Jujuhoside A (JUA) is a main component of jujubogenia exrracted from the seed of Ziziphus jujuba Mill var spinosa (Bunge) Hu ex H F Chou (Ziziphus), which is widely used in Chinese traitional metiicine for the treatment of insomnia and anxiety. Previously, we reported the inhibitinry effects of JuA on hippocampal forma-tion in vivo and in vitro, the present study was carried out to ex amine the effects of JuA on glutamate (Glu)-mediated excitatory signal pathway in hippocamput. Microdialysis coupled with high-performance Liquid chromatography (HPLC) was used to monitor the changes of Glu Levels in the hippocampus induced by penicillin sodium, or a mixture of penicillin sodium and JuA. The results showed that penicillin increased the hippocampal GlU concentration (p<0.Ol) and a high dose of JuA (0.1g/L) sig-mificantly blocked penicillin-indued Glu release (P<0.05). Moreover, the effect of JuA on intracellular Ca2+ changes after the stimulation by Glu was studied in cultured hippocampal neurons with can focal laser scanning microscope (CLSM). It was found that Clu (O.5 mM) reduced an intracellular [Ca2+], increase (p<0.01), and JuA significantly inhibited the Glu-induced Ca2+. increase. The calmodulin (CAM) antatgonist trifluoperazine (TFP) showed a similar inhibitory effect as JuA. These observations suggested tbat JuA has inhibitory effects on Glu-mediated excita-tory signal pathway in hippocampus and probably acts through its anti-calmodulin action

目的:观察中药酸枣仁皂俄A对青霖素钠诱导产生的大鼠海马脑片CAI区兴奋性放电的抑制作用。方法：细胞外记录离体大鼠海马脑片CAI区锥体细胞层群体峰电位.结果:青霉素钠500，100和200Ku/L可剂量依赖地诱导海马脑片上CAI区神经元的兴奋苯巴比妥钠0.02-0.05g/L和酸枣仁皂成A0.05-0.10g/L都可以剂量依赖性地抑制这种青霉素钠诱发的兴奋反应.结论:高剂量的酸枣仁皂试A能够抑制青霉素钠诱导的海马CAI区兴奋性电位.群峰电位（PS）的个数和第一个峰电位的幅度受到的抑制较明显，而兴奋性突触后场电位的变化不大。

建立一个用于体外研究特定流动剪切力作用下白细胞和内皮细胞动态相互作用的方法。利用建立的平板流动小室系统可在体外产生特定的流动剪切力。将培养的人脐静脉内皮细胞装入平板流动小室后，以0.71dynes/cm2的流动剪切力把含有吖啶橙染色的白细胞的灌流液导入流动小室，由此产生了白细胞和内皮细胞的动态粘附过程。整个粘附过程通过OlympusIX70倒置荧光显微系统观察，同时通过CCD摄象头录像。然后用图象采集卡将录像采集为数字图象并保存。利用针对实验设计的图象处理和分析方法，对采集的数字图象进行处理和测量，可以得到粘附白细胞的个数和滚动白细胞的速度。通过研究内毒素脂多糖（LPS）对内皮细胞粘附功能的促进及地塞米松（DXM）对该刺激的抑制作用来验证。对于用内毒素脂多糖（LPS）处理的内皮细胞，固定粘附和慢速滚动的白细胞的个数比对照组分别显著增加了23.7倍和4.1倍，同时白细胞在粘附作用过程中慢速滚动和快速滚动的速度比对照组明显降低了25.6%和26。1%而对于脂多糖和地塞米松（DXM）处理过的内皮细胞，上述内毒素引起的影响被显著抑制了。该方法可以用于研究不同的化学和物理刺激对内皮细胞功能的影响机制，及用来评价各类抑制内皮细胞粘附功能的药物。

A method for the direct measurement of intracellular nitric oxide (NO) production stimulated by penicillin G (PG) in cultured hippocampal neurons with diaminoanthraquinone (DAA) using laser scanning confocal microscopy (LSCM) was developed. Intracellular DAA fluorescence could specifically represent NO production based on two facts: (1) 3-morpholinosydnonimine, a NO donor, could dose-dependently increase DAA fluorescence; and (2) haemoglobin, a NO scavenger, could inhibit the increase of DAA fluorescence. The PG dose-dependently increasd the intercellular level of glutamate (Glu, 5 min after stimulation) and the intracellular NO production (30 min throughout stimulation). The increase of NO production could be reversed by Nw-nitro-L-arginine (a NO synthase inhibitor), and also by D(-)2-amino-5-phosphonovaleric acid, a subtype of Glu receptor antagonist. These results revealed that DAA could be used to indicate real-time and kinetic intracellular NO production of hippocampal neurons with higher sensitivity, specificity and accuracy.