近年来无标记型DNA传感技术的研究已成为病原基因测定和基因疾病诊断等领城新的研究热点之一。基于磁性微球分离和富集的方法，建立了一种新型的光标记化学发光检测技术，并成功地应用于特定序列DNA——端粒的检测。首先采用dT20修饰的磁性微球，与连接有dA20的捕获探针DNA杂交，然后再与端粒进行第二步杂交反服。磁性分离就涤后，利用端粒中富含的G碱基与3，4，子二二甲氧基苯乙二酸反应产生特异性化学发光，从而实现特定序列DNA——端粒的光标记检测。实验结果表明:该法具有操作简便、分析快速、灵敏度高、专属性好等特点。目标DNA浓度在5×10-9～1×10-1mol/L浓度范围内具有良好的线性关系，相关系数为0.9918。

化学发光技术在生物技术、分子生物学、药学、临床和环境等许多领域都获得了广泛的应用，有关化学发光的评论文章近年来也有许多报道。本文就化学发光技术在蛋白质和DNA分析中的较有特色的新进展（2000年以来）进行了评述，引用文献30篇。

First published as an Advance Article on the web 20th October 2003 For the first time we report on the detection of telomeres by coupling of the label-free guanine CL detection route with an efficient magnetic isolation of the hybrid.

The chemiluminescence of the luminol-H2O2 system is found for the first time to be remarkably enhanced by the Ce(IV) complexes of cyclodextrin dimers.

A novel, sensitive chemiluminescent (CL) immunoassay has been developed by taking advantage of a magnetic separation/mixing process and the amplification feature of colloidal gold label. First, the sandwich-type complex is formed in this protocol by the primary antibody immobilized on the surface of magnetic beads, the antigen in the sample, and the second antibody labeled with colloidal gold. Second, a large number of Au3+ ions from each gold particle anchored on the surface of magnetic beads are released after oxidative gold metal dissolution and then quantitatively determined by a simple and sensitive Au3+-catalyzed luminol CL reaction. Third, this protocol is evaluated for a noncompetitive immunoassay of a human immunoglobulin G, and a concentration as low as 3.1 10-12M is determined, which is competitive with colloidal gold-based anodic stripping voltammetry (ASV), colorimetric ELISA, or immunoassays based on fluorescent europium chelate labels. The high erformance of this protocol is related to the sensitive CL determination of Au3+ ion (detection limit of 210-10M), which is 25 times higher than that by ASV at a singleuse carbon-based screen-printed electrode. From the analytical chemistry point of view, this protocol will be quite promising for numerous applications in immunoassay and DNA hybridization.