To find unknown protein-coding genes, annotation pipelines use a combination of ab initio gene prediction and similarity to experimentally confirmed genes or proteins. Here, we show that although the ab initio predictions have an intrinsically high false-positive rate, they also have a consistently low false-negative rate. The incorporation of similarity information is meant to reduce the false-positive rate, but in doing so it increases the false-negative rate. The crucial variable is gene size (including introns)-genes of the most extreme sizes, especially very large genes, are most likely to be incorrectly predicted.

Few studies on the irradiation effects on dehydration characteristics and quality of dried-product ha ve been reported in the past three decades. This study focuses on drying characteristics of bio-products by hot-air after they are pretreated by gamma irradiation. Experiments were made to study the influence of different irradiation dose, air temperature, slice thickness of apple on the ehydration rate, and appearance quality (L-values), vitamin C content, and the rehydration ratio of dried apple. A conclusion is reached that the three factors of irradiation dose, hot-air temperature, and initial slice thickness significantly affected the four indices of appearance quality (L-values), vitamin C content, dehydration rate and rehydration ratio.

Foods are frequently subjected to dynamic loading during harvesting, handling, transportation, and processing. Accurate prediction of dynamic responses of foods under these loading conditions requires better understanding of their dynamic visco-elastic properties. In this article, dynamic equilibrium was determined according to sinusoidal stress-strain. When a visco-elastic specimen is subjected to a sinusoidal varying stress, the resulting strain will be a sinusoidal response of the same frequency as the stress, out of phase by a lag angle. Experiments were conducted to measure the complex modulus of intact Daguanshan peaches and Luyu pears, the phase angle by which the strain lags the stress, and their relationship with initial pre-loading and vibration input power over a range of frequencies. Both the complex modulus and the phase angle increased as the frequency increased from 20 to 200Hz. Initial pre-loading had a significant effect on the complex modulus, but not on the phase angle. Vibration input power had no significant effect on the complex modulus and phase angle. The phase angle depends not only on internal friction but also on the inertia of intact fruit.