The influence of traditional Chinese medicine (TCM) formulation from Astragalus Root (Radix astragalin seu Hedysari) and Chinese Angelica Root (R. Angelicae Sinensis) at a ratio of 5:1 (w/w) on non-specific immunity of Jian carp, Cyprinus carpio var. Jian was investigated. The number of NBT-positive cells in the blood and lysozyme and complement activities in the serum of Carp fed with commercial feed supplemented with 1.0% (diet 1) and 1.5% (diet 2) TCM at 10 day of post-feeding were not different from those of the control group fed with feed unsupplemented TCM 10 days post-feeding (P>0.05), but at 20 and 30 days they increased significantly (P<0.05). The values of diet 1 group and diet 2 group at 20 day and at 30 day were not significantly different (P>0.05) from each other. In addition, the TCM formula increased body weight of experimental fish by about 16.84% (diet 1) and 19% (diet 2) above that of the control group. Therefore, these data suggest that the TCM formula could elevate the function of non-specific immunity of Jian carp. The optimal dosage added to commercial carp feed was 1.0% (w/w) and the oral administration time as a course of treatment was 20 days.

The influence of traditional Chinese medicine (TCM) formulated from Astragalus Root (Radix astragalin seu Hedysari) and Chinese Angelica Root (Radix angelicae sinensis) at the rate of 5:1 (w/w) on nonspecific immunity including the number of phagocytes, lysozyme and complement activities, and disease resistance to Vibrio alginolyticus in large yellow croaker, Pseudosciaena crocea (Richardson) was determined. Minced fish (surimi) supplemented with 0.5% TCM had no influence on the nonspecific immunity of the fish (P>0.05). On the other hand, the surimi with 1.0% and 1.5% TCM resulted in a significant increase in nitroblue tetrazolium (NBT) positive cells and lysozyme activities (P<0.05) of experimented fish on 20, 25 and 30 days after feeding and led to evident enhancement of complement activities (P<0.05) after 15 days of feeding. The highest survival rates (93.3%) in fish infected with V. alginolyticus were achieved with surimi containing 1.0% and 1.5% TCM, but the cumulative mortalities were 37% and 75% in fish fed with surimi containing 0.5% TCM and TCM-free surimi, respectively. These results indicated that the TCM formula might elevate the function of nonspecific immunity and enhance disease resistance of large yellow croaker.

从海水网箱养殖的患病军曹鱼(Rachycentron canadum)的肾脏、肝脏等实质器官及腹水中分离出1株致病菌即JA一46茵。其特点如下：革兰氏阴性短杆菌，极端单鞭毛，大小为(1～2)μm×(0.5～1)μm，氧化酶和过氧化氢酶阳性，有运动性，最适温度28～30℃，需盐生长，未见发光，对弧茵抑制剂0/129敏感(150mg/L)，发酵葡萄糖不产气，在TCBS上茵落呈黄色。经API-20E 系统试荆盒检测和ATB 自动鉴定系统鉴定，该茵为创伤弧茵(Vibrio vulnificus)；其半数致死量(LD50)为4.1×10个细茵。药敏试验结果表明，氯霉素、复方新诺明、庆大霉素、环丙沙星、红霉素、氟哌酸、先锋霉素和丁胺卡那霉素等8种药物对该菌株有抑制作用，但该菌株对青霉素和氨苄青霉素不敏感。