中国科技论文在线

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2005年01月17日

【期刊论文】A modified molecular beacon combining the properties of TaqMan probe

宓怀风， De-Ming Kong， a， Long Gu， Han-Xi Shen*a and Huai-Feng Mi ab

CHEM. COMMUN., 2002, 854-855，-0001，（）：

-1年11月30日

A modified molecular beacon that possesses a stem-hairpinstructure as seen in conventional molecular beacons and canbe cleaved during PCR is designed, and it can specificallyrecognize the presence of the target and was obviously moresensitive than conventional molecular beacons.

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2005年01月17日

【期刊论文】Simulation of TaqMan by two single-labelled probes

宓怀风， De-Ming Kong， a， Long Gu， Han-Xi Shen*a and Huai-Feng Mi ab

CHEM. COMMUN., 2002, 2584-2585，-0001，（）：

-1年11月30日

摘要

A novel method for duplex probes is designed to simulate theTaqMan probe during polymerase chain reaction (PCR); two single-labelled probes are used for this method, whichrelies on the 5'-exonuclease activity of nucleic acid polymerase.

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2005年01月17日

【期刊论文】Real-time quantitative assay of telomerase activity using the duplex scorpion primer

宓怀风， Yanping Huang，， Deming Kong， Yi Yang， Ruifang Niu， Hanxi Shen， * & HuaifengMi

Biotechnology Letters 26: 891-895, 2004.，-0001，（）：

-1年11月30日

摘要

A novel real-time quantitative method for measuring telomerase activity is described in which the duplex scorpionprimer is used to provide an intramolecular probing mechanism for specific detection of telomerase activity. Usingthis method, linearity from 10 to 104 cells expressing telomerase activity could be obtained (R2=0.994). Therequirement of post-PCR steps is thus obviated.

duplex scorpion primer,， real-time quantitative PCR,， telomerase activity,， TRAP assay

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2005年01月17日

【期刊论文】PCR hot-start using duplex primers

宓怀风， Deming Kong，， *， Hanxi Shen， Yanping Huang & HuaifengMi

Biotechnology Letters 26: 277-280, 2004.，-0001，（）：

-1年11月30日

摘要

A new technique of PCR hot-start using duplex primers has been developed which can decrease the undesirableproducts arising throughout PCR amplification thereby giving better results than a manual hot-start method.

duplex primers,， hot-start,， PCR

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2005年01月17日

【期刊论文】Duplex probes: a new approach for the detection ofspecific nucleic acids in homogenous assays

宓怀风， De-Ming Kong a， Yan-Ping Huang a， Xiao-Bin Zhang a， Wei-Hong Yang c， Han-Xi Shen a， *， Huai-Feng Mi a， b

Analytica Chimica Acta 491 (2003) 135-143，-0001，（）：

-1年11月30日

摘要

A novel method for the detection of specific nucleic acids in homogenous solution was developed. The method is based onthe use of duplex probes in which fluorescent donor and quencher labeled on either oligonucleotide are held in close proximity, so that fluorescence is quenched. Amplification of the target sequence results in the cleavage of the probe and the resultingfluorescence can be detected. The fluorescent data analysis demonstrated that the duplex probes can specifically recognize thepresence of target, and a significantly higher lever of relative fluorescent signal than TaqMan probes is obtainable. Combinedwith real-time PCR instruments, the assay can be used to quantify the input target molecules. As few as five copies of initialtarget molecules can be detected, and a large dynamic linear ranger (five orders of magnitude) is obtained.

Duplex probe， TaqMan， Detection of specific nucleic acids

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