内皮细胞位于血流与血管壁之间，内皮细胞调控的机械力相关反应已成为正常血管反应的一部分。切应力在调节内皮细胞功能上具有重要作用。流体切应力可以直接调节内皮细胞基因的表达，其中包括诱导内皮细胞表达IL28，而且IL-8的表达量与切应力作用时间有关。为阐明内皮细胞IL-8基因的表达除了与切应力的作用时间有关外还与切应力的强度有关，我们用不同强度的流体切应力（2.23、4.20、6.08、8.19、9.67、12.15、14.40、16、87、19.29 dyne/an2）处理培养的人脐静脉内皮细胞，然后采用定量RT-PCR 的方法检测内皮细胞IL-8基因的表达情况。结果显示：未用切应力处理的内皮细胞没有IL-8基因的表达；切应力处理内皮细胞后，低切应力（2. 23 dyne/an2）时IL-8mRNA表达量明显增加为高切应力（19.29dyne/an2）时IL-8mRNA表达量的约68（作用1h）或52倍（作用2h）。IL-8 mRNA的表达量与内皮细胞所施加的切应力强度呈反变关系；直线回归方程：1h时为y=7.57-0.11x，相关系数r=0.97；2h时为y=7.92-0.10x，相关系数r=0.96。式中：y为切应力作用下内皮细胞IL-8mRNA的表达量（拷贝数的对数值）；x为施加于内皮细胞的切应力强度（dyne/am2）。不同的切应力作用时间（1h、2h）均表现出相同的IL-8mRNA随切应力强度的变化规律。提示流体切应力诱导内皮细胞表达IL-8，不仅与切应力的作用时间有关，而且IL-8 的表达量与切应力强度有关。流体低切应力诱导内皮细胞IL-8mRNA的表达急剧增高，可能在炎症和动脉粥样硬化的发生、发展过程中具有重要作用。

采用Low Shear-30流变测定仪，改变人血红细胞压积（HCT），血浆浓度和正弦振荡剪切流频率，观察血液粘弹特性的变化。建立了经验方程，以表示HCT与粘性分量（η'），弹性分量（η"）的关系。

We investigated neutrophil activation, specifically F-actin content and distribution, in situations mimicking the in vivo environment using steady and oscillatory shear. Under low steady shear (<150 s−1) F-actin levels were decreased for both treated (n-formyl-L-methioryl-L-leucyl-L-phenylalanine (fMLP)) and untreated neutrophils. The F-actin content increased with a change to higher steady shear levels. Neutrophils show the same behavior of decreased F-actin content for oscillatory shear (26.7 s−1) as they did for steady shear. In both situations, the low shear levels caused a decrease in F-actin content. However, as the magnitude of the shear rate increased, cells showed a reversal to increasing F-actin content. Shear caused a decrease in F-actin in the cell cortex for both control and fMLP treated cells. Ctyochalasin B (CB), a common F-actin assembly blocker, significantly decreased F-actin content. The results indicate that neutrophils regulate their actin network based on the level and type of shear stress they encounter in the bloodstream.

The thixotroplc parameters of whole blood from 314 healthy subjects (154 women, 160 men) were measured with our modified method by Low shear 30 Rheometer and calculated according Huang's equation. This communication offered the reference range of thixotropic parameters from man and woman group. The results demonstrated that no significant differences existed in the plasma viscosity and fibrinogen between man and woman group. Man group had statistical-ly higher values in HCT, yield stress (to), Newtonlan contribution of viscosity (μ), non-Newtonian contribution of viscosity (ηs-μ), apparent viscosity at 2.37 sec-1 (ηs), the equi-librium value of the structual parameter (A) and apparent kinetic rate constant of rouleaux breakdown (ARC) than those in woman group. The man and woman groups could be sepa-rately divided into five subgroups in terms of age. It was found that the levels of fibrinogen and plasma viscosity had a tendency of increasing with aging. In the old subgroup (>60 years) of men and women HCT, to, ηs, μ, (ηs-μ) and A had significant lower values than those in young and middle-age subgroups. However, It was Very interested that there were differences of ARC versus age between man group and woman group, i.e. ARC in the man subgroup III, IV had lower and the woman subgroup I, III, IV had higher values than their respective older subgroup did.

探讨四环素2雌酚酮（XW630）是否对实验性骨折愈合具有促进作用。在120只新西兰大白兔实验性桡骨骨折愈合过程中给予XW630、雌酚酮（E2）、四环素（T）和生理盐水（对照组C）治疗；采用肌肉注射与伤口局部两种给药方式。测定了骨折术后30天、60天和90天愈合骨的弯曲和压缩强度，以及骨折局部骨痂中胶原和钙的含量。结果显示：在各不同时段，XW630肌注组兔桡骨的抗弯能力与抗压能力明显高于其它各组。在骨折后30天，其弯曲破坏极限的增加最为明显。分别比C组、E2组和T组高出80%、76%和69%。而在骨折后90天，XW630肌注组的桡骨弯曲破坏极限已达正常的80%，分别比C组、E2和T组高出30%、34% 和1915%。骨痂胶原含量和骨钙含量的变化与骨抗弯曲能力的变化一致。说明XW 630肌肉注射具有明显的促进骨折愈合的作用。采用局部给药方式其各组间无明显差异。在同一时段相同药物组中，肌肉注射给药方式对骨折愈合的作用明显优于局部给药方式。