Objective. This study aimed to determine the growth inhibitory effects of transfecting the wild-type p16 gene into human salivary adenoid cystic carcinoma (SACC) cells in vitro. Methods. p16 cDNA was cloned into pDOR-neo plasmid by recombination technique, and the human eukaryotic expression vector, pDOR-p16, was constructed. P16 gene was transfected into SACC83 cells using the liposome method, and the expression of the transfected genes was detected by RT-PCR. The efficacy of transfection was determined by MTT-based colorimetric formats. The DNA metabolic rate of transfected cells was measured by 3H-TdR incorporation. To describe the cell division phase, the cell cycles of the p16-transfected gene cells and untransfected cells were analyzed by flow cytometry. Results. The growths in vitro of the p16-transfected cells were inhibited significantly. 3H-TdR incorporation showed a decreased DNA metabolic rate of p16-transfected cells, and flow cytometry suggested a significant increase of the cells in the G1 phase and decrease in the S phase.

目的 研究神经生长因子（NGF）、神经节苷脂M1（GM1）及NGF和GM1混合液对桥接面神经的硅胶管腔中面神经管状化移植再生的影响。方法选择成年大白兔40只，4只作正常对照，其余36只随机分为A、B、C三组，将其双侧面神经下颊支与腮腺前缘相同平面造成8mm长缺损，用1.5mm的硅胶管进行桥接，然后作以下处理：A组，左侧硅胶管内加入NGF，右侧加入生理盐水；B组，左侧加入NGF+GM1，右侧加入NGF；C组，左侧加入NGF+GM1，右侧加入GM1。于术后20周进行电生理及组织学观察。结果A组左右侧神经传导速度差异无显著性；B、C两组左右侧间差异有显著性（P<0.05）。且两组左侧再生神经有髓纤维数目、直径、髓鞘厚度、轴突直径均高于对侧，差异有显著性（P<0.05），但A组以上指标均无差异。光镜及电镜观察试验各组均有较明显的神经再生。结论 ①NGF能够促进运动神经元再生，但这种能力有限；⑦GM1能够介导NGF促进运动神经元再生，表现出良好的生物学效应。

目的 基质金属蛋白白酶-2与口腔鳞癌颈淋巴结转移的关系。方法 通过蛋白酶谱岳对口腔鳞癌（OSCC）癌组织、正常口腔粘膜组炽厦血浆中基质金属蛋白酶-2（MMP-2）含量进行了研究。并与口腔鳞癌的临床病理特征进行统计学分析。结果 口腔鳞痞OSCC癌组织、正常口腔粘膜厦血浆中均存在MMP-2，而OSCC癌组织厦血裴中MMP 2含量升高与OSCC的颈淋巴结转移有关；OSCC癌组炽中MMP-2含量高于正常口腔粘膜组织。结论 MMP-2可作为判断口腔鳞癌颈淋巴结转移的指标。