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【期刊论文】DNA Fragments of Three Parental Strains Existing in GEMs Fhhh
程树培, HAO Chun-bo, YAN Jun, QU Meng-meng, Wang Dong, CHENG Shu-pei*, GU Ji-dong, QIU Wan-fei, WANG Yin-yin
,-0001,():
-1年11月30日
There were 6 target DNA fragments of the three parental strains existing in the cell of genetically engineered microorganism strain (GEMs) Fhhh measured in this research by polymerase chain reaction (PCR). And Fhhh was a fusant GEMs of the three parental strains Phanerochaete chrysosporium; Saccharomyces cerevisiae and the native bacterium YZ1, and they have the 6 target DNA fragments of mnp1, mnp2、lip1、lip2,FLO1 and 16SrDNA respectively. The results showed that Fhhh contained the total 6 target DNA fragments with the molecular genetic stability. Meanwhile the PCR productions of the three parental strains only had their target DNA fragments individually and were different from each other. It may illustrate that the technique of the inter-kingdom protoplast fusion through the process of intercellular gene recombination can be used as a reliable bioengineering technique to construct the specific stain for the pollution control.
mnp1, mnp2, lip1, lip2, FLO1, 16SrDNA, PCR
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【期刊论文】Predication of Fhhh Potential in PTA Wastewater Treatment
程树培, SHI Lei, Cheng Shu-pei*, Yan Jun, Zhang Xu-xiang, Ding Zhong-hai. Hao Chun-bo
,-0001,():
-1年11月30日
Ebis is the intelligent environmental biotechnological informatics software developed for judging the effectiveness of the microorganism strain in the industrial wastewater treatment system (IWTS) at the optimal status. The parameter, as the objective function for the judgment, is the minimum reactor volume (Vmin) calculated by Ebis. The rationality and the universality of Ebis were demonstrated in the domestic sewage treatment system (DSTS) with the data published in USA and China. Fhhh strain's potential for treating the purified terephthalic acid (PTA) has been proved in this research. It suggests that Ebis would be useful and universal for predicating the technique effectiveness in both DSTS and IWTS.
software, strain, potential, predication, the purified terephthalic acid wastewater
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【期刊论文】Pilot regulation of MnP-SA for treating PTA wastewater
程树培, SUN Shi-lei, CHENG Shu-pei*, WAN Yu-qiu, ZHANG Xu-xiang, SHI Lei, ZHU Cheng-jun, YU Hong-xia, LUO Xiang, LU Jian-hua, ZHANG Xiao-chun, WANG Gui-lin, WANG Hong-li, YU Jing-zhou, Chen Jun
,-0001,():
-1年11月30日
In the pilot study of treating the purified terephthalic acid (PTA) wastewater with the functional strain Fhhh in the carrier activated sludge process (CASP), the ratio of COD: TN: TP and the concentrations of Cu, Mn, Se and Zn were controlled to improve the manganese peroxidase (MnP) levels for increasing the treatment efficiency. When the ratio of COD:TN:TP was 100: 0.36: 0.15 and the concentrations of Cu, Mn, Se and Zn were 0.54, 5.07, 0.00 and 0.08mg/L, the MnP specific activity (MnP-SA) reached 689 U/L, and the sludge loading rate to COD (SLRC) was 1.09 d-1, which was 4-7fold of that in other processes reported. The data indicates that improving MnP level could enhance the degradability of Fhhh. And the potentials of Fhhh and CASP will be also discussed in this paper.
functional strain, PTA wastewater, pilot engineering, MnP-SA, regulation, SLRC
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程树培, J. Yan, S.P. Cheng. X.X. Zhang. L. Shi. C.J. Zhu
Bull Environ Contain Toxicoi (2004) 72: 387-393,-0001,():
-1年11月30日
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程树培, Ji-Guang Gu, , Shu-Pei Cheng, Jianhua Liu, and Ji-Dong Gu
Journal of Polymers and the Environment, Vol. 8, No.4, October 2000 (q2002),-0001,():
-1年11月30日
An electrochemical impedance spectroscopy (EIS) technique was evaluated for monitoring microbial degradation of electronic packaging polyimides. The microbial inoculum was a mixed culture of fungi isolated previously from deteriorated polyimides. The active fungal consortium comprised Aspergillus versicolor, Cladosporium cladosporioides, and a Chaetomium species. After inoculation, fungal growth on the polyimides resulted in distinctive EIS spectra indicative of polymer insulation failure, which directly related to polymer integrity. Degradation appeared to occur in a number of steps and two distinctive stages in the decline of film resistance were observed in the inoculated EIS cells within the 2 and 10 weeks after inoculation. The early stage of resistance decrease may be related to the ingress of water molecules and ionic species into the polymeric materials, whereas the second stage probably resulted from partial degradation of the polymers by fungal growth on the polymer film. The relationship between changes of impedance spectra and microbial degradation of the polymer was further supported by scanning electron microscopy (SEM) observations of fungi growing on the surface of the inoculated polyimides. Our data indicate that the EIS can be used in detection of early degradation of resistant polymers and polyimides that are susceptible to biodeterioration.
Polyimides, electronic insulation, biodegradation, biodeterioration, fungi, electrochemical impedance spectroscopy.,
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