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冯喜增, Likai Wang, Xizeng Feng, ∗ Sen Hou, Qilin Chan and Ming Qin
SURFACE AND INTERFACE ANALYSIS Surf. Interface Anal. 2006; 38: 44-50 Published online inWiley Inter Science,-0001,():
-1年11月30日
Microcontact printing (μCP) is aneasy andefficientway of producing patterns of self-assembledmonolayers (SAM) containing different functional groups. We have developed a simple and convenient μCP-based technique for the modification of a mica substrate with 3-aminopropyltriethoxysilane (APTES) and the micropatterning of proteins (chicken IgG and rabbit IgG) on the modified mica surface. Our approach provides a quick and easy way to produce protein patterns on solid surfaces. The printed immunoglobulin patterns were detected by exposing the substrate to solutions containing fluorescently labeled complementary anti-IgGs, and the formed immunoassays were studied using fluorescence microscopy. Copyright 2005 John Wiley & Sons, Ltd.
microcontactprinting(, μCP), , surfacemodification, mica, immunoassays
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冯喜增, 刘涛, 陈龙, 侯森, 薛永来, 冯喜增*
科学通报,2006,51(4):399~403,-0001,():
-1年11月30日
设计并制备了一种新的具有分子探针功能的主体分子型非病毒载体-邻菲啰啉-β-环糊精衍生物主体分子(DZY-1),应用凝胶电泳法研究探讨了DZY-1与DNA相互作用及客体分子对该主体分子诱导DNA凝聚的影响;并进一步研究探讨了单一主体分子、主/客体分子配合物诱导DNA分子凝聚和聚合所形成的超分子聚合物抗限制性内切酶(HindⅢ)酶解的特性. 应用扫描电子显微镜(SEM)观测到该主体分子、主/客体分子配合物与DNA分子相互作用形成超分子聚合物的微观结构形态。阐述了其作为非病毒基因载体可能的传递机制,为设计、制备新的非病毒基因载体提供了一种新途径。
邻菲啰啉-β-环糊精衍生物, λ-非病毒基因载体, 凝胶电泳, 扫描电子显微镜
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冯喜增, 王立凯冯喜增*
化学进展,2005,12(3):482~498,-0001,():
-1年11月30日
微流控芯片最初起源于分析化学领域,是一种采用精细加工技术,在数平方厘米的基片,制作出微通道网络结构及其它功能单元,以实现集微量样品制备、进样、反应、分离及检测于一体的快速、高效、低耗的微型分析实验装置。随着微电子及微机械制作技术的不断进步,近年来微流控芯片技术发展迅猛,并开始在化学、生命科学及医学器件等领域发挥重要作用。本文首先简单介绍了微流控芯片制作材料和工艺,然后主要阐述了其在蛋白质分离、免疫分析、NDA分析和测序、细胞培养及检测等方面的应用进展。
微流控芯片免疫分析, DNA分析, 细胞培养
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【期刊论文】Microcontact Printing Techniques in Bioscience*
冯喜增, FENG Xi-zeng*, *, HOU Sun, CHAN Qi-lin, WANG Li-kai, QIN Ming and HAN Pei-dong
CHEM, RES. CJMESE U. 2004, 20 (6), 826-832,-0001,():
-1年11月30日
The microcontact printing (CP) technology available for patterning protein, DAN hybridixation, immu-nossay and cellures onto solid surface are reviewed. This review describes some of the techniques currently employed for creating two-dimensional biomolcular microarry, and the research results regarding also presented.
Microcontaceprinting(, CP), ,, DNAhybridization,, Immunoassy,, Celladhesion
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【期刊论文】Organic Synthesis in Micro Reactors
冯喜增, XiZeng Feng, Stephen J. Haswell and Paul Watts*
Current Topics in Medicinal Chemistry 2004, 4, 707-727,-0001,():
-1年11月30日
This article reviews the current and future applications of micro reactors in the field of chemistry, biochemistry and drug discovery. The fabrication and physical characterisation of micro reactors, together with details of their use and operation is described. Liquid and gas phase reactions have been used to illustrate the advantages of performing chemical reactions in micro reactors. A brief evaluation of the possible advantages that micro fabrication could offer in developing biological applications, based on miniaturized devices is also presented.
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