全文从直肠癌的淋巴引流途径与转移规律、淋巴结清扫、对全直肠系膜切除术 ( TME) 的认识、直肠癌术后局部复发形式和术前、术中对淋巴转移的判定五方面进行了综述分析, 认为目前阶段缺少有效、准确、简便的判定淋巴转移的方法, 直肠癌扩大根治术现阶段应有积极的意义。鉴于诸位学者在过去的科研临床工作中, 对直肠癌的临床病理生物学特性有一些可指导手术的经验积累与认识, 为避免盲目扩大, 作者提出选择性保留盆腔植物神经的功能性 直肠癌扩大根治术。

Aim: Observing the Impact of arsenic trioxide (As2O3) on LS-174T cell (colorectal carcinoma cell lines) and the activity of telomerase. Methods: Using the methods of electron microscope (EMS), PCR-Elisa, flow cytometry (FCM) and MTT in vitro (cell culture). Results: (1) With the increasing of concentration of As2O3, the ratio of living cells decreased significantly, IC50=5.23μg/ml; (2) Impact of As2O3 on cell morphology: In experimental group, cells turned round, cell membrane shrunk and nucleus concentrated at karyotheca; (3) Apoptosis curve appeared after 24h, cells turned to apoptosis with time-dependent manner; (4) As2O3 inhibited the activity of telomerase of cell extraction obviously with concentration-dependent and time-dependent manner after 24 h, 48 h and 72 h respectively. Conclusion: From the experiment in vitro, we can see that As2O3 inhibit LS-174T cell growth mainly by inducing apoptosis, partly by the inhibition of telomerase activity.

Objective: Inhibition mechanism of telomerase inhibitor phosphorothioate antisense oligodeoxynucleotides (PS-ODN) on colorectal cancer cells is probed through cells culture and experimental observation of inhibition function of PS-ODN on LS-174T cells of colorectal cancer. Methods: To induce cells to die by adopting anti-sense technology to block telomerase RNA at reverse transcriptase level, and to evaluate the inhibition effect by means of cell clone forming、growth curve、electron microscope、 FCM、PCR-Elisa、etc. Results: The best dosage of PS-ODN was 10 μ mol/Ls. After ten days’ inhibition, the collective forming rate of LS-174T cells in group PS-ODN was obviously lower than that of those in group CPS-ODN and the contrast group ( P<0.01); The cells in group PS-ODN have obvious morphology change; After 72 hours’ inhibition, the cells in group PS-ODN show apoptosis peak, but this doesn’t happen in contrast group; telomerase activity of group PS-ODN was much lower than that of those in group CPS-ODN and the contrast group( P <0.01). Conclusions: Peculiar polynucleotide sequence can inhibit telomerase activity, and induce cells to wither and die. The treatment tactics of malignant tumours induced by telomerase activity is very promising.