Respiratory infections, including Mycoplasma pneumoniae (Mp), contribute to asthma pathobiology. To date, the mechanisms underlying the increased susceptibility of asthmatics to airway Mp infection remain unclear. Short palate, lung, and nasal epithelium clone 1 (SPLUNC1) protein is a recently described large airway epithelial cell-derived molecule that was predicted to exert host defense activities. However, SPLUNC1 function and regulation in an infectious or allergic milieu are still unknown. We determined host defense and anti-inflammatory functions of SPLUNC1 protein in Mp infection and the regulation of SPLUNC1 by Mp and allergic inflammation (e.g., IL-13). SPLUNC1 function was examined in Mp or human airway epithelial cell cultures by using SPLUNC1 recombinant protein, overexpression and RNA interference. Human and mouse bronchial epithelial SPLUNC1 was examined using immunostaining, Western blotting, ELISA, laser capture microdissection, and real-time PCR. Mouse models of Mp infection and allergic inflammation and air-liquid interface cultures of normal human primary bronchial epithelial cells were used to study SPLUNC1 regulation by Mp and IL-13. We found that: 1) SPLUNC1 protein decreased Mp levels and inhibited epithelial IL-8 production induced by Mp-derived lipoproteins; 2) normal human and mouse large airway epithelial cells expressed high levels of SPLUNC1; and 3) although Mp infection increased SPLUNC1, IL-13 significantly decreased SPLUNC1 expression and Mp clearance. Our results suggest that SPLUNC1 serves as a novel host defense protein against Mp and that an allergic setting markedly reduces SPLUNC1 expression, which may in part contribute to the persistent nature of bacterial infections in allergic airways.

The biomimic reactions of N phosphoryl amino acids are very important in the study of many biochemical processes, it was found that the α-COOH group, and not the γ-COOH, was involved in the ester exchange on phosphorus experimentally. The reactions, had been proposed to be related to intramolecular penta coordinate phosphoric carboxylic mixed anhydiides. N Dimethylphosphoryl glutamic acid (DMP Glu) was selected as model compound to study the reactivity difference between the α-COOH and γ-COOH group. From MNDO calculations, the energy of the penta coordinate phosphoric intermediates contain ing five membered ring from a COOH was 40.8 ld/mol lower than that of the seven membered one from γ-COOH. This resnlt was in agreement with those from HF/6-31G** and B3LYP/6 31G** calculations. Theoretical tln-ee dimensional potential energy snrface of frothing the intermdiates predicted that the transition states 4 and 5 involving α-COOH or γ-COOH gronp had energy baniers of △E=196.1 and 216.9 kJ/mol, respectively. So the α-COOH conld be differentiated from γ-COOH intramolecnlarly in glutantic acid by N phosphorylation.

O-Phosphoryl serine derivative can perform self-catalytic esterification reaction in the mixture of CH3OH and CHCl3 at the room temperature. The phosphoryl group participation was the key step of the esterification. This type of reactions were proposed through an intermediate of mixed phosphoric-carboxylic anhydride that might provide a clue to the function of the phosphoryl group in the phosphorylated enzymes and in the prebiotic synthesis of protein.

系统地介绍和评述了近年来氨基、羧基、巯基和羟基等基团的酶催化脱保护方法的进展。由于酶催化脱保护方法具有反应条件温和（中性，水溶液）、选择性和专一性高、副反应少等优点，故在多肽缀合物、糖等敏感多官能团化合物的研究中有很广阔的应用前景。

Newlase F is a rude enzyme which contains triacylglycerol lipase and acid protease. Hydrolysis of dipeptide heptyl esters with Newlase F was studied in phosphate buffer-organic solvent by HPLC. When the Newlase F's level reached 5 mg/mL under mild condition (pH 7.0, 30℃), the lipase had the highest activity. The reaction was also affected greatly by organic solvents and their concentrations. It is found that protease in Newlase F does not hydrolyze amide bond under this condition (pH 7.0, r.t.).