Usiltg PER-RFI. P and DNA sequencing，this study confimaed that Hinfl polymorphism in the ovine mitochondfiaI Colgene resldted from the T-C substitmion at the mwleollde 234 bul Ihis mutation did nol encode anolher amino acid. which was actually a synonymous mutation This single nueleotide ldymorphism can be used as gene typing marker for mitochrmdrial gerrame in the research of the internctions bctween mitochandfa and mucleus, extranuelear gene effects. and as mdecular discrimninatnig marker for embryo or individual in the research areR of gene Iransfer and animal cloning.

The 3'-terminal flanking region of porcine un-coupling protein 3 (UCP3) was cloned, the sequence data revealed 15 nucleotide substitutions among Landrace and three Chinese native pig breeds named Neijiang, Minpig and Erhualian. The continuous 9 polymorphic sites were checked bv PCR. RFLP. the results indicated that Erhualian had ex-traordinary gene frequency, presented most significant dif-ference by X2 test compared with Landrace, Largewhite, Neijiang and Minpig respectively, significant level compared with Meishan; and Meishan also had significant difierence compared with Landrace and Minpig respectively. These results can be concluded that Taihu pigs have special genetic characteristics among pig breeds.

Paternal inheritance of mitochondria DNA in sheep was discovered by examination of 152 sheep from 38 hybrid families for mtDNA D-loop polymorphisms using PCR-RFLP, amplifica-tion of repeated sequence somain, and PCR. SSCP of the D-loop 5' end region of a 253 bp frag-ment. Our findings have provided the first evidence of paternal inheritance of mtDNA in sheep and possible mechanisms of paternal inheritance were discussed.

Uncouding proteins ale, mitochondrial membrane transporters, which regulate metabolic pathways of energy balance, and ate associated with biological traits of animal body weight, resting metabolic Tares and energy conversion In this study, a region of the exons 3 and 4 ofpig UCP2 gene was cloned and analyzed, and a new single nudeotide polymorphic site was detected by PCR SSCPinfive pig breeds This newfound polymorphism Tesmts from a T to G substitution at the position of nudeotide 272, which is located in intron3.