A novel plant tissue-based chemiluminescence (CL) biosensor for glycolic acid combined with flow injection analysis is proposed in this paper. The spinach tissue acts as the molecular recognition element. Glycolic acid is oxidized by oxygen under the catalysis of glycolate oxidase in the tissue column to produce hydrogen peroxide, which can react with luminol in the presence of peroxidase of spinach tissue to generate a CL signal. The CL emission intensity was linear with glycolic acid concentration in the range of 4

A simple and sensitive flow-injection chemiluminescence (CL) system for automated dissolution testing is described and evaluated for monitoring of dissolution profiles of isoniazid tablets. The undissolved suspended particles in the dissolved solution were eliminated via on-line filter. The novel CL system of KIO4-isoniazid was also investigated. The sampling frequency of the system was 120h−1. The dissolution profiles of isoniazid fast-release tablets from three sources were determined, which demonstrates the stability, great sensitivity, large dynamic measurin grange and robustness of the system.

A novel chemiluminescence (CL) flow-through sensor for hydrogen peroxide is described. It is prepared by immobilizing hemoglobin (Hb) with the sol-gel method on the flow cell, and by electrostatically immobilizing luminol on strongly basic anion-exchange resin. Hydrogen peroxide is sensed by the CL reaction with Hb sensitive membrane and luminol bleeding from the ion-exchange column with immobilized luminol by hydrolysis. The calibration graph is linear in the range of 6×10−5 to 4×10−7 mol l−1 with a detection limit of 1.3×10−7 mol l−1 (3σ). A complete analysis could be performed in 1 min with a relative standard deviation of 1.7% for 5×10−6mol l−1 H2O2 (n=9). The sensor has been applied successfully to the determination of hydrogen peroxide in rainwater.

The novel flow chemiluminescence (CL) system for determination of pipemidic acid was proposed, which was based on the sensitizing effect of pipemidic acid on the CL oxidation of sulfite by KMnO4 in acid media. Combined with the technique of ultrafiltration, the flow-injection CL system was applied to study in vitro the bovine serum album (BSA) binding of pipemidic acid. The estimated association constant (K) and the number of the binding site (n) on one molecule of BSA were 8.81

A novel plant tissue-based chemiluminescence (CL) biosensor for dopamine combined with flow injection analysis is presented in this paper. The potato roots act as molecular recognition elements. Dopamine is oxidized by oxygen under the catalysis of polyphenol oxidase in the tissue column to produce hydrogen peroxide, which can react with luminol in the presence of peroxidase of potato tissue to generate CL signal. The CL emission intensity was linear with dopamine concentration in the range of 1×10-5-1×10-7g/ml and the detection limit was 5.3×10-8g/ml (3σ) with a relative standard deviation of 1.7%. Combined with microdialysis sampling, the biosensor was applied to monitor the variation of dopamine level in the blood of rabbit after the administration of dopamine to demonstrate the favorable resolution and reliability of the system for in vivo on-line monitoring.