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2004年12月29日

【期刊论文】The cytological mechanism of low fertility in the naked seed rice

石春海， J.G. Wu， C.H. Shi， S.Y. Chen & J.F. Xiao

Genetica 121: 259-267, 2004.，-0001，（）：

-1年11月30日

The low fertility of naked seed rice (NSR) was investigated by the following observations: somatic chromosome constitute, behavior of pollen mother cells (PMCs), the germination of mature pollen grains, the development of male and female gametes and the structure of the anther opening. The results indicated that somatic chromosomal number was 2n=24, behavior of PMCs were normal and most of pollen grains could regularly develop further to mature male gametophytes in NSR. And dehiscence chamber and thickened endothecium cell (TEC) in numerous anthers of the NSR were developed abnormally after dicaryotic phase, result in few anthers complete opening and most partly opening or failure to opening, therefore much fewer of pollen grains attach on the stigma as compared with normal variety. Furthermore most of embryo sacs possessed abnormal structure and were sterile. All of above illustrated that the failure of the anther opening and the abortion of female gametophyte were main factors controlling the low seed-setting rate of the NSR.

cytological mechanism,， female gametophyte,， fertility,， male gametophyte,， naked seed rice,， Oryza sativa

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2004年12月29日

【期刊论文】Developmental Behavior of Gene Expression for Brown Rice Thickness Under Different Environments

石春海， C.H. Shi， * J.G. Wu， and P. Wu

genesis 33: 185-190(2002)，-0001，（）：

-1年11月30日

摘要

The dynamic changes of genetic effects, including main effects, and genotype

rice， Oryza sativa L.，， filling stages， brown rice thickness， unconditional genetic variance， conditional genetic variance

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2004年12月29日

【期刊论文】Prediction of grain weight, brown rice weight and amylose content in single rice grains using near-infrared reflectance spectroscopy

石春海， J.G. Wu*， C.H. Shi

Field Crops Research 87(2004)13-21，-0001，（）：

-1年11月30日

摘要

The potential of near-infrared reflectance spectroscopy (NIRS) for simultaneous analysis of grain weight (mg), brown rice weight (mg) and milled rice amylose content (AC, %) in single rice grains was studied. Calibration equations were developed using 474 single grain samples, scanned as both rice grain and brown rice. An independent set containing 90 F2 generation grains was used to validate the equations. In general, equations developed using the first derivative resulted in superior calibration and validation statistics compared with the second derivative and those developed using brown rice were superior to those developed from the rice grain. Fitting equations were developed and monitored with an external validation set. The standard error of prediction (corrected for bias) SEP(C) for AC, brown rice weight and rice grain weight for equations developed using brown rice were 2.82, 1.09 and 1.30, with corresponding coefficient of determinations (r2) of 0.85, 0.71 and 0.67, and SEP(C)/S.D. of 0.39, 0.57 and 0.59, respectively. It was demonstrated that NIRS provides a convenient way to screen single intact grains. This will be advantageous in early generation selection in rice breeding programs.

Rice， Single grain， Rice grain weight， Brown rice weight， Amylose content (， AC)，， Near-infrared reflectance spectroscopy (， NIRS)，