding Zhou, Huazhi Chen, Cuiling Xiong, Yanzhen Zheng, Dafu Chen, Rui Guo

Data in Brief,2019,26(1):1-6

2019年09月14日

摘要

N osema ceranae is a widespread fungal pathogen of honeybees, which is infective to all castes in the colony, including queens, drones and workers. Nosemosis caused by N. ceranae poses a big challenge for apiculture all over the world. In this articleHere, midguts of normal and N. ceranae-infected Apis cerana cerana workers at 7 (10) days post infection (dpi) were sequenced utilizing small RNA sequencing (sRNA-seq). Totally, 150.54 Mb raw reads were produced in this article, and 144.26 Mb high-quality clean reads with a mean ratio of 95.83% were obtained after strict filtering and quality control. For more insight please see “Comparative identification of microRNAs in Apis cerana cerana workers' midguts responding to Nosema ceranae invasion” [1]. Raw data are available in NCBI Sequence Read Archive (SRA) database under the BioProject number PRJNA487111. Our data can be used for investigating differentially expressed microRNAs (miRNAs) and piRNAs and their regulatory roles engaged in A. c. cerana response to N. ceranae infection, and for offering potential candidates for uncovering the molecular mechanisms regulating eastern honeybee-microsporidian interactions.

关键词: Apis cerana cerana, Nosema ceranae, Midgut, MicroRNA, Transcriptome

  • 13浏览

  • 0点赞

  • 0收藏

  • 0分享

  • 0下载

  • 0评论

  • 引用

上传时间

2020年05月06日

【期刊论文】Transcriptome data of control and Ascosphaera apis infected Apis mellifera ligustica larval guts

Huazhi Chen, Yu Du, Zhiwei Zhu, Cuiling Xiong, Yanzhen Zheng, Dafu Chen, Rui Guo

Data in Brief,2020,29(1):1-4

2020年02月08日

摘要

Ascosphaera apis is an obligate fungal pathogen of honeybee larvae that leads to chalkbrood, which causes heavy losses for the apiculture in China and many other countries. In this article, guts of 4-, 5-, 6-day-old Apis mellifera ligustica larvae challenged by A. apis (AmT1, AmT2, AmT3) and normal 4-day-old larval guts (AmCK) were sequenced using next-generation sequencing technology. On average, 29196197, 28690943, 29779715 and 30496725 raw reads were yielded from these four groups; an average of 29540895 clean reads were obtained after quality control. In addition, the mapping ratio of clean reads in treatment and control groups to the Apis mellifera genome were over 97.16%. For more insight please see “Uncovering the immune responses of Apis mellifera ligustica larval gut to Ascosphaera apis infection utilizing transcriptome sequencing” [1]. The raw data were submitted to the National Centre for Biotechnology Information (NCBI) Sequence Read Archive (SRA) database under accession numbers: SRR4084091, SRR4084092, SRR4084095, SRR4084096, SRR4084097, SRR4084098, SRR4084099, SRR4084100, SRR4084101, SRR4084102, SRR4084093, SRR4084094.

关键词: western honeybee, Apis mellifera ligustica, Ascosphaera apis, larvae, gut, transcriptome

  • 5浏览

  • 0点赞

  • 0收藏

  • 0分享

  • 0下载

  • 0评论

  • 引用