利用荧光光谱与电化学循环伏安法研究了盐酸普鲁卡因与DNA相互作用。在盐酸普鲁卡因与DNA的荧光光谱滴定实验中，随着DNA浓度的增加，盐普的荧光强度明显减小，但峰位置没有发生移动；同时，循环伏安的研究结果表明，随着DNA浓度的增加，盐普的氧化峰电流也逐渐降低，且峰电位负移，表明盐普与DNA生了较强的相互作用。

The probe effect on the apparent image of biological atomic force microscopy was explored in this study, and the potential of AFM in conformational study of gene related biological processes was illustrated by the specific nanostructural information of a new antitumor drug binding to DNA.

The binding behavior of several fluorescence dyes to calf thymus DNA has been studied by absorption, fluorescence and atomic force microscopy (AFM), which could provide direct evidence of formation modes and the corresponding nanostructural features of the ligand-DNA complexes.

A series of new dansylamide derivatives have been synthesized and the specific binding affinity of such fluorophores to nucleic acids has been investigated by using absorption, circular dichroism (CD), fluorescence and atomic force microscopy (AFM). The results indicate that the positive charge of the ligand and the π-π stacking between the dansyl part of the ligand and the DNA base pair may play an important role when binding to polynucleotides.

Dansylamide? has been modified by the introduction of several side chains into the sulfonamide substituent. Compounds with no, or with only one positive charge at the side chains were found not to be useful for association studies with nucleotides or nucleic acids. Relatively high binding constants with moderate base selectivities have been observed with a ligand obtained from dansyl chloride and N,N9-bis(3-aminopropyl)piperazine, which contains two dansyl units and two permanently charged peralkylammonium centers. Interactions of this ligand with double-stranded nucleic acids show a biphasic binding, the first at base pair concentrations below 1027 M being only detectable by a decrease of fluorescence intensity. The second phase is characterized by increased fluorescence and by wavelength changes similar to effects observed in lipophilic solvents and by affinities of up to 5×105 M. At higher concentrations bathochromic shifts and extinction changes in UV spectra of the dye suggest an intercalation mechanism, in line with preliminary circular dichroism studies.