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饶平凡, Tianbao Chen, , Anthony J. Bjourson, Stephen McClean, David F. Orr, Edmund J. O’Kane, Pingfan Rao, Chris Shaw
T. Chen et al. Peptides 24 (2003) 853-861,-0001,():
-1年11月30日
Using
Amphibian,
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饶平凡, Shaoyun
S. Wang et al. Biochemical and Biophysical Research Communications 327 (2005) 820-827,-0001,():
-1年11月30日
A novel lysozyme exhibiting antifungal activity and with a molecular mass of 14.4 kDa in SDS–polyacrylamide gel electrophoresis was isolated from mung bean (Phaseolus mungo) seeds using a procedure that involved aqueous extraction, ammonium sulfate precipitation, ion exchange chromatography on CM-Sephadex, and high-performance liquid chromatography on POROS HS-20. Its N-terminal sequence was very different from that of hen egg white lysozyme. Its pI was estimated to be above 9.7. The specific activity of the lysozyme was 355 U/mg at pH 5.5 and 30℃. The lysozyme exhibited a pH optimum at pH 5.5 and a temperature optimum at 55℃. It is reported herein, for the first time, that a novel plant lysozyme exerted an antifungal action toward Fusarium oxysporum, Fusarium solani, Pythium aphanidermatum, Sclerotium rolfsii, and Botrytis cinerea, in addition to an antibacterial action against Staphylococcus aureus.
Mung
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饶平凡, Tianbao
T. Chen et al. Regulatory Peptides 116 (2003) 147-154,-0001,():
-1年11月30日
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Amphibian,
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饶平凡, Tianbao
T. Chen et al. Regulatory Peptides 117 (2004) 25-32,-0001,():
-1年11月30日
Tryptophyllins
Peptide,
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饶平凡, Martin
M. O'Rourke et al. Regulatory Peptides 121 (2004) 65-72,-0001,():
-1年11月30日
Structural homologues of vertebrate regulatory peptides found in defensive skin secretions of anuran amphibians often display enhanced bioactivity and receptor binding when compared with endogenous mammalian peptide ligands. Maximakinin, a novel N-terminally extended bradykinin (DLPKINRKGPRPPGFSPFR) from the skin venom of a Chinese toad (Bombina maxima), displays such activity enhancement when compared with bradykinin but is additionally highly selective for mammalian arterial smooth muscle bradykinin receptors displaying a 50-fold increase in molar potency in this smooth muscle type. In contrast, a 100-fold decrease in molar potency was observed at bradykinin receptors in intestinal and uterine smooth muscle preparations. Maximakinin has thus evolved as a ‘‘smart" defensive weapon in the toad with receptor/tissue selective targeting. Natural selection of amphibian skin venom peptides for antipredator defence, through inter-species delivery by an exogenous secretory mode, produces subtle structural stabilisation modifications that can potentially provide new insights for the design of selectively targeted peptide therapeutics.
Amphibian,
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