目的检测及克隆小鼠B淋巴瘤A20细胞株mCD99L2基因，构建真核表达载体。方法设计寡核苷酸探针，应用原位分子杂交方法检测A20细胞中mCD99L2基因的mRNA表达，RT-PCR法从A20细胞总RNA中获取mCD99L2基因含编码区全长cDNA．克隆AT载体，筛选阳性克隆、酶切鉴定并经序列测定；设计含酶切位点的PCR引物，PCR获取含mCD99L2基因编码区片段，用限制性内切酶EcoR I和Xhol双酶切取所需目的片段，利用分子克隆技术与pcDNA3.1/MycHis C(+)质粒重组，对产生的重组子进行PCR双酶切鉴定，并经过测序证实。结果原位杂交方法检测A20细胞中mCD99L2基因mRNA呈阳性表达；RT-PCR法成功获得mCD99L2基因编码区全长cDNA序列，DNA序列测序结呆与GenBank提供的已知序列(NM_138309)比较，结果完全一致；重组质粒pcDNA3.1-mCD99L2中的插入片段经DNA测序后与GenBank中mD99L2基因相应序列比较．100%同源。结论小鼠B淋巴瘤A20细胞株存在mCD99L2基因，采用RT-PCR和T-A技术克隆了A20细胞的mCD99L2基因，成功构建了pcDNA3.1-mCD99L2真核表达载体，为下一步探索mCD99L2基因在小鼠B淋巴瘤A20细胞株中的功能奠定了实验基础。

目的 通过检测口腔鳞癌及癌前病变中抑癌基因与张力蛋白同源10 q丢失的磷酸酶基因( PTEN)、3、4、5-三磷酸磷脂酰肌醇(PIP3)、细胞周期蛋白D1(cyclin D1)的表达，分析其相关性，初步探讨在口腔鳞癌中PTEN可能的作用途径。方法采用免疫组化SP法检测63例口腔鳞癌、29例单纯增生、33例异常增生、25例正常口腔黏膜中PTEN、PIP3、cyclin D1的表达。结果PTEN在口腔鳞癌中阴性和弱阳性表达占25%，明显低于其他各组；PIP3在单纯增生、异常增生和口腔鳞癌中阳性或强阳性表达分别占66%、64%和76%，明显高于正常组的表达；cyclin D1在口腔鳞癌中阳性或强阳性表达的占49%，明显高于其他各组。PTEN与PIP3、cyclin D1之间呈负相关，PIP3写cyclin D1呈正相关。结论PTEN在口腔鳞癌的发生和发展过程中起着一定的作用；在口腔鳞癌中PTEN可通过下调PIP3，继而下调cyclin D1，从而抑制细胞的生长。

目的：检测mic2基因与CD99蛋白和Eber-1基因与潜伏膜蛋白-1（LMP1）在经典型霍奇金淋巴瘤(cHL) H/RS细胞中的表达，探讨mic2/CD99表达与Eber-1/LMP-1的相关性。方法：采用分子原位杂交和免疫组化技术并结合组织芯片技术检测59例石蜡包埋淋巴瘤组织标本[43例cHL，16例非霍奇金淋巴瘤(NHL)] mic2/CD99和Eber-1/LMP-1的表达，比较分析mic2/CD99在两组中的表达及与Eber-1/LMP -1的关系。结果：cHL组CD99蛋白表达阳性率为2.3%，mic2基因表达为55.8%，LMP1袁达为58.1%，Eber-1表达为53.5%；NHL组CD99蛋白与mic2基因表达高于ChL组(P<0.05)；LMP1和Eber -1的表达低于Chl组(P<0.05)；mic2基因的表达高于CD99蛋白的表达(P<0.05)；Eber -1与LMP1的表达无统计学意义(P>0.05)。CD99蛋白与LMP1的表达呈负相关(P<0.05)，各项指标的表达与性别无关。CD99蛋白与LMP1的表达与年龄呈负相关(P<0.05)，mic2与Eber -l的表达与年龄无关(P>0.05)。结论：CD99蛋白在从cHL H/RS细胞中低表达，并与LMP1的表达呈负相关。

AIM: To examine the expressions and distribution of Caspase-3, TNF-α and TNFRI and their relationship to apoptosis in oral lichen planus (OLP). METHODS: Immunohistochemical staining with SP method and TUNEL technique were used to detect the expressions of Caspase-3, TNF-α and TNFRI in 33 cases of OLP and 7 cases of normal oral mucosa specimens (the controls). RESULTS: Compared with the controls, OLP was increased in apoptosis index (AI) of epithelial cells and decreased in AI of lymphocytes (both P<0.05). The expression of Caspase-3 in all OLP cases was significantly higher than that in the normal group both in the epithelial layer and in the lamina propria (P<0.025). Compared with that in the normal group, TNF-α expression in all OLP cases was decreased in the epithelial layer and increased in the lamina propria (both P<0.025), while TNFRI expression was increased in the epithelial layer and decreased in the lamina propria in all OLP cases(both P <0.025). TNF-α expression was significantly increased in the lamina propria in the erosion group compared with the non-erosion group (P<0.05). A positive correlation was observed between Caspase-3 expression and AI in the epithelial cells(r=0.631, P<0.05). However, there was no significant correlation between Caspase-3 expression and AI in lymphocytes (P >0.05). Expressions of TNF-α and TNFR1 were significantly correlated to AI both in the epithelial layer and in the lamina propria. CONCLUSION: The accelerated apoptosis of keratinocytes and the inhibition of lymphocyte apoptosis may coexist in OLP, which may contribute to the form ation and progression of OLP.

目的：探讨 Caspase-3和 FIEN蛋白在口腔扁平苔藓病变发生发展中的作用及两者间的关系。方法：采用 S-P免疫组化方法对 28例口腔扁平苔藓及 5例正常口腔黏膜中 caspase-3 和 FIEN蛋白表达进行检测。结果：口腔扁平苔藓病损中 Caspase-3的阳性率上皮层和固有层均为100％，与正常口腔黏膜相比，表达有显著统计学意义(P<0.01)。口腔扁平苔藓病损中FI'EN蛋白阳性率为100％，与正常口腔黏膜相比，无统计学差异(P>0.05)。口腔扁平苔藓中caspae-3与 FI'EN蛋白表达呈正相关，(P<0.05)。结论：Caspase-3和 FI'EN蛋白均参与口腔扁平苔藓病变的发生及发展过程，且两者之间具有相关性。