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曲显俊, Fu-Jun Gaoa, , Shu-Xiang Cuib, Ming-Hui Chena, Yan-Na Chenga, Li-Rui Suna, S.G. Wardc, Norihiro Kokudod, Wei Tanga, d, Xian-Jun Qua
Life Sciences 83(2008)815-820,-0001,():
-1年11月30日
Aims: Des-γ-carboxyl prothrombin (DCP) is a serum protein produced by hepatocellular carcinoma (HCC) cells. The aim of this study was to evaluate the angiogenic activity of DCP in HCC cells. Main methods: The proliferation of HCC cells was measured by 3-[4, 5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. The growth of HCC cells was also evaluated in vivo by using the xenografts in nude mice. The enzyme-linked immunosorbent assay (ELISA) was employed to measure the levels of angiogenic factors in supernatant of cell culture. The expression of angiogenic factors was examined by Western blot analysis and immunohistochemical staining. Key findings: DCP displayed the stimulation of HCC cell growth in a dose (5–80 ng/ml) and time (24–96 h) dependent manner. The increase of cell growth was also observed in nude mice bearing well-established, palpable HepG2 and SMMC-7721 xenografts after 2 weeks administration of DCP. HCC cell growth was accompanied by the elevated levels of angiogenic factors. The levels of vascular endothelial growth factor (VEGF), transforming growth factor-alpha (TGF-α) and basic fibroblast growth factor (bFGF) in supernatant of SMMC-7721 cells were increased from 47, 126, and 60 pg/106 cells/24 h to 400, 208, and 298 pg/106 cells/ 24 h, respectively, after 72 h incubation with 80 ng/ml of DCP. The results of Western blot analysis and immunohistochemical staining of HCC xenografts also showed the significant increase of VEGF, TGF-α, and bFGF in HCC cells.
Des-gamma-carboxy prothrombin (, DCP), Hepatocellular carcinoma cells (, HCC), HepG2 SMMC-7721 Angiogenic factors
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