The distributions of PNA binding glycoconjugates in the plasma membrane of Acrida cinerea Thunberg germ cells were detected using biotin labeled PNA, for better understanding of the formation and changes of glycoconjugates during oogenesis. The ultrastructure of vitellogenesis also was observed by electron microscopy for detection of the origin and track of vitelline material. In the ovary, PNA receptors appeared in the oocyte cytoplasm of the second phases of oo-genesis; positive granules gradually increased from the third phase to the fourth, and they exhibited a maximum expres-sion before the vitellogennic stage in the cytoplasm of the oocyte. From the vitellogennic to chorionation stage, positive granules gradually declined. Binding sites on follicle cells were changed with their morphological variation in every stage of oogenesis. The vitelline of A 1cinerea formed within the oocyte by degrees. The results suggest that PNA receptors and yolk materials are synthesized by the oocyte at an early period. With the development of the oocyte, some exogeous mate-rials from two sources act as PNA receptors and others take part in vitelline synthesis. One is blood lymph that offers some useful materials to the oocyte directly through follicle cell gaps; the other are follicle cells that produce and transmit some materials to oocyte to support vitellogenesis. In addition, PNA receptors secreted by follicle cells participate in the forma-tion of yolk membrane [Acta Zoologica Sinica 51 (5): 932-939, 2005].

利用还原型烟酰胺腺嘌呤二核苷酸（NADPH）黄递酶组织化学方法，对瘦露螽Phaneroptera gracilis Burmeister 配子发生中一氧化氮合酶（nitric oxide synthase, NOS）分布进行了定位研究。结果表明，一氧化氮合酶阳性反应发生在瘦露螽精子发生中的各级生精细胞的胞质中，成熟精子呈阴性。各级未成熟卵母细胞胞质均呈一氧化氮合酶阳性反应，胞质着色为深蓝黑色，核区不明显。随着卵黄颗粒的逐渐形成，胞质中的一氧化氮合酶阳性产物逐渐减少，直到卵黄颗粒完全形成。卵泡细胞在卵黄颗粒形成之前呈一氧化氮合酶阴性反应，在卵黄颗粒完成后，卵泡细胞的胞质中开始呈一氧化氮合酶阳性反应，直至卵壳的形成。提示一氧化氮参与了瘦露螽配子发生。

Jun氨基末端激酶（Jun N-terminal kinase, JNK）是一种重要的细胞信号传递者。它参与了细胞生长、分化、程序性死亡等生理过程，而且在调节上皮细胞运动和形态发生等方面也起着重要作用。大量研究证实，在果蝇Drosophila的背闭合行为（dorsal closure, DC）中，DJNK（Drosophila JNK）的调节是关键。文章就果蝇DC的发生过程以及DJNK信号途径的研究进展作一简要的综述。

通过对吉林省四平地区6个蝗虫优势种绿牧草蝗Omocestua viridulus （L.），黄胫小车蝗Oedaleus infernalis Sauss，素色异爪蝗Euchorthippus unicolor （Ikonn.），条纹异爪蝗E. vittatus Zheng，异翅负蝗Atractomorpha heteroptera B.-Bienko和长翅素木蝗Shirakacris shirakii （I.Bol.）的野外养殖，从成虫出现到死亡对6个蝗虫优势种卵巢季节动态变化进行了观察和测量研究。结果表明，（1）不同发育历期成虫体长变化差异不显著，而卵巢形态和体积变化差异显著；（2）卵巢发育具有明显的种间差异；（3）卵巢发育速度呈现先慢后快的增长模式；（4）6个蝗虫优势种卵巢发育符合5个发育级别的划分标准。

为研究昆虫卵泡刺激素（FSH）和黄体生成素（LH）的存在与分布，我们用免疫细胞化学染色技术对FSH和LH在尖唇散白蚁（Reticulitermes aculabialis）脑中的分布进行了定位研究。结果显示：FSH免疫阳性神经细胞及神经纤维分布在雌性繁殖蚁分飞期前脑的左叶、右叶及中部，雌性末龄若虫前脑的右叶，雄性繁殖蚁前脑的右叶；LH免疫阳性物质分布在雌、雄繁殖蚁前脑左叶；在工蚁和兵蚁中未见有FSH和LH免疫阳性物质存在。研究结果表明：FSH和LH在白蚁脑中的存在具有发育阶段特异性，并且有可能像其在脊椎动物中那样参与调节性腺发育及精卵成熟；可能调节繁殖蚁、兵蚁、工蚁的产生[动物学报50（2）：240-244，2004]。