目的 探讨海绵体神经损伤对阴茎海绵体nNOS神经纤维的影响。方法 采用免疫组织化学技术SP法测定大鼠海绵体神经损伤后阴茎勃起组织nNOS神经纤维数量。结果 双侧损伤组术后3周，阴茎海绵体nNOS神经纤维显著减少，6个月后仍很少；单侧损伤组，术后3 周损伤侧nNOS神经纤维的改变类似于双侧损伤组，但6个月后损伤侧nNOS神经纤维明显增多，接近于对侧水平。结论 阴茎海绵体nNOS神经纤维在单侧海绵体神经损伤后可能会再生，而双侧海绵体神经损伤后则不能再生，提示在进行盆腔根治性手术时，要保留一侧海绵体神经，以维持勃起功能。

目的 研究雄激素对大鼠阴茎组织中血管内皮生长因子（VEGF）及其受体Flk-1/KDR表达的影响。方法 2个月龄SD雄性大鼠随机分成假手术组（A组），去势组（B组），去势+睾酮替代组（C组），以及5α还原酶抑制剂保列治喂饲组（D组），2周后取大鼠阴茎组织行VEGF及其受体Flk-1/KDR免疫组织化学染色和VEGFmRNA原位杂交，运用计算机图像分析系统，比较其表达强度。结果 A组和C组大鼠阴茎组织VEGF免疫组织化学染色均呈高表达灰度值（99.80±2.88和98.70±3.41），而B组与D组均呈低表达灰度值（127.80±3.93和125.30±3.76），（P<0.01）；VEGFmRNA原位杂交也显示B组（159.30±9.42）与D组（157.30±9.71）阳性着色均明显少于A组（124.60±8.37）和C组（125.30±7.80），（P<0.01）；而B组与D组之间，A组和C组之间表达灰度值差异均无显著性；VEGF受体Flk-1/KDR在各组表达差异均无显著性。结论 雄激素对大鼠阴茎组织中VEGF以及VEGFmRNA的表达均有上调作用，而对VEGF受体Flk-1/KDR的表达无影响；调节大鼠阴茎组织VEGF表达的雄激素活性成分可能主要为5α-双氢睾酮。

目的 探讨胃液对膀胱癌生长抑制、细胞凋亡及bcl-2/bax表达的影响。方法 采用细胞培养、四唑盐比色试验（MTT）、DNA提取及电泳、流式细胞仪和免疫组织化学等方法，观察胃液对膀胱癌细胞系生长抑制、细胞凋亡及bd-2/bax表达的影响。结果 高浓度胃液（19～38mmol/L）抑制膀胱癌细胞系的生长繁殖，低浓度胃液（0.594～9.500mmol/L）则促进膀胱癌细胞系的生长繁殖。胃液能诱导体外培养的膀胱癌细胞凋亡。膀胱癌细胞系BIU-87中bcl-2、bax均有表达（分别为63.5%和41.4%），经胃液、稀盐酸或阿霉素处理后，bdl-2表达下降（分别为34.8%、37.3%和29.6%），bax表达上升（分别为49.3%、47.1%和52.2%），bd-2/bax比值小于1。结论 胃液能抑制膀胱癌的生长，诱导其凋亡。

Aim: To investigate alterations of smooth muscle cells and collagen fibers in corpus cavernosum following cavernous neurectomy and its relation to the expression of transforming growth factor-β1 (TGF-β1). Methods: Ten adult male SD rats (neurectomy group) were subject to a bilateral cavernous nerve (CN) resection aseptically under an operating microscope, with 6 sham-operated rats as the control. Fifteen weeks after the operation, the penile specimens were collected and prepared for quantitative-analyzing of ratio of smooth muscle to collagen fibers in corpus cavernosum with confocal microscopy, and for detecting the expression of TGF-β1 by RT-PCR and western-blot. Results: Smooth muscle cells that show red color after fluorescent-labeling with tetramethylrhodamine isothiocyanate-phalloidin and collagen fibers that produce green autofluorescence after paraformaldehyde fixation were clearly identified under the confocal microscope. Quantification of fluorescent intensity showed that the ratio of smooth muscle to collagen fibers in corpus cavernosum in neurectomy group was 0.265±0.125, which was significantly lower than that in sham-operated group (0.760±0.196, P<0.01). RT-PCR and western-blot analyses revealed a significantly higher expression of TGF-β1 in the penile tissues of the neurectomy animals than that in sham-operated group. Conclusion: Bilateral ablation of CN can lead to fibrosis of corpus cavernosum, which may be related to an increased expression of TGF-β1 induced by hypoxia in cavernous tissue after denervation.