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郑国锠
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-1年11月30日
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郑国锠
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-1年11月30日
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【期刊论文】Relationship between tissue culture and agronomic traits of spring wheat
郑国锠
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-1年11月30日
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郑国锠
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-1年11月30日
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【期刊论文】In vitro plant regeneration from cotyledon explants of Swainsona salsula Taubert
郑国锠, J. Yang, Z. Hu, G.Q. Guo & G.C. Zheng
Plant Cell, Tissue and Organ Culture 66: 35-39, 2001.,-0001,():
-1年11月30日
An effective protocol has been developed for plant regeneration from cotyledon explants of Swainsona salsula Taubert (Saline swainsona), a medicinal and agronomic shrub. Adventitious shoots were obtained from 83.2% of cotyledon explants from 3-day seedlings cultured on Murashige and Skoog (MS) medium containing 2.0mgl−1 thidiazuron (TDZ), with an average of 9.3 shoots per explant. Individual elongated shoots were rooted on half strengthMS medium supplemented with 2.0mgl−1 indole-3-butyric acid (IBA), with 59.3% success. Regenerated plants with well developed shoots and roots were successfully transferred to soil, without detectable variants. Histological observation revealed that shoots developed from cotyledon explants via organogenesis, with little callus.
organogenesis,, plant regeneration,, Swainsona salsula Taubert
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