已为您找到该学者13条结果 成果回收站
梁子才, Hong-Yan Zhang, , Jianping Mao, Daixing Zhou, Yunhe Xu, HaÊkan Thonberg, Zicai Liang, * and Claes Wahlestedt
Nucleic Acids Research, 2003, Vol. 31, No.14 e72,-0001,():
-1年11月30日
A solution-based method, mRNA accessible site tagging (MAST), has been developed to map the accessible sites of any given mRNA in high throughput fashion. mRNA molecules were immobilized and hybridized to randomized oligonucleotide libraries. Oligonucleotides speci®cally hybridized to the mRNA were sequenced and found to be able to precisely define the accessible sites of the mRNA. A number of ways were used to validate the accessible sites defined by the MAST process. Mapping of rabbit b-globin mRNA demonstrates the efficacy and advantage of MAST over other technologies in identifying accessible sites. Antisense oligonucleotides designed according to the accessible site map of human RhoA and Renilla luciferase mRNA result in knockdown effects that are in good correlation with the degrees of accessibility. The MAST methodology can be applied to mRNA of any length using a universal protocol.
-
64浏览
-
0点赞
-
0收藏
-
0分享
-
60下载
-
0
-
引用
【期刊论文】Chemical nanoprinting: a novel method for fabricating DNA microchips
梁子才, Anil Kumar and Zicai Liang*
Nucleic Acids Research, 2001, Vol. 29, No.2 e2,-0001,():
-1年11月30日
We have developed a novel cost-effective procedure, namely 'chemical nanoprinting', for oligonucleotide or cDNA chips manufacture. In this thermo-controlled process, the oligonucleotides, covalently attached to a highly loaded 'master-chip' through disulfide bonds, are chemically transferred to the acrylamide layer mounted on a 'print-chip'. It is demonstrated here that multiple identical print-chips can be produced from a single master-chip. This duplication process is a few hundreds of times faster than any existing methods and the speed of process and cost incurred are independent of the scale of the DNA chips.
-
11浏览
-
0点赞
-
0收藏
-
0分享
-
39下载
-
0
-
引用
【期刊论文】Silanized nucleic acids: a general platform for DNA immobilization
梁子才, Anil Kumar, Ola Larsson, Daniela Parodi and Zicai Liang*
Nucleic Acids Research, 2000, Vol. 28, No.14,-0001,():
-1年11月30日
We have developed a method for simultaneous deposition and covalent cross-linking of oligonucleotide or PCR products on unmodified glass surfaces. By covalently conjugating an active silyl moiety onto oligonucleotides or cDNA in solutions we have generated a new class of modified nucleic acids, namely silanized nucleic acids. Such silanized molecules can be immobilized instantly onto glass surfaces after manual or automated deposition. This method provides a simple and rapid, yet very efficient, solution to the immobilization of prefabricated oligonucleotides and DNA for chip production.
-
19浏览
-
0点赞
-
0收藏
-
0分享
-
39下载
-
0
-
引用