Matrix metalloproteinases (MMPs) and their tissue inhibitors play important roles in the remodeling of extracellular matrix (ECM). MMP-26, also called endometase or matrilysin-2, is a novel member of the MMP family. The present study was to investigate the temporal and spatial expression of MMP-26 mRNA in mouse uterus during the estrous cycle and early pregnancy by using in situ hybridization and semi-quantitative RT-PCR. In this study, MMP-26 mRNA was found to be localized to the luminal and glandular epithelium at proestrus and estrus,and the expression level was decreased significantly from metestrus to dioestrus. During pre-implantation period, MMP-26 mRNA was predominantly expressed in luminal and glandular epithelium at much higher level;whereas it switched to stroma during peri-implantation period, and also appeared in the blastocysts and the implantation sites. The results suggested that MMP-26 might play a role in the cycling changes of mouse uterus during the estrous cycle and embryo implantation.

stroma surrounding the implanting blastocyst, and Smad4 mRNA were accumulated in the decidua near the luminal epithelium. Both Smads were present in the decidua on days 6-7 with a switch from the mesometrial pole to the antimesometrial pole. RT–PCR results showed that both Smad2 and Smad4 mRNA levels were rising during peri-implantatation. The results suggest that Smad2 and Smad4 might be involved in the cycling changes of mouse uterus during the oestrous cycle and embryo implantation.

目的研究肿瘤坏死因子TNF-对正常精子顶体酶活性和顶体反应的影响及其机制方法采用BAEE/ADH联合法测定顶体酶和三色染色法技术测定顶体反应结果TNF-可显著降低精子顶体酶的活性和顶体反应P0.01 P0.01并且它可使精子中的Ca2+-ATPase和SOD的活性显著降低P0.05 P0.001但TNF-可使精子中NOS活性增强及NO含量增加P0.001 P0.001对Na+-K+-ATPase活性影响不明显P0.05结论TNF-对精子顶体酶及顶体反应有一定的抑制作用并且可能通过降低Ca+-ATPase活性自由基和NO及增加NOS等多种途径来实现的.

黄体由卵巢排卵后的卵泡所形成，为一临时性内分泌器官，主要功能是产生孕酮和雌激素。无论是月经黄体还是妊娠黄体都要经历形成、维持与溶解的过程，该过程与血管新生、稳定和退化密切相关。血管内皮生长因子（vascularendothelialgrowthfactor，VEGF）是近年被确认在调节新血管形成中起重要作用的因子，它能特异性地刺激血管内皮的增生和迁移，并能增强血管渗透性，稳定成熟血管内皮。目前的研究表明，VEGF在血管新生及黄体的形成和功能中发挥重要作用。研究VEGF与黄体形成和功能的芙系可以为临床某些疾病如早期流产、过期妊娠、卵巢黄体功能障碍所致的不孕症等病因探讨提供有意义的理论依据。

目的研究雌、孕激素对子宫内膜癌HH+I细胞端粒酶活性及细胞周期的影响。方法用：LIN$F6，OI法和实时荧光定量N*L技术检测雌、孕激素作用前后HH+I子宫内膜腺癌细胞系端粒酶活性及：FLL2I的表达，流式细胞仪检测细胞周期及细胞凋亡的变化。结果雌激素对HH+I细胞端粒酶活性和>：FL：的表达有明显增强作用，而孕激素对其影响不显著。孕激素作用下，7期细胞比例明显增高，O期及R期细胞比例明显降低：细胞的分裂、增殖受到明显抑制：凋亡细胞比例明显增加。雌激素的作用则相反。结论雌激素能增强子宫内膜癌细胞端粒酶活性和的表达，促进其分裂和增殖，降低凋亡细胞比例，孕激素的作用相反。