中国科技论文在线

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2010年07月01日

【期刊论文】The Self-catalytic Esterification Reaction of O-Phosphoryl Serine Derivative

陈忠周， Jin Tang DU， Yan Mei LI∗， Zhong Zhou CHEN， Shi Zhong LUO， Yu Fen ZHAO

Chinese Chemical Letters Vol.16, No.7, pp 856-858, 2005，-0001，（）：

-1年11月30日

O-Phosphoryl serine derivative can perform self-catalytic esterification reaction in the mixture of CH3OH and CHCl3 at the room temperature. The phosphoryl group participation was the key step of the esterification. This type of reactions were proposed through an intermediate of mixed phosphoric-carboxylic anhydride that might provide a clue to the function of the phosphoryl group in the phosphorylated enzymes and in the prebiotic synthesis of protein.

O-Phosphoryl serine， self esterification， phosphoryl group participation.，

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2010年07月01日

【期刊论文】磷酰化氨基酸反应性质的理论研究

陈忠周，杜进堂，李艳梅，谭波，江洋，钟儒刚，赵玉芬

高等学校化学学报，2005，26，540～545，-0001，（）：

-1年11月30日

摘要

采用量子化学计算研究了磷酰化氨基酸的反应性质, 包括N-磷酰氨基酸成肽反应、成酯反应、磷上酯交换反应和磷上的N→O 迁移反应的机理以及计算模型和计算方法的选择, 并从理论上解释了自然界选择α-氨基酸而不是β, γ-氨基酸的实验事实.

N-磷酰氨基酸，五配位磷中间体，量子化学计算，从头算，密度泛函

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2010年07月01日

【期刊论文】A common intermediate for prebiotic synthesis of proteins and nucleosides: a density functional theory (DFT) study on the formation of penta-coordinate phosphorus carboxylic-phosphoric mixed anhydride from N-phosphoryl amino acids

陈忠周， Bo Tan a， *， Matthew C. Lee b， Meng Cui c， Tong Liu c， Zhong-Zhou Chen a， Yan-Mei Li a， Yong Jua， Yu-Fen Zhao a， Kaixian Chen c， Hualiang Jiang c

Journal of Molecular Structure (Theochem) 672 (2004) 51-60，-0001，（）：

-1年11月30日

摘要

N-phosphoryl amino acids (PAA) are a unique chemical species with many chemical reactivities and properties, including the ability to self-assemble into oligopeptides. Because of their chemical characteristics, they have been proposed as the common origin for both nucleic acids and proteins. In this paper, we discussed six intramolecular mixed carboxylic-phosphoric anhydrides (IMCPAs) as possible intermediates for the prebiotic synthesis of biopolymers from PAA. We also investigated the role of water molecules in the formation of IMCPA using density functional theory B3LYP quantum mechanical calculations at the 6-31G** and 6-311 G** levels. Our results showed that the energy barrier of IMCPA formation was reduced from 19.5 kcal mol21 to 9.9 kcal mol21 when water participation and solvent effects were considered. Thus, the direct participation of one water molecule and the bulk solvent effects both play important roles in the formation of IMCPA.

Origins of life， N-phosphoryl amino acids， Intramolecular mixed carboxylic-phosphoric anhydride

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2010年07月01日

【期刊论文】酶催化脱保护方法及在生物有机化学中的应用

陈忠周，李艳梅，赵刚，赵玉芬

有机化学，2001，21（4）：273-277，-0001，（）：

-1年11月30日

摘要

系统地介绍和评述了近年来氨基、羧基、巯基和羟基等基团的酶催化脱保护方法的进展。由于酶催化脱保护方法具有反应条件温和（中性，水溶液）、选择性和专一性高、副反应少等优点，故在多肽缀合物、糖等敏感多官能团化合物的研究中有很广阔的应用前景。

酶催化，脱保护，氨基，羧基，羟基，多肽缀合物

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2010年07月01日

【期刊论文】Hydrolysis of Dipeptide Heptyl Esters with Newlase F

陈忠周， Zhong Zhou CHEN， Yan Mei LI*， Xiu Fen KOU， Xue LI， Fu Rong HUANG， Hai Yan WANG， Ming Ming MA， Yu Fen ZHAO

Chinese Chemical Letters Vol.12, No.8, pp 693-696, 2001，-0001，（）：

-1年11月30日

摘要

Newlase F is a rude enzyme which contains triacylglycerol lipase and acid protease. Hydrolysis of dipeptide heptyl esters with Newlase F was studied in phosphate buffer-organic solvent by HPLC. When the Newlase F's level reached 5 mg/mL under mild condition (pH 7.0, 30℃), the lipase had the highest activity. The reaction was also affected greatly by organic solvents and their concentrations. It is found that protease in Newlase F does not hydrolyze amide bond under this condition (pH 7.0, r.t.).

Newlase F， dipeptide heptyl esters， HPLC.，

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