本文利用Genebank中已知SARS冠状病毒基因组信息，通过Beacon Designer2软件进行分子信标探针设计，共设计出9条分子信标探针和相对应的引物，以便于为目前SARS冠状病毒快速诊断提供参考。

以茂原链轮丝菌（Streptomyces mobaraensis）的基因组为模板，利用PCR的方法扩增出谷氨酰胺转胺酶（Transglutaminase，TGase）的完整基因片段。序列分析结果表明，该片段全长1246bp，编码一个完整的ORF，长度395aa，分子量45KD。该基因的核酸序列及推导的氨基酸序列同已知微生物来源的若干TGase相比，序列相似性均很高，同酶催化活性至关重要的一些motif，如酰胺化位点等，在该氨基酸序列中也都存在；在此基础上，又进一步对其二级结构进行了分析并完成了TGase三维结构的建模。将该基因片段插入到原核表达载体pQE30xa，转化大肠杆菌E.coli JM109， SDS-PAGE结果表明，经IPTG诱导后该基因获得了表达，且表达产物有较高的活性。

对接种AM菌根真菌的大青杨苗木根际微区进行系统研究，发现AM菌根真菌对宿主根际微域环境产生了重要影响。试验中选用的Glomus mosseae、G. intraradices、G. sinuosa、G. versiforme等4种AM菌根真菌都与盆栽大青杨苗木形成了菌根复合体。其中G. mosseae、G. intraradices侵染效果最好，侵染率分别是64.4%、67.4%。受两种菌侵染的苗木的生物量分别是对照处理的2.59、2.13倍。AM菌根对根际土壤微生物种群数量没有产生影响，但使根面、根系上的细菌、放线菌、固氮菌的数量显著增加。AM菌根增加了根际土壤磷酸酶、脲酶、蛋白酶的活性，各种酶活性增加量与苗木菌根侵染率呈显著相关。AM菌根使根际pH值降低，与菌根侵染率呈显著负相关。接种苗木的根际土壤中，可直接被植物吸收利用的N、P元素出现富集现象，与菌根侵染率呈极显著相关。

The relationship between vegetation and hydrological processes is still a critical issue in ecology and environment science, especially at the landscape scale. Mingjiang valley plays an important role in water and soil resources conservation and erosion control in the upper Yangtze River. In this paper, the influence of vegetation type on hydrological processes at the landscape scale was studied using remote sensing and spatial analysis in Mingjiang valley and its five catchments. First, the vegetation distribution was mapped with high accuracy using three scenes of Landsat thematic mapper (TM) imagery and the optimal iterative unsupervised classification method. Then the spatial precipitation and actual evapotranspiration (AET) database was developed by converting the point-based data of meteorological stations to spatial surface with spatial interpolation. Cross-tabulation spatial analysis was employed to study the relationship between vegetation and rainfall, evaporation, and runoff. The results show that dominant vegetation types are grasslands, forests, and shrublands in the Mingjiang valley, with the proportions of 37.44%, 29.97%, and 22.62%, respectively. The annual precipitation ranges from 560 to 720mm in areas of conifer and mixed forests, shrublands, and grasslands. For broadleaf forests, croplands, and other vegetation types, the precipitation distribution ranges from 480 to 800mm, indicating a broader variation than that for the dominant vegetation type. In high-precipitation regions of the valley, forest vegetation covers the largest area. The precipitation is positively correlated with vegetation cover. We found that AET has a nonlinear relationship with vegetation cover, but this relationship is complicated. Our results demonstrated that the relative evapotranspiration rate (ER) is negatively correlated with precipitation, and water remaining (WR) is positively correlated with precipitation in the landscape. From the hydrological records in the Mingjiang valley, the annual mean runoff is 502 m3•s–1, the mean annual runoff amount is 140 × 109 m3, and the annual runoff rate is 0.0213 m3•s–1•km–2. We found that percent forest cover is positively correlated with percent runoff. This supports the results of previous nonspatial investigation in the valley. From scale analysis, we found that most spatial patterns of climate and hydrological variations are scale dependent, e.g., precipitation, AET, ER, WR, and runoff vary at different levels of landscape scales.

The zonation of Scirpus mariqueter and Scirpus tabernaemontani communities is conspicuous in salt marshes of the Changjiang estuary. However, diking, which partly restricted tidal fluctuation in a salt marsh, resulted in the invasion of S. tabernaemontani into S. mariqueter communities. To explore the role of the interaction between the two species in the vegetation change, the structure of S. mariqueter tussocks at low elevation and S. tabernaemontani clones at medium elevation were investigated in the diked marsh, and biomass allocations to different components of the two species was also estimated. Scirpus mariqueter performed better at the edges of the tussocks but aged and declined in the central areas of the tussocks with low density of living shoots, large proportion of flowering shoots, and low aboveground dry mass. Scirpus taberaemontani grew vigorously in the center areas of large S. taberaemontani clones with high density of shoots, and those clones were surrounded by a residual ring of S. mariqueter, whose structure was similar to the pattern observed in the tussocks of S. mariqueter. Comparison of the vegetation structure along the elevational gradient of the diked marsh suggests that S. taberaemontani invades the center area of the Scirpus mariqueter tussocks, possibly due to changed topography and its well-developed aerenchymatous tissue. Scirpus tabernaemontani allocates a significantly greater proportion of biomass to photosynthetic shoots and rhizomes than S. mariqueter does, both above ground and below ground, a potential competitive advantage. Once established in the central areas of the S. mariqueter tussocks, S. taberaemontani successfully replaced S. mariqueter. We hypothesize that the competitive displacement of S. mariqueter by S. taberaemontani plays an important role in the vegetation change after the short-term tidal restriction and also in generating plant zonation in the salt marshes.