The expression and regulation of metalloproteinases-2,-9(MMP-2,-9) and their tissue inhibitors TIMP-1,-2,-3mRNA were studied in this experiment. In the PMSGhCG primed pseudopregnant rat, MMP-2,-9mRNA levels were the highest at Day 1, decreased from Day 4, and reached the minimal level at Day 8, then increased at Day 14; no significant changes were observed in TIMP-2 mRNA expression from Day 1 to Day 14; TIMP-3 mRNA expression was the lowest at Day 1, increased from Day 4, reached the maximal level at Day 8, and persisted to Day 14. TNF-a could significantly increase the expression of MMP-2,-9 and TIMP-1 mRNA in the in vitro perfused pseudopregnant CL, and decrease the expression of TIMP-3 mRNA, but had no effect on TIMP-2 mRNA expression. The results indicate that MMP-2,-9and TIMP-1,-2,-3 might be involved in the regulation of CL function and maintenance of CL structure via their coordinated gene expression. TNF-a could inhibit luteal regression via increasing MMP-2,-9andTIMP-1 mRNA in the in vitro perfused pseudopregnant ovary.

α肿瘤坏死因子转换酶（tumor necrosis factor-αconverting enzyme，TACE）是ADAM蛋白质家族的成员之一，又称ADAM17。TACE是锚定于细胞膜的细胞表面的蛋白质，是一种多结构域的Ⅰ型跨膜蛋白，具有显著的金属蛋白酶水解活性，对TNF-α前体有水解作用。TACE 的胞内区富含脯氨酸，并包含一个潜在的磷酸化位点KKLDKQYESL，该位点具有和SH3区域相结合的潜能；很可能具有信号转导的功能，或对亚细胞定位有重要作用。

AIM; To investigate the location alteration of Smad2 and Smad4 mRNAs in the liver during and after fibrogenesis in rats. METHODS; Eighty male Wistar rats weighing approximately 200g each were used. The rat models of experimental hepatic fibrosis were established by injection with carbon tetrachloride(CCl4), normal rats and rats were injected with olive oil and served as control groups. In situ hybridization(ISH)was used to detect the Smad2 and Smad4 mRNA in liver. RESULTS; In situ hybridization showed Smad2 and Smad4 mRNA expressions in the cytoplasm of hepatic stellate cells(HSC), fibroblasts and myofibroblasts around the central vein and hepatic sinus during and after fibrogenesis. Expression of Smad2, 4 mRNA was higher than that in normal and control rats. CONCLUSION; In the process of and after hepatic fibrosis formation, HSC, fibroblasts and myofibroblasts are the major cells that express Smad2 and Smad4. The more serious the hepatic fibrosis is in the injured liver, the higher the level of Smad2 and Smad4 gene expression is during and after fibrogenesis respectively.