Crop-to-wild introgression may play an important role in evolution of wild species. Asian cultivated rice (Oryza sativa L.) is of a particular concern because of its cross-compatibility with the wild ancestor, O. rufipogon Griff. The distribution of cultivated rice and O. rufipogon populations is extensively sympatric, particularly in Asia where many wild populations are surrounded by rice fields. Consequently, gene flow from cultivated rice may have a potential to alter genetic composition of wild rice populations in close proximity. In this study, we estimated introgression of cultivated rice with O. rufipogon based on analyses of 139 rice varieties (86 indica and 53 japonica ecotypes) and 336 wild individuals from 11 O. rufipogon populations in China. DNA fingerprinting based on 17 selected rice simple sequence repeat (SSR) primer pairs was adopted to measure allelic frequencies in rice varieties and O. rufipogon samples, and to estimate genetic associations between wild and cultivated rice through cluster analysis. We detected consanguinity of cultivated rice in O. rufipogon populations according to the admixture model of the STRUCTURE program. The analyses showedz that four wild rice populations, DXP1, DX-P2, GZ-P2, and HL-P, contained some rare alleles that were commonly found in the rice varieties examined. In addition, the four wild rice populations that scattered among the rice varieties in the cluster analysis showed a closer affinity to the cultivars than the other wild populations. This finding supports the contention of substantial gene flow from crop to wild species when these species occur close to each other. The introgressive populations had slightly higher genetic diversity than those that were isolated from rice. Crop-to-wild introgression may have accumulative impacts on genetic variations in wild populations, leading to significant differentiation in wild species. Therefore, effective measure should be taken to avoid considerable introgression from cultivated rice, which may influence the effective in-situ conservation of wild rice species.

Wild rice, Oryza rufipogon, has endangered species conservation status and it is subject to in situ conservation in China. To understand the potential of the seed bank in species conservation and population restoration, this study compared the genetic diversity of O. rufipogon plants with that of its soil seed banks in two marshes. A total of 11 pairs of rice SSR primers were used and 9 were polymorphic. Allele frequencies of the seeds differed significantly from those of surface plants and varied between soil layers. Relatively more alleles and higher genetic diversity (He) were found in plant populations, relative to seed banks. The numbers of germinable seeds and the level of genetic variation in seed banks decreased with the increasing of soil depth, indicating a rapid seed loss. Genetic differentiation was detected between sites and between plant and seed populations, as well as among seeds of different soil strata. Rapid seed loss, partly dormancy loss, and nonrandom seed mortality are discussed as the possible contributors to the pattern of reduced genetic variation within seed banks, compared to plants. These could also be responsible for the considerable genetic differentiation between populations. The seed population held about 72% of the total genetic variation of O. rufipogon in each marsh, indicating the potential of seed banks for restoring population variabilities if the plant populations were lost.

Determining the genetic structure of an in situ conserved population can provide insight into the dynamics of population genetic processes associated with successful plant conservation. We used 21 microsatellite loci to analyse the genetic relationships among individuals (n=813) collected from a small Oryza rufipogon population conserved since 1993 in Hunan Province of China. The analysis revealed four distinct genetic subpopulations (FST=0.145) without geographic isolation. One subpopulation was composed of possible introgressed individuals, two subpopulations were composed of seed recruits and their descendants, and the fourth subpopulation consisted of reintroduced individuals, seed recruits and their descendants. Positive spatial genetic structures were detected by spatial autocorrelation statistics at the population (c. 63 m) and subpopulation levels (11-30 m), but the degree of autocorrelation was stronger at the population level. These results showed that prejudging the cryptic structure is important before autocorrelation analysis for the entire population. Our study suggests that population history can be a significant determinant on population structure for plant restoration projects.

Genetic variability of a perennial clonal aquatic weed Leersia hexandra was estimated by the ISSR assay. A total of 225 L. hexandra individuals collected from 10 populations in China were analyzed. The 12 used ISSR primers generated 175 bands, with an average of 14.6 per primer. The average values of percentage of polymorphic bands (PPB), effective number of alleles (Ae), and gene diversity (He) in these populations were 49.7%, 1.258, and 0.154, respectively, indicating a considerable genetic diversity in this species. Significant genetic differentiation was found among populations with the major genetic variability within populations based on Nei’s genetic diversity analysis (Gst = 0.417) and AMOVA (40.1%). Genetic variation is independent of geographical distance. This pattern of genetic variation may be associated with reproductive mode of its mixed breeding systems although mainly through vegetative propagation. © 2005 Elsevier B.V. All rights reserved.

Common wild rice (Oryza rufipogonGriff.), known as the ancestor of Asian cultivated rice (Oryza sativa L.), is the most important germplasm for rice improvement. The first male sterility gene was found in the wild rice, and introduced to the cultivated rice, which launched the fast development of the high-yielding hybrid rice. Other agronomically beneficial traits in the wild rice, such as rice tungro virus resistance, bacterial leaf blight (Xa21 gene) resistance and acid sulfate soil tolerance, have played important roles in rice breeding. China has the northernmost distribution area of wild rice possessing great genetic diversity. However, most of the populations of this species have disappeared in China over the last three decades, mainly caused by habitat loss, fragmentation and other human disturbances. Unfortunately, the decline of existing populations still continues. In the present study, we reviewed studies on genetic diversity and conservation of this wild rice in China, concentrating on population structure, pollen competition, pollen/gene flow from cultivated rice to wild rice, and ecological restoration in relation to in situ conservation. The relatively high genetic diversity of populations of O. rufipogon in China suggests that there is great value for conservation. Considerable gene flow from cultivated rice to wild rice may alter the genetic structure of natural populations of O. rufipogon and eventually lead to its genetic erosion. Pollen competition between wild and cultivated rice has caused a low rate of crop-to-wild gene flow, but it does not completely prevent gene flow from the crop. Effective isolation measures should be undertaken in the regions where in situ conservation of O. rufipogon is carried out. Reintroduction is an important alternative for the in situ conservation of wild rice species. As wild rice is an important genetic resource, both in situ and ex situ conservation strategies are needed.