In order to compare the adjuvant activity of compound Chinese herbal medicinal ingredients (cCHMIs) and filtrate best prescription anddose, two initial screened cCHMIs were prepared and mixed with Newcastle disease (ND) vaccine respectively at three doses to vaccinate17-day-old chickens, taking the non-adjuvant and oil adjuvant vaccines as controls. On day 7, 14, 21, 28, 35 and 42 after vaccination, thedynamic changes of peripheral lymphocyte proliferation and serum antibody titers in the chickens were determined by means of MTT methodand hemagglutination inhibition test. The results showed that two cCHMIs could promote peripheral lymphocyte proliferation and enhanceserum antibody titer, of which adjuvant activity was similar to and even stronger than oil adjuvant in promoting lymphocyte proliferation atsome time points, and there was a certain dose-effect relationship. The best adjuvant activity of cCHMIs 2 in promoting cellular immunityoccurred at middle dose, and cCHMIs 1 in enhancing humoral immunity, at high dose. Based on good synergistic effects of their components,two cCHMIs would be expected as new-type immunologic adjuvants to substitute for oil adjuvant.

The purpose of these experiments is to study the effects of Chinese herbal medicinal ingredients (CHMIs) on peripherallymphocyte proliferation and serum antibody titer in chicken vaccinated with Newcastle disease (ND) vaccine. Nine CHMIswere chosen for the experiments. Astragalus polysaccharide (APS), Isatis root polysaccharide (IRPS), Epimedium flavone(EF), Propolis flavone (PF), Astragalosides (AS) and Ginsenosides (GS) could promote lymphocyte proliferation andantibody titer, while Epimedium polysaccharide (EPS) mainly stimulated cellular immune responses. Chinese angelicapolysaccharide (CAPS) and Propolis polysaccharide (PPS) exerted weaker effects on promoting immune responses. APS,IRPS, PPS and PF in promoting lymphocyte proliferation, and IRPS, PPS, EF and PF in promoting humoral immunity inhigher dose were significantly stronger than in lower dose. Our results indicated that almost all of the nine CHMIs couldpromote both humoral and cellular immune responses and would be expected as the component drug of a new-typeimmunopotentiator.

将安全浓度范围内的黄芪多糖（ast ragalus po lysaccharide,A PS） 和淫羊藿多糖（epimedium po lysaccharide, EPS）分别加入到培养12 h 和24 h 的鸡胚成纤维细胞（CEF） 中，测定加药后CEF 增殖率和成层率的动态变化。结果表明,2 种多糖均能促进细胞增殖和延缓细胞衰老，EPS 的作用强于APS，具有一定的量效、时效关系。

分别将5种浓度的黄芪多糖（APS）、当归多糖（CAPS）、淫羊藿多糖（EPs）、板蓝根多糖（IRPS）、蜂胶多糖（PPS）、黄芪黄酮（AF）、淫羊藿黄酮（EF）、蜂胶黄酮（PF）、黄芪皂甙（AS）和人参皂甙（GS）10种中药成分加入到培养24 h的鸡胚成纤维细胞（CEF）中，再培养12 h后接种新城疫病毒（NDV），于病毒接种后72h用中性红染料吸收法测定CEF活性以评价各中药成分对细胞增殖及其抵抗病毒感染的影响。结果表明，10种中药成分均能不同程度地促进细胞增殖和抵抗病毒感染，前者以AF、APs、IRPs、PF和Gs的作用较强，后者以Gs和IRPs的作用较强，部分中药成分在两方面作用中各有所长，且有一定的量效关系。

将5种浓度的当归多糖、黄芪多糖、板蓝根多糖、淫羊藿多糖、蜂胶多糖、淫羊藿黄酮、蜂胶黄酮、黄芪皂苷和人参皂苷分别与新城疫病毒La sota株混合感作144h和272h后，加到已培养36h的鸡胚成纤维细胞（CEF）中，继续培养72h，采用中性红染料吸收法测定CEF增殖变化，判定中药成分对病毒感染活性的影响。结果表明，所有中药成分几乎所有浓度均影响或显著抑制病毒的感染活性，且与感作时司有一定的相关性。