研究了茶苗南方根结线虫（Meloidogyne incognita）虫口密度的季节消长规律和在不同深度根系中的分布，以及茶圃杂草作为其保虫寄主的感染情况。感染率10～12月最高，8月最低，平均感染强度10月最高，7月最低；从上到下在第10～15cm处雌虫最多，第20～25cm侵袭期幼虫晟多；10种杂草感染该虫种。虫口密度随月积温的上升而上升，而雄性比则随月总雨量的升高而下降。

在番茄、辣椒和鸡冠花中进行的盆栽试验表明：穿剌芽孢杆菌对感染根结线虫的植物都有保护效果，大约可使虫口密度降低75%～80%；同时该菌使根结线虫致根变重的情况有所缓和，从而根冠比倒转的症状有很大改善，各组试验的解剖结果证明，穿剌芽孢杆菌的寄生都导致虫瘿、卵块、雌虫、雄虫以及各期幼虫明显减少。

The present communication reports the infection of Hirschm anniella o-ryz aewith Bacillus penetrans in Yuan Kou Village of Nan Ping city in Fujian province, which was first found in China. The infect ivityw as 5.26%. In addition, the cuticles of 40% of H. oryzae were adhered to the endospores of B. penetrans. After the infection, the pseudo-coelom of H. oryzae was filled with many spores, so that nematodes were clumsy in move-ment, body's colour was especially white, and ophagus became indistinct. When the nem a-tode bodies were cut, many spores flowed out of the wound, and the genital glands in the pseudocoelom shrivelled. The spores were (n=36) 4.1±0.1 (3.6～4.3) Lmin diameter with an in ternal, dense, spherical structure at apex which was (n=32) 2.0±0.1 (1.6～2.1) Lm in diam eter. Their morphology was similar to the spores from the females of Meloidogyne spp: This discovery indicated that B. penetrans currently appeared to have great potential for biological control of Hirschm anniella spp. of rice.

In order to make use of B. penetrans the preparation of these bacteria was prepared successfully. Each gram of the specimen contains more than 3.1×10 8 spores according to determination by bloodcount plate. The method of its production are: First, infect the larvae of root-knot nematodes with the spores of B. penetrans. Second, inoculate infectable plants with the infected larvae. Third, beat the root-systems of inoculated plants after adding some water. The flowed liquid from a bronze sieve was mixed with the clear water, filtered, precipitated, air-dried under 5～10℃, and grinded into fine powder, When these powder was added into the water which contained the larvae of root-knot nematodes, the spores of B. penetrans adhered to the cuticles of these larvae in 24h. The pot experiments of tomato, chilli and cockscomb showed that the galls, egg-sacs, females, males and all-stages of larvae produced by the plant inoculated with the larvae infected with B. penetrans are about 75%～80% less than that without these bacteria. Thus it can be seen that the spores of B. penetrans can depress the populations of root-knot nematodes obviously. Therefore, it is a promising preparation for biological control.

在厦大校园和鸿山公园检查了15株吊灯花的根结线虫受穿刺芽孢杆菌感染的情况，株感染率为100%，用漂浮法共检查根周土壤的322只幼虫，感染率为58.4%，感染强度为8.9（1～200）个孢子/幼虫；用孵化法共检查从卵块孵出的390只幼虫，感染率为42.6%，感染强度为4.8（1～100）个孢子/幼虫。虽然两种方法都有效，但前者优于后者。迄今为止，吊灯花是自然界中穿刺芽孢杆菌感染最严重的一种寄主，其大面积自然感染表明吊灯花可作为制造穿刺杆菌菌剂原料的优良树种，有希望投入应用，进行大量生产。