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邢婉丽, Guangxin Xiang a, b, c, Liangbin Pan a, Lihua Huange, Zhongyao Yu e, Xindong Songa, Jing Chenga, d, Wanli Xing a, ∗, Yuxiang Zhoua
Biosensors and Bioelectronics 22(2007)2478-2484,-0001,():
-1年11月30日
Microelectrode arrays (MEAs) provide a means to investigate the electrophysiological behavior of neuronal systems through the measurements from neuronal culture preparations. Changes in activity patterns of neuronal networks are usually detected by applying neural chemicals. Because of the difficulties of fabricating the arrays, and the delicate and less reliable properties of cortical neurons, MEA-based systems with cortical neuronal networks for neurophamacological applications are technically difficult, therefore restricting their utility. Here, we report a new approach to the development of such MEA-based system with sensitive and durable MEAs conveniently fabricated and the culture conditions optimized. Upon growth differentiation, cortical neurons, cultured directly on MEAs, reach a developmentally stable and reliable activity state. With this system, we monitored the global spontaneous activities of neuronal networks and demonstrated the fine discrimination for specific substances and unique property of cortical neurons, which validated both the applicability and necessity of such system in pharmacological bioassay.
Microelectrode array, Neuropharmacology, Cortical neurons, Microfabrication, Electrophysiology, Spontaneous activity
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【期刊论文】Portable fiber-optic immunosensor for detection of methsulfuron methyl
邢婉丽, Wan-Li Xing a, *, Li-Ren Ma b, Zhong-Hua Jiang b, Fu-Huan Cao a, Ming-Hong Jia c
Talanta 52(2000)879-883,-0001,():
-1年11月30日
This fiber optic sensor, based on competitive immunoreactions between coating-haptens and free haptens in solutions with corresponding antibodies, was developed to determine the concentration of the free hapten, methsulfuron-methyl. The ovalbumin (OVA)-methsulfuron-methyl conjugate was immobilized on a microscope slide. Horseradish peroxidase (HRP) labeled goat anti-rabbit IgG was used to generate an optical signal. The portable optical device consisted of a 0.25-W tungsten-halogen light source and a photosensitive diode detector. A typical competitive-binding calibration curve was seen between 0.3 and 100 ng:ml of methsulfuron-methyl. The detection limit for methsulfuron-methyl was 0.1 ng/ml.
Fiber-optic biosensor, Immunosensor, Methsulfuron-methyl, Pesticide detection
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