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2018年05月29日

【期刊论文】Characterisation of rheology and microstructures of κ-carrageenan in ethanol-water mixtures

Zhi Yang, Huijuan Yang, Hongshun Yang

Food Research International,2018,107(-):738-746

2018年03月04日

摘要

The effects of ethanol (up to 20 wt%) on the rheological properties and structural characteristics of κ-carrageenan gel were investigated by Field Emission Scanning Electron Microscopy (FESEM) and Small Angle X-ray Scattering (SAXS). Both the sol-gel and gel-sol transition temperatures shifted to higher degree (from 36.8 ± 0.5 to 52.5 ± 1.4 °C and from 51.2 ± 0.6 to 67.0 ± 0.5 °C, respectively) upon 20 wt% ethanol addition (P < 0.05). The critical relaxation exponent n and the critical gel strength Sg obtained from Winter-Chambon criterion decreased and increased, respectively as the ethanol concentration increased. The κ-carrageenan gel was formed due to the formation of fibrillar networks, and the fibrillar density increased upon ethanol addition via FESEM. Moreover, upon 20 wt% ethanol addition, the average radius of gyration of κ-carrageenan strand increased from 1.18 ± 0.03 of control to 1.55 ± 0.02 nm by SAXS. A mechanism underlying the effect of ethanol on the κ-carrageenan gelation was proposed based on coil to double helix transition followed by the helix aggregation.

关键词: Polysaccharide, Sol-gel transition, Rheology, Solvent quality, Network strength, Small angle X-ray scattering

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2019年01月03日

【期刊论文】Method and Application of Endogenous Neurotransmitter Regulation Theory

Zuodong Sun, Bing Lu, Yuting Sun, Aili Wang, Aobo Dong, Wenhua Wang, Wuyi Sun

viXra.org,2018,1802(2):1802-1810

2018年03月13日

摘要

[Objective] Verification and application of the theory of brain cell activation. [Methods] Applying Transcranial magnetoelectric encephalopathy treatment instrument to the treatment of Neurodegenerative disease. including but not limited to: Parkinson's disease and Alzheimer's disease. [Results] Transcranial magnetoelectric encephalopathy treatment instrument,its double - center, randomized, double - blind clinical trial has been carried out at the national clinical trial base,it proves that the instrument is safe and effective. This instrument is especially suitable for the treatment of the following diseases: 1. It is suitable for the treatment of mild to moderate Parkinson's disease and can significantly improve resting tremor, rigidity, bradykinesia in patients with Parkinson’s disease and other symptoms. 2. It is suitable for the treatment of mild and moderate Alzheimer's disease and vascular dementia and can improve the mental state, cognitive behavior and self-care ability of daily life. 3. It is suitable for the treatment of mild to moderate depression and can obviously improve the main symptoms of depression, sleep disorder, anxiety and so on. 4. It is suitable for the treatment of cerebral apoplexy sequelae, cerebrovascular dementia and brain atrophy, it can activate the brain cells in the state of inhibition and mobilize the potential energy of the brain. [Conclusions] The theory of brain cell activation,It is applicable to encephalopathy and not limited to encephalopathy,according to this theory, the subject of "physical disease science" can be created.

关键词: The theory of brain cell activation, Ca2+, channel, Transcranial magnetoelectric, Parkinson', s disease

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2019年05月03日

【期刊论文】Sobolev space adapted to the Schrodinger operator with inverse-square potential

苗长兴:R.Killip,C. Miao, M.Visan,J.Zhang.,J.Zheng

Mathematische Zeitschrift,2018,288(4):1273–1298

2018年01月30日

摘要

We study the $L^p$-theory for the Schr\"odinger operator with critical rough potential of $a|x|^{-2}$ type. The developed harmonic analysis tools, such as multiplier estimate, Littlewood-Paley theory and the equivalence of Sobolev norms, will be employed to study the scattering theory of energy-critical defocusing nonlinear Schr\"odinger equation with the inverse-square potential. These tools in $L^p$-theory are new and the range of $p$ depends on the parameter $a$, which is different from the classical theory. The main difficulty is raised from the failure of Gaussian boundedness of the heat kernel associated with the operator $P_a=-\Delta+a|x|^{-2}$ when $a$ is negative.

关键词: Riesz transforms · Inverse-square potential · Littlewood–Paley theory · Mikhlin multiplier theorem ·

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2020年04月20日

【期刊论文】Exogenous gene can be expressed by a recombinant Bombyx mori cypovirus

Rui Guo, Guangli Cao, Renyu Xue, Dhiraj Kumar, Fei Chen, Wei Liu, Yue Jiang, Yahong Lu, Liyuan Zhu, Zi Liang, Sunlan Kuang, Xiaolong Hu, Chengliang Gong

Applied Microbiology and Biotechnology,2018,102(3):1367-1379

2018年02月08日

摘要

Bombyx mori cypovirus (BmCPV) is one of the major viral pathogen for silkworm, and the genome of BmCPV is composed of 10 dsRNA segments. As construction system of recombinant BmCPV (rBmCPV) is scanty, researchers achieved little progress in studying gene function of BmCPV in recent decades. Here, 10 recombinant plasmids with a full-length cDNA of viral genome segments S1-S10 containing T7 promoter were constructed. After cotransfecting the BmN cells with the mixture of 10 in vitrotranscribed RNAs, pathological changes were observed. Real-time PCR and Western blot showed viral gene vp1 and structural proteins were expressed. It is found the genome of the rBmCPV is composed of 10 dsRNA segments similar to those of wild-type BmCPV. Moreover, viral particles and polyhedron with virions can be generated in the cotransfected cells and the injected silkworm midguts. These findings confirmed the formation of infective rBmCPV. Additionally, we found viable rBmCPV was generated by cotransfecting the mixture of in vitro-transcribed S1-S9 RNAs into the cultured cells, confirming polh was not essential for BmCPV replication. Moreover, an infectious rBmCPV expressing the DsRed protein was constructed based on this system. Further investigation showed S2 and S7 segments are indispensible for viral proliferation. Our findings demonstrated the construction system of rBmCPV can be utilized for exploring viral replication and pathogenesis, and investigated method for constructing rBmCPV will certainly facilitate developing novel biopesticides and expressing exogenous gene in the midgut of silkworm.

关键词: Bombyx mori, Cypovirus, Recombinant virus, In vitro-transcribed RNAs, Exogenous gene

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