Although peroxisome proliferator-activated receptor (PPAR) is widely expressed in many tissues, the role of PPAR is poorly understood. In this study, we report that PPAR was up-regulated in vascular smooth muscle cells (VSMC) during vascular lesion formation. By using Northern blot analysis, we demonstrated that PPAR was increased by 3-4-fold in VSMC treated with platelet-derived growth factor (PDGF) (20ng/ml). In addition, PDGF-induced PPAR mRNA expression neither needs de novo protein synthesis nor affects the stability of PPAR mRNA in VSMC. Preincubation of VSMC with phosphatidylinositol 3-kinase inhibitor (LY294002, 50mol/liter) or infection of VSMC with an adenovirus carrying the gene for a dominant negative form of Akt abrogated PDGF-induced PPAR mRNA expression, suggesting that phosphatidylinositol 3-kinase/Akt signaling pathway is involved in the regulation of PDGFinduced PPAR mRNA expression in VSMC. To explore the role of PPAR in VSMC, we generated rat vascular smooth muscle cells (A7r5) stably overexpressing PPAR and the control green fluorescent protein. Overexpression of PPAR in VSMC increased post-confluent cell proliferation by increasing the cyclin A and CDK2 as well as decreasing p57kip2. Taken together, the results suggest that PPAR plays an important role in the pathology of diseases associated with VSMC proliferation, such as primary atherosclerosis and restenosis.

目的：通过观察IFN-alb对肝星状细胞（HSC）形态和表达CIGF的影响，探讨IFN-alb在治疗纤维化中的作用机制。方法：采用体外培养大鼠肝星状细胞，分别加入IFN-alb、TGF-β1，观察肝星状细胞的形态学及CTGF表达的改变。结果：透射电镜观察到IFN-alb作用组肝星状细胞有明显的凋亡现象发生；RT-PCR观察到正常组和1GF-β1诱导肝星状细胞表达的CTGF mRNA随IFN-alb的作用而减少。结论：IFN-alb对肝星状细胞表达CT-GF的抑制作用机制可能是通过诱导肝星状细胞凋亡和抑制TGF-β1诱导肝星状细胞表达CTGF。

目的 观察人间质胶原酶（MMPl）基因高效表达对体外肝纤维化的影响。方法 利用DNA重组技术构建了pcDNA3-MMPl真核表达重组质粒。经脂质体包裹后，转染张氏传代人肝细胞，Westem blot检测MMPl蛋白表达量。在体外乙醇诱导纤维化培养条件下，抗生蛋白链菌素一生物索复合物标志（ISAB）免疫组织化学法检测肝细胞周围Ⅰ、Ⅲ型胶原生成状况，图象分析仪半定量分析胶原纤维的相对含量（RCC）。结果 成功构建真核表达质粒pcDNA3-MMPl，检测Rcc显示未转染组为132.6±5.7，转染组为118.8±4.8；统计学分析表明未转染组明显高于转染组（P<O.01）。结论 本研究证明重组质粒pcDNA3-MMPl能在体外致纤维化培养条件下拮抗肝细胞间质过多胶原产生。

目的 评价改良全肝血流阻断法在肝切除术中的安全性及应用价值。方法 回归分析近年11例常规方法不能切除的中央型肝肿瘤病例，采用全肝血流阻断法，2例同时利用静脉一静脉转流方法，1例利用体外循环静脉转流技术。结果 1例术中不能耐受全肝血流阻断术中死亡，其余10例顺利切除肿瘤，手术时间327.3±117.8min，3例术后出现右胸积液，1例心房纤颤。结论 全肝血流阻断方法对减少术中出血无优势，且术后并发症发生率高，宜仅选择性应用于肿瘤侵犯肝静脉主干、腔静脉或伴有腔静脉癌栓者。