目的 研究探讨RhoC基因mRNA和蛋白的过量表达与肝细胞癌（HCC）的发生发展和侵袭转移的关系。方法 采用逆转录聚合酶链反应（RT2PCR）和Western印迹法，检测25例HCC及其癌旁肝组织、4例门静脉主干癌栓或肝外转移灶RhoCmRNA和蛋白的表达；同时采用PCR产物单链构象多态性（PCR2SSCP）银染法检测RhoC基因的突变情况。结果 HCC组织和癌旁肝组织中均可检测到RhoC基因mRNA和蛋白的表达， RhoCmRNA在HCC组织中的表达较癌旁肝组织高，其吸光度（A）比值分别为1.8±1.1和1.0±0.7，差异有显著性（P<0.01）；RhoC蛋白在HCC组织中的表达同样高于癌旁肝组织，其A比值分别为33992±10384和17342±9998， 差异有显著性（P<0.01）。4例门静脉主干癌栓或肝外转移灶中的RhoC基因mRNA和蛋白的表达量分别为3.3±0.5和63865±9116，较HCC肝内病灶高，差异有显著性（P<0.01）；RhoCmRNA和蛋白在分化较差、结节数较多、有静脉浸润或伴有肝外转移灶的HCC中过量表达（P<0.05）。全部HCCCR2SSCP银染法分析均未发现异常泳动条带。结论 RhoC基因的过量表达与HCC的发生发展和侵袭转移密切相关，且可能是通过过量表达而非突变发挥作用。

AIM: To investigate the role of tri-iodothyronine supplement in protecting gut barrier in septic rats. METHODS: Twenty-two rats were randomized into three groups: sham group (n=6), sepsis group (n=8), and sepsis plus tri-iodothyronine (T3) group (n=8). Septic rat model was established through cecal ligation and puncture (CLP). After 5 h, sham and sepsis groups received saline, and the remaining group received T3 intraperitoneally. Twenty-one hrs After CLP, intestinal permeability and serum free T3 and T4 were measured with fluorescence spectrophotometer and by radioimmunoassay, respectively. Intestinal ultrastructure and histologic morphology were observed under transmission electron microscopy (TEM) and light microscopy, respectively. RESULTS: After 21 h, septic symptoms and signs in sepsis plus T3 group were milder than those in sepsis group. Serum FT3 or FT4 concentration in sepsis group was lower than that in sham group (1.59