ese sulfated derivates were evaluated by activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT) and platelet aggregation assay. The results showed that YCP sulfates significantly prolonged APTT, TT and PT. The derivates showed no effects on thrombin in the presence or in the absence of antithrombin Ⅲ (AT Ⅲ) or heparin cofactor II (HC Ⅱ), while the derivates effectively inhibited factor Xa in the presence of AT Ⅲ. At the same time, YCP-SH also possessed potent antiplatelet aggregation activity in vitro compared with aspirin. YCP sulfates specifically interfered with different stages of the coagulation cascade, and the anticoagulant activity improved with the increasing DS and decreased Mw.

为了实现用微生物发酵法生产辅酶Q10，筛选出一株辅酶Q10高产菌株，并对其从C源、N源、最适温度、最适pH值、通氧量、添加前体等方面进行了优化。筛选出的菌株用不同的碳源、氮源在37℃培养24h，检测其CoQ10含量；以富马酸为碳源、玉米浆为氮源、35℃、pH7.2发酵24h辅酶Q10含量达87mg/L；通氧量对该菌生长及辅酶Q10合成有促进作用；添加前体对羟基苯甲酸、异戊二烯、β2胡萝卜素均可有效提高辅酶Q10产量，其中异戊二烯可提高产率178.4%。该菌辅酶Q10产量较高，经TLC2UV法定量，HPLC法进行纯度检测，红外、质谱分析进行成品鉴定，其纯度达99.5%。

The reactive oxygen species (ROS) and free radical-initiated reactions are ascertained to play multiple roles in degenerative or pathological events such as aging, cancer, heart dysfunction and Alzheimer's disease. EPS2 with a mean molecular weight of 1.3×105 was characterized as an antioxidant exopolysaccharide from the broth of a marine filamentous fungus Keissleriella sp. YS 4.08. Compositionally, it is composed of galactose, glucose, rhamnose, mannose and glucuronic acid in an approximate proportion of 50∶8∶1∶1∶0.4. The radical eliminating and antioxidant actions of the glycan was assessed in different in vitro systems showing that EPS2 exhibited profound scavenging activities in superoxide radical. As a reinforcement of the action, similar radical scavenging effects of EPS2 were also discerned with both site-specific and non site-specific hydroxyl radical using the deoxyribose assay method. Moreover, EPS2 effectively blocked as well the non site-specific strandbreaking of DNA induced by the Fenton reaction at concentrations of 0.1 and 1mg/mL. Further investigation of the effect of EPS2 on human low density lipoprotein (LDL) system demonstrated that it significantly inhibited copper-mediated oxidation of LDL in a dose-dependent manner. These results suggest that EPS2, possessing pronounced free radical scavenging and antioxidant activities, could be of considerable preventive and therapeutic significance to some life-threatening health problems such as cancer, atherogenesis and Alzheimer's disease which pathologically initiated by the presence of free radicals leading to the inevitable peroxidation of important biomolecules.

目的建立凋亡素基因克隆及原核表达的方法。方法根据已报道的鸡贫血病毒凋亡素基因设计合成一对引物，通过PCR扩增，构建重组质粒pUC182VP3，将其与pET30质粒分别用限制性内切酶NdeⅠ、EcoRⅠ消化，构建原核表达载体pET302VP3，用以转化感受态的EcoliDE3，经IPTG化学诱导表达，表达产物进行SDS2PAGE电泳鉴定。结果凋亡素基因在EcoliDE3中获得了大量表达。结论该结果对凋亡素进一步开发为抗肿瘤新药具有重要意义。

D-Nerve growth factor (13 NGF) mRNA levels in hippocampa from CsvBL/6J mice of different ages were compared by semi-quantitative RT-PCR method, taking 13-actin gene as an internal control. The levels of 6-and 12-month-old C57 mice were much lower compared with that of the 1-month-old mouse. However, there was no significant difference between these two groups. This result suggests that gene ranscription level of hippocampus 13 NGF in a C57 mouse descends evidently as it grows up. Base T at Site 294 of 13 NGF cDNA was substituted by C in the C57 mouse, as revealed by DNA sequencing for the first time. Nevertheless, this polymorphism of nucleotide did not make any difference in amino acid composition.