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【期刊论文】In vitro plant regeneration from cotyledon explants of Swainsona salsula Taubert
郑国锠, J. Yang, Z. Hu, G.Q. Guo & G.C. Zheng
Plant Cell, Tissue and Organ Culture 66: 35-39, 2001.,-0001,():
-1年11月30日
An effective protocol has been developed for plant regeneration from cotyledon explants of Swainsona salsula Taubert (Saline swainsona), a medicinal and agronomic shrub. Adventitious shoots were obtained from 83.2% of cotyledon explants from 3-day seedlings cultured on Murashige and Skoog (MS) medium containing 2.0mgl−1 thidiazuron (TDZ), with an average of 9.3 shoots per explant. Individual elongated shoots were rooted on half strengthMS medium supplemented with 2.0mgl−1 indole-3-butyric acid (IBA), with 59.3% success. Regenerated plants with well developed shoots and roots were successfully transferred to soil, without detectable variants. Histological observation revealed that shoots developed from cotyledon explants via organogenesis, with little callus.
organogenesis,, plant regeneration,, Swainsona salsula Taubert
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【期刊论文】Direct plantlet regeneration from the tuber of Stachys sieboldii
郑国锠, Wei Li, Huan-Huan Gao, Rong Lu, Guang-Qing Guo & Guo-Chang Zheng*
Plant Cell, Tissue and Organ Culture 71: 259-262, 2002.,-0001,():
-1年11月30日
Development of an efficient in vitro plantlet regeneration system from tubers of Stachys sieboldii (Miq.), a traditional Chinese medicinal plant and vegetable, is described. Adventitious shoots with an average of 9.1 (after 4 weeks of incubation) shoots per explant were obtained from 60.4% of tuber explants cultured on Murashige and Skoog (MS) medium containing 1.0mgl−1 thidiazuron (TDZ), and after another 4 weeks of incubation on growth regulator free MS medium, 33.5 shoots per explant were achieved. Subcultures showed results that were similar to those of the initial culture. Excised shoots were rooted both on MS and on half strength MS medium with a frequency of about 95%. Excised shoots were also rooted ex vitro by directly planting them into cups containing sand. Regenerated plants with well developed shoots and roots were successfully transplanted to soil, after which they developed free of abnormalities.
adventitious shoots,, Stachys sieboldii (, Miq., ), ,, tuber
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郑国锠, YU Chun-Hong, GUO Guang-Qin, NIE Xiu-Wan (NIEH Hsiu-Wan), ZHENG Guo-Chang (CHENG Kuo-Chang)*
Acta Botanica Sinica 2004, 46 (12): 1443-1453,-0001,():
-1年11月30日
Pectinase activity was localized at the ultrastructural level in pollen mother cells of tobacco (Nicotiana tabacum L.) during meiotic prophaseⅠto elucidate its role in the biogenesis of secondary plasmodesma (sPD) and cytoplasmic channel (CC). At the leptotene stage the enzyme was mainly present in the cisternae of smooth endoplasmic reticulum (SER) and their derived vesicles, but absent in the Golgi body and Golgi vesicles. Later at the zygotene stage, when sPDs and CCs were actively formed, strong pectinase activity was observed not only in the SER cisternae and their derived vesicles but also in the cell wall, especially in the vicinity of or within both simple and branched plasmodesmata, notably along the middle lamellae, which also characterized the sites of CCs being formed. The presence of exocytotic vesicles containing reaction products suggests that pectinase shares the same excretive pathway as that used by cellulase for its delivery into the wall, i.e. in active form via smooth endoplasmic reticulum (ER) and its derived vesicles by exocytosis. In combination with cellulase, pectinase also promotes the secondary formation of plasmodesmata and CCs by specifically digesting the pectin in middle lamella.
cytochemical localization, secondary plasmodesmata, cytoplasmic channel, pectinase, pollen mother cells, tobacco
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