在不同操作电压和温度下，SDS-蛋白质在无胶筛分电泳的实验结果表明，电泳力学迁移模型（EMMM）比传统经验式更能准确地反映实验事实。SDS-蛋白质的相对构象参数（Rc）随热力学温度增加而变化，在288～298K范围内Rc迅速变小，而在298～313K范围内，温度变化对其影响不大。不同的SDS-蛋白质在一定条件下具有相近的活化能，该结论进一步从热力学方面支持了EMMM的一个基本结论，即不同相对分子质量Mr的SDS-蛋白质在同-NGS实验条件下具有相似的形状或构象，并与SDS-蛋白质分子为长棒状这一客观事实相符合。

将电泳力学迁移模型（EMMM）推广到了毛细管无胶筛分电泳（NGS）体系，即具有相等电荷密度的溶质，其电泳速率的对数与其相对分子质量的对数成正比。DNA在全筛分剂质量分数范围（0～2.0%）内的NGS实验结果表明，EMMM与传统经验式比较，以前者为优。随着筛分剂质量分数的增加，DNA片段的相对构象参数Rc有规律地增加，这主要是由于筛分剂网孔的大小和形状变化引起的，而DNA本身的构象并不发生变化。

首次将电流变（ER）学引入毛细管电泳(CE)，提出了电致固化筛分剂的概念，探讨了ER流体在CE中的潜在应用。结果表明，ER流体由于其独特的电-粘-剪切、电学特性和空间网状结构，可形成电致固化筛分的新型CE分离模式。

把电泳力学迁移模型（EMMM）扩展到了毛细管筛分电泳,并采用多组文献实验数据验证了EMMM。进一步阐明了EMMM中构象参数Rc的物理含义，并确定了Rc的取值范围。通过与文献经验关系式相比，EMMM能够更精确地符合实验事实。

The evolution of solute zone under gradient elution is a typical problem of non-linear continuity equation since the local diffusion coefficient and local migration velocity of the mass cells of solute zones are the functions of position and time due to space- and time-variable mobile phase composition. In this paper, based on the mesoscopic approaches (Lagrangian description, the continuity theory and the local equilibrium assumption), the evolution of solute zones in space- and time-dependent fields is described by the iterative addition of local probability density of the mass cells of solute zones. Furthermore, on macroscopic levels, the recursion equations have been proposed to simulate zone migration and spreading in reversed-phase high-performance liquid chromatography (RP-HPLC) through directly relating local retention factor and local diffusion coefficient to local mobile phase concentration. This new approach differs entirely from the traditional theories on plate concept with Eulerian description, since band width recursion equation is actually the accumulation of local diffusion coefficients of solute zones to discrete-time slices. Recursion equations and literature equations were used in dealing with same experimental data in RP-HPLC, and the comparison results show that the recursion equations can accurately predict band width under gradient elution.